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- MakaleAçık Erişim2012Scopus SJRQ2Chemotherapeutic Agent-induced Nephrotoxicity in Rabbits: Protective Role of Grape Seed Extract
Benzer, Fulya; Kandemir, Fatih Mehmet; Ceribasi, Songul; Ozkaraca, Mustafa; Yildirim, Nuran Cikcikoglu; Ozan, Sema Temizer
Cisplatin (CP) is one of the most effective cancer chemotherapeutic agent used against various solid tumors. This study was designed to investigate the antioxidant effects of Grape Seed Extract (GSE) on CP induced oxidative damage in rabbit kidney. The material of study was consist of 18 male New Zealand rabbit. Animals were divided into 3 groups. The first group was administered a single intraperitoneal (i.p.) close of 0.9% saline. The second group of rabbits was treated with CP (a single i.p. dose of 5 mg kg(-1) body weight); The third group of rabbits was treated with GSE by gavage at the dose of 250 mg kg(-1) body weight for 6 consecutive days before and 6 consecutive days after a single i.p. CP injection. As a result of CP administration, kidney function tests; urea, BUN (Blood Urea Nitrogen) and creatinine levels were increased (p<0.001). It was observed that CP increase malondialdehide (MDA) levels, the most important indicator of oxidative damage and decreased the activity of catalasc (CAT). Administration of CP did not caused statically a change in glutathione (GSH) levels and glutathione peroxidase (GSH-P-x) activity (p>0.05). GSE treatment has high levels of MDA to compared to normal levels (p<0.001) but not an increase in CAT (p<0.05). In CP treatment group, severe acute tubular necrosis was seen. It was determined that in CP+GSE group mild degenerative changes were received instead of acute tubular necrosis. GSE has been found to be partially protective effect against CP induced biochemical and histopathological changes in rabbit kidney.
AtıfWoS7OpenAlex7
İncele - MakaleYalnız künye2011Scopus SJRQ3Effect of Pomegranate Seed Extract on Free Radical Damage and Antioxidant Activity Under Cisplatin-Induced Oxidative Stress Conditions in Rabbit Testes
Benzer, F.; Kandemir, F. M.; Yildirim, N. C.; Ozan, S. T.
The aim of this study is to evaluate the protective effect of pomegranate seed extract on cisplatin-induced oxidative stress in rabbits testes. Rabbits were divided into three groups; (i) being control group, (ii) cisplatin group and (iii) cisplatin + pomegranate seed extract group. The level of malondialdehyde and glutathione, the activity of catalase, glutathione peroxidase, were measured in testes tissue of rabbits. Malondialdehyde levels were increased depending on cisplatin. In the case of cisplatin + pomegranate seed extract, malondialdehyde levels were found to be lower than cisplatin group (p < 0.05). In cisplatin group, catalase, glutathione peroxidase activity and glutathione levels were decreased when compared to control but in cisplatin + pomegranate seed extract group, catalase, glutathione peroxidase activity and glutathione levels were found higher than cisplatin group (p < 0.05). The pomegranate seed extract supplementation may play a protective role or decrease the side effects of cisplatin-induced testes toxicity from cancer medicines.
AtıfWoS5İncele - MakaleYalnız künye2011Scopus SJRQ2Compensatory effects of curcumin on cisplatin-induced toxicity in rabbit testis
Kandemir, F. M.; Benzer, F.; Yildirim, N. C.; Ozdemir, N.
Curcumin, a widely used spice and colouring agent in food, has been shown to possess potent antioxidant, antitumor promoting and anti-inflammatory properties in vitro and in vivo. The present study was designed to investigate the protective effects of curcumin on changes in the levels of lipid peroxidation and endogenous antioxidants induced by cisplatin (cis-diamminedichloroplatinum II, CDDP) in the testiclular tissue of rabbits. 18 healthy male New Zealand white rabbits were equally divided into three groups of six rabbits each, control, cisplatin, and cisplatin+curcumin. The degree of protection produced by cisplatin was evaluated by determining the level of malondialdehyde (MDA) and glutathione (GSH), the activity of catalase (CAT), glutathione peroxidase (GSH-Px), were estimated from testes homogenates. MDA levels were increased with cisplatin compared to control but in cisplatin+curcumin group, MDA levels were found to be lower than cisplatin group (p<0.05). The activity of CAT and GSH-P-X was decreased in cisplatin and cisplatin+curcumin groups compared to control (p<0.05). In the case of cisplatin+curcumin CAT and GSH-Px activity were increased compared to cisplatin group (p<0.05). GSH levels were decreased with cisplatin but administration cisplatin+curcumin increased the levels of GSH comared to cisplatin group (p<0.05). In the present study, co-administration of curcumin with cisplatin prevented the damage to testes induced by this drug and may be considered as a potentially useful candidate in the combination chemotherapy with cisplatin.
AtıfWoS22İncele - MakaleYalnız künye2011Scopus SJRQ2BENEFICIAL EFFECTS OF GRAPE SEED EXTRACT AGAINST CISPLATIN-INDUCED TESTICULER DAMAGE IN RABBITS
Yildirim, Nuran Cikcikoglu; Kandemir, Fatih Mehmet; Benzer, Fulya
Biological compounds with antioxidant properties may contribute to the protection of cells and tissues against deleterious effects of reactive oxygen species (ROS) and other free radicals induced by cisplatin. This study was performed to investigate the possible protective role of antioxidant treatment with grape seed extract (GSE) on cisplatin-induced testes oxidant injury using biochemical approaches. The degree of protection produced by GSE was evaluated by determining the level of malondialdehyde (MDA) and glutathione (GSH), the activity of catalase (CAT), glutathione peroxidase (GSH-Px), were estimated from testes of rabbits. Male New Zealand white rabbits were divided into 3 groups (n=6): 1-Control group(1 ml saline. i.p) 2-Cisplatin group (a single dose of cisplatin (5 mg/kg, i.p) 3-A single dose of cisplatin (5 mg/kg, i.p) + GSE (250 mg/kg/day, i.p) for 6 days. MDA level were significantly higher when compared to Control (p < 0.05). In Cisplatin+grapes groups MDA levels were found significantly lower than cisplatin group (p < 0.05). GSH levels were decreased with cisplatin compared to control but in case of cisplatin+ GSE it increased (p < 0.05). In treated rabbits, the activity of CAT and GSH-Px was decreased in cisplatin and cisplatin+ GSE groups compared to control (p < 0.001). These results indicate that the antioxidant GSE might have a protective effect against cisplatin-induced testiculer damage and oxidative stress in rabbit.
AtıfWoS12İncele