Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Gunay, C.(2)
- Han, M. C.(2)
- Kandemir, F. M.(2)
- Ozkaraca, M.(2)
- Sagliyan, A.(2)
- Aslan korkmaz, Şengül(1)
- Benzer, E.(1)
- Benzer, F.(1)
- Doğan, Üzeyir(1)
- Gallo, Monica(1)
- Gašić, Uroš(1)
- Güçlü, Ebru(1)
- Ivanov, Marija(1)
- Jugreet, Bibi Sharmeen(1)
- Korkmaz, Nesrin(1)
- Mahomoodally, Mohamad Fawzi(1)
- Montesano, Domenico(1)
- Paksoy, Mehmet Yavuz(1)
- Stojković, Dejan(1)
- Yaman, Cennet(1)
- Zengin, Gokhan(1)
- İmamoğlu, Rızvan(1)
Yayınlar
- MakaleYalnız künye2026Scopus SJRQ2Comparative phytochemical characterization and bioactivity evaluation of hemp (Cannabis sativa L.) root extracts using green extraction technologies
Korkmaz, Nesrin; Yaman, Cennet; Güçlü, Ebru; Doğan, Üzeyir; İmamoğlu, Rızvan; Aslan korkmaz, Şengül
This study investigates the largely untapped therapeutic potential of industrial hemp (Cannabis sativa L.) roots, which are usually discarded as agricultural byproducts. The phytochemical composition and biological activities of extracts obtained using Soxhlet, ultrasound-assisted extraction (UAE), and supercritical CO₂ (SC-CO₂) techniques were comparatively evaluated. Compounds were characterized using FTIR, GC–MS, LC–MS, and ICP-MS. SC-CO₂ extraction yielded the most chemically diverse profile, particularly enriched in fatty acids and triterpenoids. In vitro assays demonstrated that SC-CO₂ and Soxhlet extracts significantly promoted fibroblast migration and reduced inflammatory responses compared to UAE extracts. Gene and protein expression analyses further supported these bioactivities. In conclusion, this study highlights the phytopharmaceutical potential of hemp root extracts obtained using alternative extraction techniques, including supercritical CO₂ extraction. While SC-CO₂ extraction enabled the recovery of a chemically diverse extract, the present study focuses on comparative phytochemical and bioactivity outcomes rather than a quantitative assessment of environmental sustainability. The findings support further investigation of hemp roots, currently treated as an underutilized biomass, for potential applications in natural product research.
AtıfOpenAlex1
İncele - MakaleAçık Erişim2021Scopus SJRQ1Comprehensive biological and chemical evaluation of two seseli species (S. gummiferum and s. transcaucasicum)
Zengin, Gokhan; Stojković, Dejan; Mahomoodally, Mohamad Fawzi; Jugreet, Bibi Sharmeen; Paksoy, Mehmet Yavuz; Ivanov, Marija; Gašić, Uroš; Gallo, Monica; Montesano, Domenico
Seseli L. is one of the largest genera of the Apiaceae family widely known for their traditional uses as herbal remedies. In the present study, the methanolic and water extracts of two Seseli species, S. gummiferum and S. transcaucasicum were evaluated for their bioactive contents and biological activities. The total phenolic and flavonoid contents in the extracts ranged from 19.09 to 24.33 mg GAE/g and from 0.45 to10.09 mg RE/g, respectively. Moreover, while narcissin was detected as the most abundant component in the methanolic extract of S. transcaucasicum (261.40 µg/g), chlorogenic acid was identified as the major component in all the other extracts, although a high amount was also present in the methanolic S. transcaucasicum extract (107.48–243.12 µg/g). The total antioxidant capacity was also determined by the phosphomolybdenum assay (0.66–1.18 mM TE/g). Other antioxidant assays such as the radical scavenging assays (DPPH: 5.51–11.45 mg TE/g; ABTS: 43.46–51.91 mg TE/g), reducing assays (CUPRAC: 41.67–53.20 mg TE/g; FRAP: 31.26–34.14 mg TE/g), as well as the metal chelating activity assay (14.38–38.57 mg EDTAE/g) were conducted. All the extracts showed inhibitory potential against the enzyme’s amylase (0.12–0.78 mM ACAE/g), acetyl-and butyryl-cholinesterase (0.15–9.71 mg GALAE/g), while only the methanolic extracts acted as inhibitors of tyrosinase (107.15 and 109.37 mg KAE/g) and only the water extract of S. gummiferum displayed anti-glucosidase activity (0.13 mM ACAE/g). Interestingly, the methanolic extracts of both Seseli species showed lower cytotoxicity towards HaCaT cells (IC50: >500 µg/mL), compared to the water extracts (IC50: 267.8 and 321.41 µg/mL). Besides, only the methanolic extracts showed a slight wound healing effect (28.21 and 31.23%). All extracts showed antibacterial action against Staphylococcus lugdunensis (minimum inhibitory and bactericidal concentrations: 0.025–2 mg/mL). S. gummiferum methanolic extract, which exhibited the highest antibacterial potency was found to inhibit adhesion and invasion of S. lugdunensis to HaCaT cells as well. Taken together, this study demonstrated the two Seseli species to harbour interesting bioactive components, in particular polyphenolics and to exhibit several biological properties that could be further investigated for their potential exploitation as healing agents as supported by various traditional medicinal uses.
AtıfScopus16OpenAlex15
İncele - MakaleYalnız künye2013Scopus SJRQ3Effects of oral administrations of pomegranate seed extract on surgical wound healing in rabbits
Kandemir, F. M.; Sagliyan, A.; Ozkaraca, M.; Gunay, C.; Han, M. C.; Benzer, F.
This study examined the effects of orally given pomegranate seed extract (PSE) on healing of surgically induced full thickness skin wounds in terms of clinical, histopathological and biochemical aspects. A total of 48 healthy, adult male New Zealand rabbits were randomly divided in 2 equal experimental (PSE group) and control groups (C): one week prior the surgery, 100 mg/kg per day via gastric catheter pomegranate seed extract was orally given for 20 days to the assay animals. After one week, all rabitts were anaesthetized by intramuscular injection of xylazine HCl (5 mg/kg) and ketamine HCI (50 mg/kg) and after shaving and disinfection, a circular full thickness skin defect of 2.5 cm diameter was incised on the median line of the back area. Macroscopic and histopathological aspects as well as the tissue oxidant/antioxidant balance were determined on 6 rabbits from each group slaughtered at the 5th, 10th, 15th and 20th days post-surgery.
AtıfWoS9İncele - MakaleYalnız künye2012Scopus SJRQ3Beneficial effects of oral administrations of grape seed extract on healing of surgically induced skin wounds in rabbits
Sagliyan, A.; Benzer, E.; Kandemir, F. M.; Gunay, C.; Han, M. C.; Ozkaraca, M.
This study examined the effects of orally given grape seed extract (GSE) on healing of surgically induced full thickness skin wounds in terms of clinical, histopathological and biochemical aspects. A total of 48 healthy. adult male New Zealand rabbits were randomly divided in 2 equal experimental and control groups: one week prior the surgery, 260 mg/kg grape seed extract was orally given for 20 days to the assay animals. A circular full thickness skin wound (2.5 cm in diameter) was made in the back midline of all rabbits under anaesthesia then, macroscopic and histopathological aspects as well as the tissue oxidant/antioxidant balance were determined on 6 rabbits from each group slaughtered at the 5th, 10th, 15th and 20th days post-surgery. In treated rabbits, it was clinically observed that the wound contraction was significantly amplified and the histological processes of re-epithelisation, granulation tissue development, collagen accumulation and angiogenesis were markedly hastened whereas the inflammatory reaction and the ulcer disappeared more rapidly than in the not-treated controls. Furthermore, the maximal skin MDA formation observed on the 10th day was significantly depressed and the antioxidant catalase (CAT) and glutathione peroxidase (GSH-Px) activities remained significantly elevated until the 15th day compared to the controls. These results demonstrate the beneficial effects of treatment with GSE on skin wound healing in rabbits.
AtıfWoS4İncele