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- MakaleYalnız künye2020Scopus SJRQ4Metal bioaccumulation in selected tissues of barb (Barbus sp.) and common carp (Cyprinus carpio, Linnaeus 1758) from the Keban Dam Lake, Turkey
Danabas, Durali; Kutluyer, Filiz; Ural, Mesut; Kocabaş, Mehmet
The aquatic life has been negatively influenced by harmful effects of environmental toxic elements, including heavy metals. The elevated concentrations of metals may be harmful for aquatic animals and human health. Herein, the present study deals with assessment of the bioavailability of metals [cadmium (Cd), chrome (Cr), copper (Cu), zinc (Zn), iron (Fe), lead (Pb), manganese (Mn), mercury (Hg), nickel (Ni), and selenium (Se)] in organs such as muscle, gills, liver, kidney, and gonad of the commercially consumed Barbus sp. and Cyprinus carpio (Linnaeus, 1758) collected from Keban Dam Lake, eastern Turkey. The fish were captured from four different stations of Keban Dam Lake. Our results indicated that Fe and Zn concentrations were the highest in all tissues of two fish species while Cd concentrations were the lowest levels. In addition, detected metals accumulated in the muscle of common carp at higher levels compared to Barbus sp. The results from the present study also demonstrated significant variations and considerable differences in the concentrations of all 10 elements between the four stations and different fish tissues. Overall, the concentrations of detected metals in the muscle were many folds higher than the permissible level prescribed by some national and international legislation, which raises health risk concerns.
AtıfScopus11WoS9OpenAlex14
İncele - MakaleAçık Erişim2019Scopus SJRQ2Improvement of sperm motility of Oncorhynchus mykiss and Salvelinus fontinalis by L-tryptophan
Kocabaş, Mehmet; Kutluyer, Filiz; Ertekin, Özlem; Aksu, Önder; Başçınar, Nadir
Experiments were designed to test the influence of L-tryptophan supplementation of the extender on the sperm motility parameters and bacterial flora of brook trout (Salvelinus fontinalis) and rainbow trout (Oncorhynchus mykiss). The extender containing 0.3 M glucose in 10% Dimethyl sulfoxide (DMSO) was supplemented with 0, 0.5, 1, 2, and 4 mM L-tryptophan. Sperm samples stored for up to 12 days at 4°C for brook trout were motile while motility was not observed after six days of storage for rainbow trout. Diluted sperm samples were spread-plated onto Plate Count Agar (PCA) (Total Bacteria Count), Rose Bengal Agar (RBC) (Yeast-Mold Count), Violet Red Bile Dextrose (VRBD) (Enterobacteriaceae count) and Mannitol Salt Agar (MSA) (Micrococcus/Staphylococcus count) and counts were performed in duplicate and sampling occurred on day 0, 2, 4, 6, and 12 of cold storage. L-tryptophan supplementation of the extender yielded a positive effect, significantly prolonging sperm motility in S. fontinalis and O. mykiss compared to the control group (p < 0.05). The ideal results were obtained above a concentration of 0.5 mM for both species. Total bacteria count in fresh sperm (undiluted samples) was not detectable and not detected in sperm samples treated with different L-tryptophan concentrations. Yeast-mold, Enterobacteriaceae and Micrococcus/Staphylococcus were not detected from fresh and treated sperm brook trout and rainbow trout. On the basis of the results, L-tryptophan-based extender is useful for maintaining sperm quality in short-term storage. L-tryptophan had a negative effect on the bacterial flora. The results of the current study encourages further studies related to long-term storage and reproduction management. Abbreviations: DMSO: dimethyl sulfoxide; PCA: plate count agar; RBC: rose Bengal agar; VRBD: violet red bile dextrose; MSA: mannitol salt agar; SCA: sperm class analyzer; CFU: colony-forming unit.
AtıfScopus13WoS14OpenAlex13
İncele - MakaleAçık Erişim2019Scopus SJRQ3Effect of the organophosphate insecticide chlorpyrifos exposure on oxidative stress and quality of Salmo coruhensis spermatozoa
Kutluyer, Filiz; Kocabaş, Mehmet; Erişir, Mine; Benzer, Fulya
The use of pesticides has been increasing along with increasing farming activities and has caused deleterious environmental impacts. Non-target organisms in particular, including fish, are affected by pesticides. In this work, the impacts of Chlorpyrifos (CPF) on sperm oxidative stress markers and sperm motility were investigated in vitro. CPF concentrations were 0 μg/L (control), 5 μg/L, 10 μg/L and 15 μg/L. Lipid peroxidation [malondialdehyde (MDA)], nonenzymatic antioxidants [glutathione (GSH)] and enzymatic [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)] activities in sperm cells were examined for the determination of oxidative stress status. Our findings showed that motility and survival of sperm cells significantly decreased with exposure to Chlorpyrifos. Biochemical assays revealed that CAT activity and levels of MDA and, GSH increased in spermatozoa based on CPF concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to CPF exposure. It can be deduced that CPF has the potential to disrupt sperm quality and to cause to oxidative stress in sperm cells of S. coruhensis.
AtıfScopus15WoS14OpenAlex13İncele - MakaleYalnız künye2018Scopus SJRQ2Chemoprotective effects of curcumin on doxorubicin-induced nephrotoxicity in wistar rats: by modulating inflammatory cytokines, apoptosis, oxidative stress and oxidative DNA damage
Benzer, Fulya; Kandemir, Fatih Mehmet; Kucukler, Sefa; Comaklı, Selim; Caglayan, Cuneyt
Doxorubicin (DXR) is one of the most important chemotherapeutic agent. However, nephrotoxicity reduces its clinical utility in humans. The aim of the study was to investigate protective effects of curcumin (CMN) against DXR-induced nephrotoxicity. Rats were subjected to oral treatment of CMN (100 and 200 mg/kg body weight) for 7 days. Nephrotoxicity was induced by single intra peritoneal injection of DXR (40 mg/kg body weight) on the fifth day and then the experiment was terminated on the eighth day. Nephroprotective effects of CMN were associated with decrease in serum toxicity markers and increase in antioxidant enzyme activities. CMN was able to reduced the levels of inflammatory markers such as TNF-α, NF-κB, IL-1β, iNOS and COX-2 in the rats. It also reduced the expressions of apoptotic marker including caspase-3, and oxidative DNA damage marker including 8-OHdG. Collectively, these findings indicated that CMN protect against DXR-induced nephrotoxicity.
AtıfScopus116WoS111OpenAlex122İncele - DergiSystems Biology in Reproductive Medicine
- DergiArchives of Physiology and Biochemistry