Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Aksakal, B.(5)
- Aslan, N.(4)
- Aksakal, Bunyamin(2)
- Aksakal, Bünyamin(2)
- Demirel, M.(2)
- Demirel, Mehtap(2)
- Sezek, S.(2)
- Yilmazer, Yasemin(2)
- Aksoy, M.E.(1)
- Arslan, M.E.(1)
- Aslan, Naim(1)
- Azerigyik, Faustus Akankperiwen(1)
- Camas, Anil Sazak(1)
- Camas, Mustafa(1)
- Cetin, Fadime(1)
- Cihangir, S.(1)
- Cihangir, Salih(1)
- Deng, Hai(1)
- Dofuor, Aboagye Kwarteng(1)
- Fidan, F.(1)
- Isın, Ege(1)
- Jaspars, Marcel(1)
- Karadogan, S.A.(1)
- Kaya, Ali İhsan(1)
- Kwain, Samuel(1)
- Kyeremeh, Kwaku(1)
- Mensah, Thomas(1)
- Say, Yakup(1)
- Sinirlioglu, Zeynep A.(1)
- Tetevi, Gilbert Mawuli(1)
- Yilmazer, Y.(1)
Yayınlar
- MakaleAçık Erişim2024Scopus SJRQ2The influence of cold rolling and hydroxyapatite coating on the mechanostructure, corrosion resistance, cell viability, and antibacterial activity of ZnCu biodegradable implants
Aksakal, B.; Karadogan, S.A.; Aslan, N.; Fidan, F.; Yilmazer, Y.; Sezek, S.
Zinc (Zn) and its alloys exhibit great potential for utilization in biodegradable bone implants. Zn-1Cu biodegradable alloy was produced and were cold rolled at two different deformation rates of 47 and 61%. The samples have been bioceramic coated with the sol–gel method and microstructure-mechanical property changes, corrosion behavior and biocompatibility of the samples were investigated. They were characterized by Optical, SEM, XRD and wettability analysis. The rolled samples showed a significant increase in hardness when compared to the non-rolled samples. The unrolled and 47% rolled samples showed better corrosion resistance compared to 61% rolled samples. Antibacterial effects of the base and sol–gel-coated groups showed higher cell viability ratios than the uncoated groups. Cell viability increased significantly in 47% of the rolled samples after 24, 48, and 72 h, however decreased by up to 70% in 61% of the rolled samples. Graphical abstract: (Figure presented.).
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İncele - MakaleAçık Erişim2024Scopus SJRQ1Influence of Plastic Deformation and Hydroxyapatite Coating on Structure, Mechanical, Corrosion, Antibacterial and Cell Viability Properties of Zinc Based Biodegradable Alloys
Aksakal, B.; Isın, Ege; Aslan, N.; Cihangir, S.; Sezek, S.; Yilmazer, Yasemin
Zinc (Zn)-based biodegradable alloys have been at the forefront of absorbable biomaterial research in recent years due to their high biocompatibility and corrosion rates. The arc melting process was used to produce the Zn–1Cu–1Ag biodegradable alloy. The influence of different plastic deformation rates on the microstructure of the material was examined after the cold rolling at deformation rates of 47% and 61%. The undeformed and deformed alloys have been hydroxyapatite-coated using the electrophoretic deposition process to improve its surface, corrosion, and bioactivity properties. Optical, XRD, SEM, and EDS examinations were used to analyze the samples’ uncoated, coated, and rolled-unrolled forms. The nucleation of the (Ag, Cu)Zn4 secondary phase was formed during the rolling process. Hardness and compression tests were used to determine the mechanical properties of cast and rolled alloys, and in vitro corrosion tests were carried out in simulated body fluid. Antimicrobial and cell viability tests are executed to demonstrate the biocompatibility of the deformed and HA-coated Zn–1Cu–1Ag alloy. The mechanical properties were improved after the rolling process, with the highest results found in 47% of the rolled samples exhibiting a compressive strength of 412.65 ± 0.5 MPa and 61% of the rolled samples exhibiting a hardness value of 88.1 ± 0.5 HV. The samples that were rolled (61%) and coated with hydroxyapatite (HA) exhibited the highest level of corrosion resistance. The antimicrobial tests revealed that the rolled and HA coated Zn1Cu1Ag groups exhibited greater inhibition rates (47 and 61%) compared to the other groups when tested against E. coli. The HA-coated groups exhibited good cell viability ratios, with the maximum viability seen in the rolled and HA-coated group at 47%.
AtıfScopus4WoS5OpenAlex4İncele - MakaleAçık Erişim2023Scopus SJRQ2Fabrication of hydroxyapatite-based nano-gold and nano-silver-doped bioceramic bone grafts: Enhanced mechanostructure, cell viability, and nuclear abnormality properties
Demirel, M.; Aslan, N.; Aksakal, B.; Arslan, M.E.
In this study, nano-gold (nAu) and nano-silver (nAg) were doped at the molar ratios of Molar5–Molar30 to the Hydroxyapatite (HAp)-based bioceramic bone graft synthesized by the sol–gel method. The effects of nAu and nAg on structural, mechanical, cell viability, and nuclear abnormality of the synthesized bioceramic grafts were evaluated. The chemical and morphological properties of the bone grafts after production were examined through XRD and SEM–EDX analyses and mechanical tests. To determine the biocompatibility of the bone grafts, cell viability tests were performed using human fibroblast cells. In the cytotoxicity analyses, only HAp and HAp-nAu5 grafts did not show toxicological properties at any concentration, while HAp-nAg5 among the nAg-containing grafts gave the best results at the 200–100 μg/mL concentrations and showed significant cytotoxicity in human fibroblast cells. The other nAu-containing grafts showed toxicological properties in the concentration range of 200–50 μg/mL and nAg-containing grafts in the concentration range of 200–100 μg/mL against the negative control. The micronucleus (MN) analyses showed that the lowest total MN and L (lobbed) amounts, while the lowest total N (notched) amount, was obtained from the only HAp graft. It was found that the nAg-doped bone grafts gave higher total MN, L, and N amounts compared to the nAu-doped bone grafts. Furthermore, while the mean nuclear abnormality (NA) values of all grafts gave close results, the highest values were again obtained from the nAg-doped bone grafts.
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İncele - MakaleAçık Erişim2023Scopus SJRQ2ZrN and ta-C coatings on titanium for biomedical applications: Improved adhesion, corrosion, antibacterial activity, and cytotoxicity properties
Aslan, Naim; Aksakal, Bünyamin; Cihangir, Salih; Cetin, Fadime; Yilmazer, Yasemin
Commercially pure titanium (CP-Ti) is extensively used in biomedical applications. However, numerous qualities, including mechanical strength, corrosion resistance, and wear resistance, must be established for long-term performance as an implant material. This study investigates the enhancement of surface properties of CP-Ti through physical vapor deposition (PVD) with ZrN and ta-C (tetrahedral amorphous carbon). Among the coatings, ZrN revealed best overall results, such as adhesion, corrosion, microhardness, and wettability, excluding cell viability when compared to ta-C. E. coli and S. aureus were employed for antibacterial activity and MTS cell viability experiments. Compared to α-titanium, hardness tests revealed 91% increase in ZrN coating, and %22 increase in ta-C coating. Super-hydrophobicity and excellent antibacterial activity were found for ZrN coatings in comparison to ta-C. These findings emphasize the potential of ZrN and ta-C coatings to improve the surface properties of titanium, encouraging it to be used in biomedical devices. Graphical abstract: [Figure not available: see fulltext.].
AtıfScopus16WoS16OpenAlex19İncele - MakaleYalnız künye2022Scopus SJRQ3Boron-incorporated biocomposite coatings on 316L and NiTi alloys: Enhanced structural, antibacterial activity, and cell viability performances
Aslan, N.; Aksakal, B.; Aksoy, M.E.
Boron doped (5 %, 10%, and 15 wt.%) Hydroxyapatite (B-HA) biocomposites were syntesized and coated on 316L SS and NiTi (Ni-45Ti) metallic substrates by using the electrophoretic deposition process (EPD). The morphological and structural characterization of the coatings was executed using scanning electron microscopy (SEM) and X-ray diffraction devices (XRD). Antibacterial tests were conducted using Escherichia coli (E. coli, JM103) and Staphylococcus aureus (S. aureus, ATCC29293) microorganisms. The mitochondrial activity assay (MTT)-[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] was used to examine cell viability and cytotoxicity in Saos-2 osteoblast cells. HA and boron peaks, as well as B-TCP and metallic components, were detected in XRD examinations. Porous morphologies were generated on the surface with boron doped B-HA coatings, as revealed by SEM views. Antibacterial activity studies revealed that both metallic coating groups, notably with boron doping, demonstrated antibacterial activity against gram-negative E. coli and gram-positive S. aureus. The antibacterial activity of the 316L group was shown to be better than that of the NiTi group in comparisonal testing. The syntesized boron-doped biocomposite coatings did not have any detrimental effects on living cells, according to cell viability studies. The cell viability rate was found to be greater in NiTi coatings than in 316 SS coatings, and the impact was amplified by the addition of boron.
AtıfScopus6WoS4OpenAlex7İncele - MakaleYalnız künye2022Scopus SJRQ2Silver/selenium/chitosan co-substituted bioceramic coatings of Ni–Ti alloy: Antibacterial efficiency and cell viability
Say, Yakup; Aksakal, Bunyamin; Sinirlioglu, Zeynep A.
The effects of silver (Ag), selenium (Se), and chitosan (Cht) additives on the antibacterial activity and cell viability of NiTi were investigated. Three different hydroxyapatite (HA)-based bioceramic coatings to improve the antibacterial activity and cell viability of NiTi; HA, HA/Ag, and HA/Se–Cht were applied to NiTi substrates by a sol–gel method. The coated samples were characterized by scanning electron microscopy–electron-dispersive spectroscopy and X-ray diffraction and surface hardnesses were measured. The antimicrobial efficiency of the coatings and uncoated surfaces were tested against Escherichia coli-JM 103 and Staphylococcus aureus-ATCC29293. In vitro cell–material interactions using Saos-2 osteoblast cells were characterized by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mitochondrial activity test) assay for cell viability. A homogeneous, crack-free, and porous surface morphology is achieved in coatings. It has been shown that the Se–Cht additives did not have a significant effect on the surface hardness of the HA coating, but the Ag additive increased the hardness. Through in vitro antibacterial activity and cell viability tests, it was shown that Ag additive to bioceramic coatings significantly increased (p <.05) antibacterial properties but caused a decrease in cell viability. However, although Se–Cht additives did not have a significant effect on antibacterial properties (p <.05), it was observed that they increased cell viability.
AtıfScopus8WoS8OpenAlex2İncele - MakaleAçık Erişim2019Scopus SJRQ4Digyaindoleacid A: 2-(1-(4-hydroxyphenyl)-3-oxobut-1-en-2-yloxy)-3-(1H-indol-3-yl)propanoic acid, a novel indole alkaloid
Kwain, Samuel; Tetevi, Gilbert Mawuli; Mensah, Thomas; Camas, Anil Sazak; Camas, Mustafa; Dofuor, Aboagye Kwarteng; Azerigyik, Faustus Akankperiwen; Deng, Hai; Jaspars, Marcel; Kyeremeh, Kwaku
Digyaindoleacid A (1) is one of the novel alkaloids produced by the Ghanaian Paenibacillus sp. DE2SH (GenBank Accession Number: MH091697) isolated from the mangrove rhizosphere soils of the Pterocarpus santalinoides tree growing in the wetlands of the Digya National Park, Brong Ahafo Region, Ghana. This compound was isolated on HPLC at tR ≈ 60 min and its structure determined by MS, 1D, and 2D-NMR data. When tested against Trypanosoma brucei subsp. brucei strain 927/4 GUTat10.1, 1 produced a half-maximal inhibitory concentration (IC50) 5.21 µM compared to the standard diminazene aceturate (IC50 = 1.86 µM). In the presence of normal mouse macrophages RAW 264.7, 1 displayed a higher selectivity towards T. brucei subsp. brucei (selectivity indices (SI) = 30.2) with low toxicity. This result is interesting given that the drug diminazene aceturate is considerably toxic and 1 is a natural product isolate. The structure of 1 incorporates the backbone of the amino acid tryptophan which is crucial in the metabolism of Trypanosoma brucei subsp. brucei strain 927/4 GUTat10.1. It is possible that 1, could interfere with the normal uptake and metabolism of tryptophan in the parasite. However, 1 (IC50 = 135.41 µM) produced weak antileishmanial activity when tested against Leishmania donovani (Laveran and Mesnil) Ross (D10).
AtıfScopus4WoS3OpenAlex4İncele - MakaleYalnız künye2017Scopus SJRQ3Synthesis and characterization of Bioglass-based bone grafts with Gelatine substitution for biomedical applications
Aksakal, B.; Demirel, M.
Background: Syntesizing alternative bone graft materials are important in biomedical applications. Their morphology, mechanical properties and cell viability plays an important role in tissue engineering. OBJECTIVE: Bioglass (B) based bone grafts with Gelatin (G) substitution were syntesized via the sol-gel method and were compared with various Gelatin and Bioglass concentrations (wt%). METHODS: Syntesized bone grafts were characterized by Scanning Electron Microscopy (SEM) and X-ray diffraction (XRD) to show the structural and morphological changes of the fabricated B-based bone grafts. RESULTS: It was demonstrated that the concentration of pore size increased with increasing amounts of Gelatin in wt%. The biograft-B40G20 produced the highest flexture strength and hardness. Increasing the pore size caused a decrease in hardness and flexture stress of B-based biografts. CONCLUSIONS: Cell viability tests were conducted on the fabricated biografts and it was shown that the cell viability increased in fabricated B-based bone grafts.
AtıfScopus1WoS1OpenAlex1İncele - MakaleYalnız künye2017Scopus SJRQ1The effect of Zirconia/Yttria/Silver substitutions on mechanostructure and cell viability of the synthesized bioceramic bone grafts
Aksakal, Bunyamin; Demirel, Mehtap
Hydroxyapatite (HA) based biografts were synthesized by substitution of Zirconia (Zr), Yttria (Y2O3) and Silver (Ag) using the sol-gel method. Fabricated bone grafts (H40ZrYt and H50Yt10) were compared with a control biograft. The effects of Zr and Y2O3 and Ag additions on the morphological, mechanical and cell viability of the synthesized biografts were analyzed. The biografts were characterized by X-ray Diffractometer (XRD) and Scanning Electron Microscope (SEM). All biografts were then embedded into the drilled holes (D=3.2 mm) in rabbit femurs. The rabbits were then sacrificed after twelve weeks. Three-point bending (3-PB) tests were conducted on defected and grafted regions. Flexural strength values were found to be 347, 149 and 180 MPa, for the H40ZrYt, H50Yt10 and the control/commercial graft, respectively. Cell viability results indicated that the percentage viability ratios slightly increased after the substitution of Ag into the H50Yt10 biograft at 0.1 and 0.3 µg/ml concentrations and into the H40ZrYt biograft at a 0.1 µg/ml concentration. Although the cell viability was a bit lower in the H50Yt10 graft, the H40ZrYt graft achieved better results compared to the counterpart grafts. Furthermore, the hardness of both of the novel fabricated grafts were found to be higher than the control/commercial graft, however, as the flexture strength of H40ZrYt was higher but H50Yt10 was lower than the control/commercial graft.
AtıfScopus11WoS10OpenAlex13İncele - MakaleYalnız künye2017Scopus SJRQ2The effect and characterization of newly synthesized SrBr2 reinforced bone grafts on structure and cell viability
Demirel, Mehtap; Aksakal, Bünyamin; Kaya, Ali İhsan
Abstract: In this study, a highly durable SrBr2 compound was added to Ca(NO3)2·4H2O, KOH, NaNO3, P2O5, and urea (CO(NH2)2) compounds at different ratios (wt 10–30%); biografts were synthesized in different compositions using the sol gel method They were characterized using X-ray diffraction, Fourier transform infrared spectrometer, scanning electron microscope, and mechanical tests e.g. hardness and compression. Structural, mechanical properties and cell viabilities of the synthesized biografts were comparatively examined. Fourier transform infrared spectrometer and X-ray diffraction analysis results showed that, including SrBr2, hydroxyapatite had formed in the biografts. Also, hardness and compression tests revealed that compressive stress and hardness values of the grafts increased as the amount of SrBr2 increased. The cell viability ratios also enhanced with the amount of SrBr2 increased in the synthesized biografts. Graphical Abstract: [InlineMediaObject not available: see fulltext.]
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