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- MakaleAçık Erişim2025Scopus SJRQ4Effects of chlorpyrifos on the freshwater mussel Unio elongatulus eucirrus (Bourguignat, 1860), with a focus on neurotoxicity and oxidative stress responses; [Efectos del clorpirifos en el mejillón de agua dulce Unio elongatulus eucirrus (Bourguignat, 1860), con especial atención a la neurotoxicidad y las respuestas al estrés oxidativo]
Zorlar, Faruk; Şeker, Engin; Pala, Ayşegül
This study set out to investigate the effects of the organophosphate insecticide chlorpyrifos (CPF) on certain biochemical parameters of the freshwater mussel Unio elongatulus eucirrus. Mussels were exposed to varying concentrations of CPF (10, 20, and 40 μg·L-1) for duration of 96 hours. Samples were collected from the mussels at the 24th and 96th hours of the experiment. The results indicated that exposure to CPF concentrations led to a decline in the activities of acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities and, accompanied by an increase in malondialdehyde (MDA), and decreased reduced glutathione (GSH) levels in mussels. In conclusion, the present study demonstrates that CPF caused AChE inhibition, formation of oxidative stress, and negative effects on certain antioxidant parameters in the freshwater mussel U. elongatulus eucirrus.
- MakaleAçık Erişim2022Scopus SJRQ2Effect of chlorpyrifos on some biochemical changes in Cyprinus carpio: the protective effect of ellagic acid
Mişe Yonar, Serpil; Yonar, Muhammet Enis; Ural, Mevlüt Şener; Pala, Ayşegül
In this study, ameliorative effects of ellagic acid (EA) on oxidative stress induced by chlorpyrifos (CPF) in carp, Cyprinus carpio, were investigated. Fish were divided into six groups: C (no treatment), EA (100 mg kg fish−1), CPF-1 (0.040 mg L−1), CPF-2 (0.080 mg L−1), CPF-1 + EA, and CPF-2 + EA. CPF and EA were applied simultaneously for 14 days and, at the end of the study, liver, kidney, and gill samples were collected from fish. On the taken tissue samples, malondialdehyde (MDA) level and some antioxidant enzyme (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione-S-transferase (GST)) activities were evaluated. The results demonstrated statistically significant increases in the MDA levels of the CPF-1 and CPF-2 groups. On the other hand, the MDA levels were significantly decreased by EA administration. Also, CPF exposure caused statistically significant increases in the SOD and GST activities and statistically significant decreases in the CAT and GPx activities. However, treatment with EA reversed negative alterations in the SOD, CAT, GPx, and GST activities. Therefore, the results of this study results showed that simultaneous treatment with EA alleviates CPF-induced oxidative stress in fish.
AtıfScopus8WoS10OpenAlex10
İncele - MakaleAçık Erişim2021Scopus SJRQ1Ameliorative effect of Fennel (Foeniculum vulgare) essential oil on chlorpyrifos toxicity in Cyprinus carpio
Pala, Ayşegül; Serdar, Osman; Mişe Yonar, Serpil; Yonar, Muhammet Enis
Chlorpyrifos (CPF) is an organophosphate pesticide that is frequently and widely used to control both agricultural and domestic pests worldwide. In this study, the protective effect of Fennel (Foeniculum vulgare) essential oil (FEO) was investigated in carp (Cyprinus carpio) exposed to CPF. The fish were divided into six groups that one control group (no treatment) and five experimental groups (FEO (3ml/100g diet) group, CPF1 (0.023 mg/l) group CPF2 (0.046 mg/l) group, CPF1 (0.023 mg/l) plus FEO (3ml/100g diet) group, CPF2 (0.046 mg/l) plus FEO (3ml/100g diet) group). Blood and tissue (liver, kidney, gill, and brain) samples were taken from the fish at the end of 14 days of application. Hemoglobin (Hb) level, nitoblue tetrazolium (NBT) activity, and total immunoglobulin (TI) level were measured in blood samples of fish. Acetylcholinesterase (AChE) activity was determined in brain tissue while malondialdehyde (MDA) level, reduced glutathione (GSH) level, catalase (CAT), and glutathione peroxidase (GPx) activity were determined in liver, kidney, and gill tissues. The results showed that there was a significant decrease in Hb level, NBT activity, and TI levels in CPF-treated fish compared to the control group. In addition, increased in MDA levels and significant decreases in GSH level, AChE, CAT, and GPx activities were observed in CPF-treated groups. However, FEO-treated was showed a significant improvement in all parameters except AChE activity compared to CPF groups. These study findings showed that FEO could improve CPF-induced toxicity in C. carpio, except inhibition of AChE activity.
AtıfScopus6WoS6OpenAlex8İncele - MakaleAçık Erişim2019Scopus SJRQ3Effect of the organophosphate insecticide chlorpyrifos exposure on oxidative stress and quality of Salmo coruhensis spermatozoa
Kutluyer, Filiz; Kocabaş, Mehmet; Erişir, Mine; Benzer, Fulya
The use of pesticides has been increasing along with increasing farming activities and has caused deleterious environmental impacts. Non-target organisms in particular, including fish, are affected by pesticides. In this work, the impacts of Chlorpyrifos (CPF) on sperm oxidative stress markers and sperm motility were investigated in vitro. CPF concentrations were 0 μg/L (control), 5 μg/L, 10 μg/L and 15 μg/L. Lipid peroxidation [malondialdehyde (MDA)], nonenzymatic antioxidants [glutathione (GSH)] and enzymatic [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)] activities in sperm cells were examined for the determination of oxidative stress status. Our findings showed that motility and survival of sperm cells significantly decreased with exposure to Chlorpyrifos. Biochemical assays revealed that CAT activity and levels of MDA and, GSH increased in spermatozoa based on CPF concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to CPF exposure. It can be deduced that CPF has the potential to disrupt sperm quality and to cause to oxidative stress in sperm cells of S. coruhensis.
AtıfScopus15WoS14OpenAlex13İncele - MakaleAçık Erişim2018Scopus SJRQ3Effect of Tunceli garlic on some immunological parameters in Cyprinus carpio exposed to chlorpyrifos
Pala, Ayşegül; Şeker, Engin; Yonar, M. Enis
In this study, the effect of Tunceli garlic (Allium tuncelianium) on hemoglobin (Hb) level, oxidative radical production of neutrophils (Nitoblue tetrazolium assay-NBT activity) and total immunoglobulin (TI) content in carp (Cyprinus carpio) exposed to chlorpyrifos (CPF) was investigated. The 96 hour LC50 value of CPF on C. carpio was calculated to as 0.230 mg/L. The fishes were exposed to sublethal concentration of chlorpyrifos (1/8 of LC50 value: 0.029 mg/L), and Tunceli garlic (20 and 40 g/kg diet) was simultaneously administered for 21 days. Blood samples were taken from the fishes at 7, 14 and 21 days and analysed to determine the Hb levels, the NBT activity and the TI content. There was a significant decrease in the Hb level, the NBT activity and the TI content of CPF-treated fish. However, Tunceli garlic reversed the Hb level, the NBT activity and the TI content. In conclusion, this study demonstrated that CPF had a negative effect on the immunological values of the fish. The simultaneous administration of Tunceli garlic was neutralised CPF-induced toxicity.
AtıfScopus5WoS4OpenAlex6İncele