Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Ak, Kübra(1)
- Amir, Fachrurozi(1)
- Arslan, Zikri(1)
- Ates, Mehmet(1)
- Batubara, Agung S.(1)
- Camas, Mustafa(1)
- Demir, Veysel(1)
- Er, Akif(1)
- Fadli, Nur(1)
- Hasan, Bustari(1)
- Kaya, Hasan(1)
- Kocabas, Filiz Kutluyer(1)
- Marimuthu, Kasi(1)
- Minaz, Mert(1)
- Muchlisin, Zainal A.(1)
- Nur, Firman M.(1)
- Pala, Ayşegül(1)
- Serdar, Osman(1)
- Siti-Azizah, Mohd N.(1)
- Tang, Usman M.(1)
- Ulviye Karacalar(1)
- Wilkes, Martin(1)
- Yilmaz, Sevdan(1)
- Yonar, M. Enis(1)
- Şeker, Engin(1)
Yayınlar
- MakaleAçık Erişim2025Scopus SJRQ1Unlocking the potential of nutmeg oil: A sustainable alternative for rainbow trout anesthesia in aquaculture
Minaz, Mert; Er, Akif; Ak, Kübra; Serdar, Osman
In aquaculture, interest in natural anesthetic agents as alternatives to synthetic anesthetics has been steadily increasing. This is due to concerns over residue accumulation, environmental impact, and growing demand for sustainable practices. Within this context, the present study focused on the anesthetic effects of nutmeg essential oil at three different concentrations in rainbow trout (Oncorhynchus mykiss, 94.42 ± 23.9 g): low (400 µL/L), medium (600 µL/L), and high (800 µL/L). Induction and recovery times were recorded, and samples were collected at 0, 2, 4, and 8 hours post-anesthesia for antioxidant, cortisol, and hematological analyses. Additionally, gill tissues were sampled immediately after anesthesia for histological examination. While the induction time for the low concentration exceeded 3 minutes, the induction and recovery times for the other concentrations ranged within acceptable limits (3 min for induction and 5 min for recovery). Blood parameters exhibited an increasing trend until the 4th hour post-anesthesia, peaking at the 2nd hour, before returning to control levels by the 4th hour. Histological evaluation revealed healthy gill tissues in the control and low-concentration group (400 µL/L), whereas hyperplasia was observed in the medium (600 µL/L) and high-concentration (800 µL/L) groups. Antioxidant enzyme activity (superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and glutathione peroxidase (GSH-Px)) indicated potential oxidative stress at the high anesthetic concentration. Similarly, cortisol levels were higher at the high concentration, with plasma cortisol showing a slight initial increase followed by a return to normal levels over time. In conclusion, this study highlights that nutmeg oil at a concentration of 600 µL/L may serve as a promising natural alternative to synthetic anesthetics in aquaculture. However, future studies should include a synthetic anesthetic as a control to allow for direct comparisons and a more comprehensive evaluation of its efficacy and physiological effects.
AtıfScopus8WoS7OpenAlex7
İncele - Doktora TeziAçık Erişim2023Diyet takviyesi olarak kullanılan Eucalyptus globulus (okaliptüs ) ve Cinnamomum camphora (kafur) esansiyel yağlarının kültür çipura balıklarında (Sparus aurata) büyüme performansı, hematoloji, serum biyokimyasal ve antioksidan parametreler üzerine etkileri
Ulviye Karacalar
Biyoaktif bileşikleri nedeniyle esansiyel yağlar, sürdürülebilir su ürünleri yetiştiriciliğinde antibiyotiklere doğal bir alternatif olarak kabul edilebilir. Okaliptüs (Eucalyptus globulus) ve kafur (Cinnamomum camphora) esansiyel yağları, farmasötik özellikleriyle yaygın olarak bilinirler, ancak bu esansiyel yağların su ürünleri yetiştiriciliğinde kullanımını araştıran çalışmalar azdır. Bu çalışma, yavru çipura balığı (Sparus aurata) diyetine, farklı dozlarda uçucu yağlardan Eucalyptus globulus ve Cinnamomum camphora ilavesinin, büyüme performansı, hematolojik, biyokimyasal ve immünolojik parametreler üzerindeki etkilerini değerlendirmek için yapılmıştır. Bu amaçla balıklar (Ağırlık 14.62 ± 0.06 g; Boy 10.64 ± 0.20 cm) 60 gün boyunca, Eucalyptus globulus ve Cinnamomum camphora için farklı gruplara ayrılan tanklarda, her biri kendi grubu içinde, canlı ağırlıklarının % 2'si oranında 3 tekrarlı olarak, % 0 (kontrol), % 0.5, % 1 ve % 2 oranlarında 4 farklı konsantrasyonda esansiyel yağ içeren yemlerle beslenmiştir. Sonuçlar, nihai ağırlık, ağırlık artışı, günlük büyüme ve spesifik büyüme hızının, kontrol grubuna kıyasla tüm deney gruplarında önemli ölçüde arttığını göstermiştir. Yemden yararlanma oranı, % 1 ve % 2 Cinnamomum camphora uygulamasında kontrol grubu ile aynı seyrederken, % 2 oranındaki Eucalyptus globulus ilaveli diyet uygulamasında kontrol grubuna oranla belli bir artış gösterdiği görülmüştür. Diyetle alınan Eucalyptus globulus ve Cinnamomum camphora takviyeleri, kontrol grubuyla karşılaştırıldığında WBC (Beyaz kan hücresi) sayısını ve NEU(nötrofil) seviyesini önemli ölçüde arttırmıştır. Denemede kolesterol artışı, % 2 Cinnamomum camphora ve % 1 Eucalyptus globulus gruplarında, trigliserit oranı ise en yüksek % 1 Cinnamomum camphora grubunda görülmüştür. Deney sonunda kolesterol, trigliserit, glukoz, ALT (Alanin aminotransferaz) ve AST (Aspartat aminotransferaz) değerlerinde de istatistiki olarak önemli bir fark gözlemlenmiştir. Sonuç olarak bu çalışma her iki esansiyel yağ takviyesinin de yavru çipura balıklarında büyüme performansı, bağışıklık, hematolojik ve biyokimyasal parametreleri iyileştirdiğini göstermiştir.
- MakaleYalnız künye2022Scopus SJRQ2Effect of increasing water temperature on the physiology and gill histology of Barramundi, Lates calcarifer (Pisces, Perciformes) fingerlings
Amir, Fachrurozi; Muchlisin, Zainal A.; Nur, Firman M.; Fadli, Nur; Siti-Azizah, Mohd N.; Wilkes, Martin; Tang, Usman M.; Hasan, Bustari; Batubara, Agung S.; Kocabas, Filiz Kutluyer
Barramundi, Lates calcarifer is one of the commercial fish in Indonesia and other Southeast Asian countries. The species inhabits the coastal waters and is vulnerable to changes in the terrestrial environment due to coastal degradation, pollution, and global warming. The increase in temperature as a consequence of global warming affects the distribution and survival of aquatic organisms including fish. Therefore, this study aims to examine the effect of increasing water temperatures on the physiological conditions and gill histology of barramundi. The fish were exposed to eight levels of water temperature, ranging from 28oC to 35oC, for 15 days. The initial temperature was 28oC, which increased gradually by 1oC per 12-hour. Blood samples were taken at the end of the experiment for hemoglobin, glucose, cortisol, erythrocytes, leucocytes, and blood abnormality analyses. Gill samples were also taken for histological analysis. The results showed that water temperature significantly affected the level of glucose, cortisol, and erythrocyte cells (P<0.05), but did not influence hemoglobin levels and survival rate (P>0.05). A rapid increase in glucose and cortisol levels was also discovered at a temperature of 31oC above. The highest erythrocyte cell count was found at 35oC, gill cell abnormalities were occurred at temperature of 31oC and above, and the blood abnormalities increased linearly with water temperature. Based on these results, it is concluded that the water temperature did not affect the survival rate but the increase in temperature causes intense physiological stress.
AtıfScopus9İncele - MakaleAçık Erişim2018Scopus SJRQ3Effect of Tunceli garlic on some immunological parameters in Cyprinus carpio exposed to chlorpyrifos
Pala, Ayşegül; Şeker, Engin; Yonar, M. Enis
In this study, the effect of Tunceli garlic (Allium tuncelianium) on hemoglobin (Hb) level, oxidative radical production of neutrophils (Nitoblue tetrazolium assay-NBT activity) and total immunoglobulin (TI) content in carp (Cyprinus carpio) exposed to chlorpyrifos (CPF) was investigated. The 96 hour LC50 value of CPF on C. carpio was calculated to as 0.230 mg/L. The fishes were exposed to sublethal concentration of chlorpyrifos (1/8 of LC50 value: 0.029 mg/L), and Tunceli garlic (20 and 40 g/kg diet) was simultaneously administered for 21 days. Blood samples were taken from the fishes at 7, 14 and 21 days and analysed to determine the Hb levels, the NBT activity and the TI content. There was a significant decrease in the Hb level, the NBT activity and the TI content of CPF-treated fish. However, Tunceli garlic reversed the Hb level, the NBT activity and the TI content. In conclusion, this study demonstrated that CPF had a negative effect on the immunological values of the fish. The simultaneous administration of Tunceli garlic was neutralised CPF-induced toxicity.
AtıfScopus5WoS4OpenAlex6
İncele - MakaleAçık Erişim2016Scopus SJRQ1Chronic exposure of tilapia (Oreochromis niloticus) to iron oxide nanoparticles: Effects of particle morphology on accumulation, elimination, hematology and immune responses
Ates, Mehmet; Demir, Veysel; Arslan, Zikri; Kaya, Hasan; Yilmaz, Sevdan; Camas, Mustafa
Effects of chronic exposure to alpha and gamma iron oxide nanoparticles (alpha-Fe2O3 and gamma-Fe2O3 NPs) were investigated through exposure of tilapia (Oreochromis niloticus) to 0.1, 0.5 and 1.0 mg/L (9.2 x 10(-4), 4.6 x 10(-3) and 9.2 x 10(-3) mM) aqueous suspensions for 60 days. Fish were then transferred to NP-free freshwater and allowed to eliminate ingested NPs for 30 days. The organs, including gills, liver, kidney, intestine, brain, spleen, and muscle tissue of the fish were analyzed to determine the accumulation, physiological distribution and elimination of the Fe2O3 NPs. Largest accumulation occurred in spleen followed by intestine, kidney, liver, gills, brain and muscle tissue. Fish exposed to gamma-Fe2O3 NPs possessed significantly higher Fe in all organs. Accumulation in spleen was fast and independent of NP concentration reaching to maximum levels by the end of the first sampling period (30th day). Dissolved Fe levels in water were very negligible ranging at 4-6 mu g/L for alpha-Fe2O3 and 17-21 mu g/L for gamma-Fe2O3 NPs (for 1 mg/L suspensions). Despite that, Fe levels in gills and brain reflect more dissolved Fe accumulation from metastable gamma-Fe2O3 polymorph. Ingested NPs cleared from the organs completely within 30-day elimination period, except the liver and spleen. Liver contained about 31% of alpha- and 46% of gamma-Fe2O3, while spleen retained about 62% of alpha- and 35% of the gamma-polymorph. No significant disturbances were observed in hematological parameters, including hemoglobin, hematocrit, red blood cell and white blood cell counts (p > 0.05). Serum glucose (GLU) levels decreased in treatments exposed to 1.0 mg/L of gamma-Fe2O3 NPs at day 30 (p < 0.05). In contrast, GLU levels increased during the elimination period for 1.0 mg/L alpha-Fe2O3 NPs treatments (p < 0.05). Transient increases occurred in glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and lactate dehydrogenase (LDH). Serum Fe levels did not change during exposure (p > 0.05), but increased significantly within elimination period due to mobilization of ingested NPs from liver and spleen to blood. Though respiratory burst activity was not affected (p > 0.05), lysozyme activity (LA) was suppressed suggesting an immunosuppressive effects from both Fe2O3 NPs (p < 0.05). In contrast, myeloperoxidase (MPO) levels increased significantly in treatments exposed to alpha-Fe2O3 NPs (p < 0.05), and the effect from gamma-polymorph was marginal (p > 0.05). The results indicate that morphological differences of Fe2O3 NPs could induce differential uptake, assimilation and immunotoxic effects on O. niloticus under chronic exposure. (C) 2016 Elsevier B.V. All rights reserved.
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