Sürdürülebilir Kalkınma Amaçları

• 3

Yazarlar

• Acar, Mikail(1)
• Ak, Gunes(1)
• Avşar, Ömer Kaan(1)
• Caprioli, Giovanni(1)
• Diken, Mehmet Emin(1)
• Dogan, Serap(1)
• Karasakal, Ömer Faruk(1)
• Kasbolat, Sevgi(1)
• Kirici, Muammer(1)
• Mişe Yonar, Serpil(1)
• Nilofar(1)
• Paksoy, Mehmet Yavuz(1)
• Ponniya, Sathish Kumar M.(1)
• Poustforoosh, Alireza(1)
• Santanatoglia, Agnese(1)
• Tüzün, Burak(1)
• Uba, Abdullahi Ibrahim(1)
• Uysal, Ahmet(1)
• Wild Korkmaz, Franziska Johanna(1)
• Yilmaz Kardas, Begumhan(1)
• Yonar, Muhammet Enis(1)
• Zengin, Gokhan(1)
• İspir, Ünal(1)
• Şeker, Engin(1)

• MakaleAçık Erişim2026Scopus SJRQ2/r/munzur/1181
  Change of micronucleus frequencies, antioxidant parameters and in silico in carp (Cyprinus carpio) fry exposed to ibuprofen

  Kirici, Muammer; Tüzün, Burak; Yonar, Muhammet Enis; Poustforoosh, Alireza; Mişe Yonar, Serpil; Şeker, Engin; İspir, Ünal

  Ibuprofen, a non-steroidal anti-inflammatory drug, has been detected in the aquatic environment, but little is known about its effects on fish and other aquatic organisms. This study aimed to investigate the effect of ibuprofen on micronucleus levels in erythrocytes and whole-body antioxidant parameters of carp (Cyprinus carpio) fry. Fish were exposed to immersion in two different doses of ibuprofen concentrations (50 and 250 μg/L) for 24, 48, 72 and 96 h and 14 days. No mortalities occurred in any group during the test. Abnormal behaviours such as irregular swimming, loss of balance and hanging vertically in the water were observed in some fish exposed to acute toxicity. In this study, a significant increase in the micronucleus number of red blood cells was observed throughout the study period (p < 0.05). Antioxidant parameters were measured spectrophotometrically. Results obtained showed that ibuprofen significantly increased (p < 0.05) malondialdehyde (MDA) levels, glutathione S-transferase (GST) and catalase (CAT) activities, but decreased (p < 0.05) the glutathione (GSH) levels of whole-body carp fry in a dose-dependent manner. Furthermore, ibuprofen significantly increased MDA, GST and CAT levels, but increased the GSH levels of whole-body carp fry in a time-dependent manner. These results indicate that carp fry is sensible to human pharmaceutical agents in aquatic environments. The manner and the constructed interactions between ibuprofen and two protein targets (immunoglobin and haemoglobin) were investigated using molecular docking and molecular dynamics simulation. The results indicated that ibuprofen could affect the haemoglobin activity. Finally, absorption, distribution, metabolism, excretion and toxicity analysis was performed to examine the drug potential of the studied molecules.

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  AtıfScopus3·OpenAlex5

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• MakaleAçık Erişim2025Scopus SJRQ2/r/munzur/689
  In vitro and in silico analyses of cytoprotective, antigenotoxic and antimutagenic potential of Phlomis pungens var. hirta extracts against oxidative damage

  Wild Korkmaz, Franziska Johanna; Dogan, Serap; Yilmaz Kardas, Begumhan; Diken, Mehmet Emin; Karasakal, Ömer Faruk; Acar, Mikail

  Phlomis pungens var. hirta Velen has been used by local people for food and to treat diseases but there are limited number of investigations in literature about this plant and cyto/genotoxicity analyses have not been done before. Hence, this study aimed to find out cell-protective activity and DNA damage prevention ability of P. pungens var. hirta by MTS, micronucleus, 8-OHdG and somatic mutation recombination tests (SMART). In addition, liquid chromatography, antioxidant/antibacterial activity analyses and in silico biological activity profiling were performed. The healthy lymphocyte cell growth was supported by the plant extracts and none of the extracts was genotoxic. 8-OHdG test showed that methanolic and ethanolic extracts of the plant were able to decrease the oxidative DNA damage. According to the SMART results, extracts could suppress the genotoxic effects of an important environmental pollutant, H2O2. Rutin hydrate and quercetin were found in highest concentrations and the extracts could be characterized as antioxidant/antibacterial. In silico analysis also showed that the major compounds of this plant can be considered as chemoprotective. This is the first study that assessed the cell protective activity and DNA damage prevention capacity of P. pungens var. hirta, which should be regarded as a potential source for nutritional supplements and therapeutic products.

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  AtıfOpenAlex1

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• MakaleAçık Erişim2024Scopus SJRQ1/r/munzur/2088
  Integrating chemical analysis with in vitro, in silico, and network pharmacology to discover potential functional compounds from Marrubium astracanicum subsp. macrodon

  Avşar, Ömer Kaan; Kasbolat, Sevgi; Ak, Gunes; Nilofar; Caprioli, Giovanni; Santanatoglia, Agnese; Uysal, Ahmet; Uba, Abdullahi Ibrahim; Ponniya, Sathish Kumar M.; Paksoy, Mehmet Yavuz

  The members of the genus Marrubium are of great interest because they contain biologically active compounds. With in this mind, we aimed to examine the chemical profiles and biological activities (antioxidant, enzyme inhibition and antimicrobial effects) of different extracts of Marrubium astracanicum subsp. macrodon. Extractions of the plants were carried out with the solvent n-hexane, acetone, acetone/water (70 %), and water. The antioxidant activity of these extracts was evaluated through six different assays, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), cupric reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), metal chelating ability (MCA), and phosphomolybdenum (PBD). Additionally, the inhibitory effects of these solvents on five enzymes acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, amylase, and glucosidase were investigated. The study detected 38 compounds in a plant, with the highest content in acetone and acetone/water extracts. The acetone/water extract demonstrated the highest antioxidant activity aligning with its superior total phenolic (51.9 mg gallic acid equivalent (GAE)/g) and flavonoid (31.0 mg rutin equivalent (RE)/g) content, highlighting a direct correlation between bioactive compound concentration and antioxidant potential across various assays. Regarding the enzyme inhibition activity, the acetone/water extract of this plant exhibited significant AChE inhibition measuring 2.5 mg galantamine equivalent (GALAE)/g and anti-glucosidase activity measuring 1.2 mmol acarbose equivalent (ACAE)/g. In antimicrobial evaluation, the acetone extract exhibited good antimicrobial activity against B. cereus at 0.2 mg/ml dose. In molecular docking analysis, delphinidin 3,5 diglucoside showed a good interaction with the active site of AChE active. Through the application of network pharmacology, an in-depth exploration was conducted to unravel the potential of compounds from M. astracanicum against Alzheimer's disease. The study specifically focused on identifying key targets within the Alzheimer's disease pathway, pinpointing crucial proteins such as Glycogen synthase kinase-3 (GSK3), Prostaglandin-endoperoxide synthase 2 (PTGS2), and Alzheimer's-associated amyloid precursor protein (APP). In summary, the plant can serve as a valuable ingredient in the production of functional products within the pharmaceutical, nutraceutical, and cosmeceutical applications.

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  AtıfScopus4·OpenAlex4

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