Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Zengin, Gokhan(5)
- Ak, Gunes(3)
- Mahomoodally, Mohamad Fawzi(3)
- Benzer, Fulya(2)
- Cakilcioglu, Ugur(2)
- Kandemir, F. M.(2)
- Pala, Ayşegül(2)
- Sadeer, Nabeelah Bibi(2)
- Sinan, Kouadio Ibrahime(2)
- Çakılcıoğlu, Uğur(2)
- Adigüzel, Ragip(1)
- Aktumsek, Abdurrahman(1)
- Alan, Yusuf(1)
- Angeloni, Simone(1)
- Anıl Polat(1)
- Babacan, Ebru Yuce(1)
- Babacan, Ebru Yüce(1)
- Bahşi, Muammer(1)
- Balos, Maruf(1)
- Barim-Oz, O.(1)
- Benzer, E.(1)
- Benzer, F.(1)
- Bouyahya, Abdelhakim(1)
- Buldurun, Kenan(1)
- Cakli, Sukran(1)
- Cakır, Oguz(1)
- Caprioli, Giovanni(1)
- Cetiz, Mehmet Veysi(1)
- Ceylan, Ramazan(1)
- Costa, Emmanoel V.(1)
- Custódio, Luísa(1)
- Cziáky, Zoltán(1)
- Darendelioglu, Ekrem(1)
- Erdem, Ömer Alper(1)
- Erişir, Mine(1)
- Erol, Nida Demirtas(1)
- Esra Yüksel(1)
- Fatih Efendioğlu(1)
- Fernández-Ochoa, Álvaro(1)
- Gallo, Monica(1)
Yayınlar
- MakaleAçık Erişim2025Scopus SJRQ4Effects of chlorpyrifos on the freshwater mussel Unio elongatulus eucirrus (Bourguignat, 1860), with a focus on neurotoxicity and oxidative stress responses; [Efectos del clorpirifos en el mejillón de agua dulce Unio elongatulus eucirrus (Bourguignat, 1860), con especial atención a la neurotoxicidad y las respuestas al estrés oxidativo]
Zorlar, Faruk; Şeker, Engin; Pala, Ayşegül
This study set out to investigate the effects of the organophosphate insecticide chlorpyrifos (CPF) on certain biochemical parameters of the freshwater mussel Unio elongatulus eucirrus. Mussels were exposed to varying concentrations of CPF (10, 20, and 40 μg·L-1) for duration of 96 hours. Samples were collected from the mussels at the 24th and 96th hours of the experiment. The results indicated that exposure to CPF concentrations led to a decline in the activities of acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities and, accompanied by an increase in malondialdehyde (MDA), and decreased reduced glutathione (GSH) levels in mussels. In conclusion, the present study demonstrates that CPF caused AChE inhibition, formation of oxidative stress, and negative effects on certain antioxidant parameters in the freshwater mussel U. elongatulus eucirrus.
- MakaleAçık Erişim2025Scopus SJRQ2Chemical Characterization, Antioxidant and Enzyme-Inhibitory Activities of Different Extracts from Three Phlomis Species
Yagi, Sakina; Zengin, Gokhan; Cetiz, Mehmet Veysi; Cziáky, Zoltán; Jeko, József; Bahşi, Muammer; Çakılcıoğlu, Uğur; Turumtay, Halbay; Aktumsek, Abdurrahman
Phlomis species (family Lamiaceae) are highly valued as food and herbal medicine. The present study is designed to investigate the chemical composition and antioxidant and enzyme inhibitory activities of extracts from P. fruticosa, P. herba-venti, and P. kurdica aerial parts. Different classes of metabolites, including phenolic acids, phenylethanoids, flavonoids, iridoids, organic acids, terpenes, and fatty acids, are identified in the three species, with methanol as the best solvent to recover bioactive compounds from the three species in addition to ethyl acetate for P. kurdica. Around 70% methanol extract of P. herba-venti exerts the best radical scavenging and ions-reducing properties, while its methanol extract exhibits the highest acetylcholinesterase inhibitory activity. The ethyl acetate extract of P. fruticosa displays the best chelating power, and its other polar extracts have the highest total antioxidant activity. Furthermore, molecular docking and molecular dynamics simulations have underscored the therapeutic potential of bioactive compounds, including isoverbascoside, samioside, forsythoside B, and hattushoside. In conclusion, the study indicates that these three Phlomis species are a rich source of bioactive molecules with possible therapeutic applications, and the selection of appropriate extraction solvents is crucial for the targeted biological activity.
AtıfScopus23OpenAlex25
İncele - MakaleAçık Erişim2025Scopus SJRQ3Bioactive Compounds and Antioxidant Properties of Mucus from Freshwater Snails Viviparus contectus and Planorbis rubrum
Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Kızak, Volkan; Korkmaz, Büşra; Benzer, Fulya; Erişir, Mine
Abstract: As a viscous-elastic substance, snail mucus has a great attention in the medical and cosmetic industries in recent years due to contain many bioactive compounds. Freshwater snails are cosmopolite aquatic invertebrates and distribute nearly all types of inland aquatic bodies due to their adaptive abilities, rapid growth and short life cycles and, the compounds of mucus derived from freshwater snails has not been well documented. Hence, this study focused on identification bio-active ingredients and determination of protein content and antioxidant enzyme activities in freshwater snails Viviparus contectus and Planorbis rubrum mucus. Bioactive constituents of mucus in freshwater snails were characterized by Gas Chromatography-Mass Spectrometry (GC-FID-MS) analysis. Antioxidants (GSH, SOD, CAT and GSH-Px) and protein content were assessed in freshwater snails. Our data revealed that thirteen compounds are characterized with nonadecanoic acid methyl ester (11.57%), nonadecane (5.01%) and heptadecanoic acid methyl ester (4.19%) as the main components of P. rubrum mucus while eleven compounds are characterized with farnesol (11.23%), 5-hydroxymethyl-1,1,4α-Trimethyl-6-methylene-decahydronphthalen-2-ol (9.73%), kaur-16-en-18-oic acid methyl ester (9.64%), tricosane (9.62%), and kaurene (8.44%) as the main components of V. contectus mucus. Nonadecanoic acid methyl ester and nonadecane, known as effective bioactive constituents having antimutagenic and antitumor capability, were determined with 11.57 and 5.01% of the total bio-active composition of P. rubrum mucus, respectively. As a major constituent, farnesol was detected with 11.23% of the total bio-active composition of V. contectus mucus. Moreover, the presence of kaurene, known as a potent bioactive compound for cancer treatment, in notably quantity in V. contectus mucus. In addition, GSH levels and SOD, CAT, and GPx activities was highest level in P. rubrum compared to V. contectus. Protein content was highest level in V. contectus than P. rubrum. Overall, individual compounds of V. contectus and P. rubrum mucus could be isolated and purified and used for the treatment of a variety of human ailments.
- MakaleAçık Erişim2024Scopus SJRQ2Cytotoxic, antioxidant, and enzyme inhibitory activities of Centaurea stapfiana extracts and their HPLC-ESI-QTOF-MS profiles: Insights into an unexplored Centaurea species
Zengin, Gokhan; Fernández-Ochoa, Álvaro; de la Luz Cádiz-Gurrea, María; Jiménez, Francisco-Javier Leyva; Uba, Abdullahi Ibrahim; Ak, Gunes; Soomro, Sanam Iram; Balos, Maruf; Cakilcioglu, Ugur; Rodrigues, Maria João
The members of the genus Centaurea have a great interest in pharmaceutical and nutraceutical fields due to their biological potential. Based on this information, we aimed to evaluate the biological properties (antioxidant, enzyme inhibition and cytotoxicity) and chemical profile of the extract of Centaurea stapfiana, an unstudied species. The highest total phenolic content was found in the ethanol/water extract with 32.17 mg GAE/g. A total of 102 of them were identified by HPLC-ESI-QTOF-MS analysis. These compounds were mainly hydroxybenzoic acid and hydroxycinnamic acid as well as flavonoids. In the antioxidant tests, the ethanol/water extract had the best free radical scavenging and reducing ability. However, in the enzyme inhibition test, the ethanol extract was the most active. The extracts were also tested on two tumour cell lines (RAW 264.7 and HepG2) and one non-tumour cell line (S17). The ethanol extract showed the promising effect on HepG2 (cell viability: 28.6 % at 50 g/ml). Furthermore, we examined the interactions between the compounds and enzymatic and cellular targets. A good interaction was found between quercetin-3-xylosyl-(1- > 6)-glucoside and iNOS. In summary, our results suggest that C. stapfiana can be considered as a versatile raw material for the development of health-promoting applications in the pharmaceutical and cosmeceutical fields.
AtıfScopus13OpenAlex14İncele - Yüksek Lisans TeziAçık Erişim2023Keban baraj gölü Pertek avlak sahasında bulunan midye populasyonlarının ekolojik özelliklerinin su altı dalış tekniği ile belirlenmesi
Zühal Taş
Sualtı dalış gözlem çalışmalarında en küçük boydaki midyelerin kıyıda 5-10 cm derinliklerde tutunabilecekleri nesnelerin üzerinde kümeler halinde oldukları görülmüştür. Kıyıdan derinliklere doğru gidildikçe de midyelerin boylarının büyüdüğü tespit edilmiştir. Midyelerin uygun tutunma yeri olan yerlerde direk güneş ışınlarına doğru durmadıkları, kayalık yerlerin yan kısımlarına yapışarak indirekt olarak faydalandıkları görülmüştür. Büyük midyelerin genelde ortalama 30 mm büyüklükte oldukları belirlenmişse de, 40 mm den daha büyük ve çok sayıda midye kabuklarına da rastlanılmıştır. Ancak bunlardan hiç birine canlı olarak rastlanılmamış, kümeler halinde ölü ve boş kabukları bulunmuştur. Midyelerin su zeminine göre yayılım gösterdikleri belirlenmiştir. Çıplak taban çamurundansa rahatça tutunabilecekleri kayalık bölgeleri tercih ettikleri görülmüştür. Çıplak kumlu tabanlarda ise bulabildikleri teneke kutu, şişe, plastik artıklar, halat, balık ağları ve hatta tekne tabanlarına kadar tutunup kümeler halinde yaşadıkları tespit edilmiştir. Gözlem yapılan ve midyelerin yaşamalarına uygun zemin yapılarının tamamen midyeler ile kaplı oldukları olduğu, en fazla canlı midye kümelerinin 0-8 m arasındaki derinliklerde olduğu belirlenmiştir. Diğer taraftan suyun daha berrak olduğu ve gün ışığının daha derinlere ulaşabildiği bölgelerde ise 15 m derinliklerde de canlı midye kümelerine bolca rastlanılmış ve canlı midyelerin bulunduğu derinlik sınır yaklaşık 18 m civarlarında kalmıştır. Midyelerin sifonlarının sürekli olarak açık bir şekilde suyu süzme işlemini yaptıkları görülmüştür. Çalışma bölgesinin büyük bölümünde aşırı sediment olduğu midyelerin üstünü kapladığı, midyelerin bu durumda sifonların normalden daha fazla uzatarak suyu süzme işlemini gerçekleştirdikleri görülmüştür. Midyelerin canlıları da substrat olarak kullandıkları kerevitlerin ve hareketli midyeler olan Unionidlerin de üzerlerinde kümeler halinde bulundukları tespit edilmiştir. Midyelerin ortalama uzunluklarının 21,025±3,02 mm, ortalama genişliklerinin 10,33±1,49 mm, kabuklu ağırlıklarının ortalama 9,45±4,29 g ve ortalama et ağırlıklarının 1,99±1,55 mg olduğu tespit edilmiştir. Midyelerin uzunluk ile ağırlıkları arasında R² = 0,749 doğrusal ve güçlü bir ilişki bulunmuştur. "b" değerlerine baktığımızda negatif allometrik büyüme gösterdiği tespit edilmiştir. Bu çalışmada kıyıya yakın bölgelerde daha berrak ve sedimantasyon az olduğu yerlerden alınan midyeler ile, sedimentin daha çok olduğu ve hatta midyelerin üzerinin sedimentle kaplandığı alanlardan alınanların antioksidan seviyelerindeki farkın araştırılması amaçlanmıştır.Yapılan analiz sonuçlarına bakıldığında sedimentin az olduğu bölgeden alınan midyelerde SOD, CAT ve MDA seviyelerinin sedimentle kaplanan midyelerden daha yüksek olduğu tespit edilmiştir. SOD ve CAT'ın daha yüksek olması sedimentin az olduğu bölgelerdeki midyelerde oksidatif stresin düşük olduğu anlamına gelmekteyse de MDA'nın yüksek olması bu sedimentte bu duruma etki eden nedenlere de bakılmasını gerektirmektedir.
- Yüksek Lisans TeziAçık Erişim2022Tunceli sarımsağı (Allium tuncelianum Kollman) masere yağlarının yoğun stoklanmış Astacus leptodactylus Eschscholtz, 1823'da antioksidan parametrelere etkisi
Fatih Efendioğlu
Bu çalışmada da Tunceli sarımsağının (Allium tuncelianum Kollman) yoğunluk stresi altındaki kerevitler üzerinde (Astacus leptodactylus) antioksidan etkisinin incelenmesi amaç edinilmiştir. Kas analiz sonuçlarında iki deneme grubuda birbirine çok yakın D1 23,176±0,112 U/ml ve D2 23,384±0,370 U/ml rakamsal değerlerde bulundu. Kontrol grubunun değeri ise 21,056±0,209 U/ml rakamsal olarak daha düşük seviyelerde bulundu. İki deneme grubu arasında istatistiksel bir farklılık bulunmazken (P>0,05), deneme grupları ile kontrol grubu arasında anlamlı bir fark vardı (P<0,05). Hepatopankreas analiz sonuçlarında da kasta elde edilen değerlerde olduğu gibi, deneme grupları arasında rakamsal olarak birbirine yakın (D1 30,804±0,110 ve D2 30,502±0,757U/ml), kontrol grubu daha düşük değerde (26,422±0,600 U/ml) tespit edildi. Gene kas grubunda olduğu gibi deneme grupları arasında istatistiki fark yoktu (P>0,05) fakat deneme grupları ile aralarındaki fark anlamlıydı (P<0,05). Katalaz (CAT) analizleri de kasda ve hepatopankreasda ayrı ayrı yapıldı. Kas analiz sonuçlarında deneme grupları sonuçlarının birbirine rakamsal olarak çok yakın olduğu (D1; 15,556±0,349 ve D2; 15,266±0,178 nmol/dk/m), kontrol grubu değerinin (16,268±0,722 nmol/dk/m) ise daha yüksek olduğu gözlemlendi. Bununla birlikte gruplar arasındaki istatistiksel olarak bir fark bulunmadı (P>0,05). Hepatopankreas analiz sonuçlarında da hem kontrol ve hemde deneme grupları rakamsal olarak birbirine çok yakın (K; 23,694±0,455 nmol/dk/m, D1; 23,296±0,383 nmol/dk/m ve D2; 22,822±0,419 nmol/dk/m) olarak bulundu ve aralarında istatistiksel olarak da bir fark tespit edilmedi (P>0,05). Glutatyon peroksidaz sonuçları SOD sonuçlarına benzer bir durum sergilemiştir. Kas analiz sonuçlarına bakıldığında, deneme gruplarının rakamsal olarak kontrol grubundan (K; 6,728±0,403 U/mg) daha yüksek ve birbirine çok yakın olduğu (D1; 8,560±0,167 U/mg ve D2; 8,950±0,086 U/mg) tespit edildi. Deneme grupları arasında istatistiksel olarak bir farka rastlanılmazken (P>0,05), kontrol grubu ile aralarındaki farkın anlamlı olduğu görüldü (P<0,05). Kas analizine benzer sonuçlar hepatopankreas sonuçlarında görüldü. Deneme grupları rakamsal olarak birbirine çok yakın (D1; 7,182±0,094 U/mg ve D2; 7,110±0,220 U/mg) ve kontrol grubu değerinden (K; 5,898±0,331 U/mg) yüksek oldukları tespit edildi. Dene grupları arasında istatistiki bir fark yoktu (P>0,05) ve deneme grupları kontrol grubundan istatistiksel olarak farklıydı (P<0,05). MDA kas analizlerine bakıldığında en düşük değerin D2 grubunda (2,088±0,089 µM) olduğu, onu D1 grubunun takip ettiği (2,434±0,110 µM) ve en yüksek değerin kontrol grubunda (3,376±0,213 µM) olduğu görüldü. Deneme grupları arasında istatistiksel olarak farkın olmadığı, control grubundan ise istatistiksel olarak farklı oldukları tespit edildi. Hepatopankreas sonuçlarında da kas grubuna benzer durum görüldü. En düşük MDA değeri D2 grubunda (4,354±0,143 µM), ikinci olarak D1 grubunda (4,696±0,192 µM) ve en yüksek değerin kontrol grubunda (6,768±0,112 µM) olduğu gözlemlendi. Deneme grupları arasında istatistiki olarak bir fark olmazken, deneme grupları ile kontrol grubu arasında istatistiki bir farkın olduğu saptandı. Anahtar Kelimeler: Tunceli sarımsağı, Allium tuncelianum, Astacus leptodactylus, antioksidan
- Yüksek Lisans TeziAçık Erişim2022Masere sarımsak (Allium sativum Limne) ve Tunceli sarımsağı (Allium tuncelianum Kollman) yağlarının yoğun stoklanmış sazan balıklarının (Cyprinus carpio) bazı kan ve antioksidan parametrelerine etkileri
Anıl Polat
Bu çalışmada da Tunceli sarımsağının (Allium tuncelianum Kollman) diğer sarımsaklara (Allium sativum L.) göre olabilecek farklı etkilerini tespit etmek için, yem katkı maddesi olarak verilen sarımsakların sazan balıklarının (Cyprinius carpio) kan ve antioksidan parametrelerine etkisinin incelenmesi amaç edinilmiştir. Balıkların ilk tanklara bırakıldıkları günden sonra her 15 günde bir ölçümler alınmıştır. Rakamsal olarak en fazla ağırlık artışının masere Tunceli sarımsağı katkılı yemlerle beslenen grupta olduğu (20g), ikinci olarak onu kontrol grubunun (17 g), üçüncü olarak masere sarımsak yağı katkılı yem grubunun (15 g) ve son olarak da ayçiçeği yağı katkılı kontrol grubunun (12 g) sırasıyla takip ettiği belirlenmiştir. Gruplar arasında ağırlık artışında rakamsal olarak farklılıklar gözlenmiş olmakla beraber, istatistiksel olarak bir fark tespit edilememiştir (P>0,05). FCR (yem dönüşüm oranı) değerlerinde de benzer şekilde 1.16 ile en yüksek değer Tunceli sarımsağı, 1,38 ile ikinci olarak kontrol, 1,67 ile üçüncü olarak sarımsak ve son olarak 2,13 ile ayçiçeği kontrol grubunda bulunmuştur. Kan analizleri sonuçlarında, deneme grupları arasında LYM %, MID %, MID %, HGB, MCV, MCH, RDW-SD ve MPV değerlerinde istatistiksel olarak bir fark tespit edilememiştir (P>0.05). WBC, LYM #, MID #, GRAN #, RBC, HCT, MCHC, RDW-CV, PLT, PDW, PCTY ve P-LCR değerlerinde ise istatistiksel olarak anlamlı bir farkın olduğu görülmüştür (P<0,05). Süperoksit Dismutaz (SOD) analiz sonuçları incelendiğinde, en yüksek değerin sarımsak grubunda 0,639±0,057 U/ml olduğu, bu grubu 0,527±0,024 U/ml değeri ile Tunceli sarımsağının ikinci sırada, 0,520±0,059 U/ml değeri ile kontrol ayçiçeği yağının üçüncü sırada ve en düşük 0,456±0,047 U/ml değeriyle de kontrol grubunun son sırada izlediği görüldü. Kontrol ayçiçeği grubu ile Tunceli sarımsak grubu arasında istatistiksel bir fark görülmezken (P>0,05), diğer gruplar arasında istatistiksel bir farkın olduğu belirlendi (P<0,05). Katalaz (CAT) analiz sonuçlarına bakıldığında, en yüksek değerin 0,758±0,055 nmol/dk/ml ile Tunceli sarımsağı grubunun olduğu, sarımsak grubunun 0,625±0,032 nmol/dk/ml değeri ile ikinci, kontrol ayçiçeği grubunun 0,609±0,031 nmol/dk/ml değeri ile üçüncü ve kontrol grubunun 0,453±0,015 nmol/dk/ml değeri ile son sırada yer aldığı tespit edildi. Ayçiçeği yağı kontrol grubu ile sarımsak grubu arasında istatistiksel bir fark ortaya çıkmazken (P>0,05), diğer gruplar arasında önemli bir farkın olduğu tespit edildi (P<0,05). Malondialdehit (MDA) analiz sonuçlarında ise en düşük değerin 0,770±0,017 µM ile Tunceli sarımsağında olduğu, onu 1,133±0,046 µM değeri ile sarımsak grubunun ikinci sırada, 1,190±0,025 µM değeriyle ayçiçeği kontrol grubunun üçüncü sırada ve 1,351±0,031 µM değeri ile de kontrol grubunun son sırada takip ettiği görüldü. Ayçiçeği kontrol grubu ile sarımsak grupları arasında istatistiksel bir fark bulunmaz iken (P>0,05), diğer tüm gruplar arasında istatistiksel olarak anlamlı bir fark olduğu gözlemlendi (P<0,05). Anahtar Kelimeler : Tunceli sarımsağı, Cyprinius carpio, FCR, kan analiz, antioksidan
- MakaleYalnız künye2022Scopus SJRQ2Detailed Chemical Characterization and Biological Propensities of Malabaila lasiocarpa Extracts: An Endemic Plant to Turkey
Zengin, Gokhan; Zheleva-Dimitrova, Dimitrina; Babacan, Ebru Yüce; Polat, Rıdvan; Çakılcıoğlu, Uğur; Sadeer, Nabeelah Bibi; Costa, Emmanoel V.; Mahomoodally, Mohamad Fawzi; Naviglio, Daniele; Gallo, Monica
This study focused on the biological evaluation and chemical characterization of Malabaila lasiocarpa Boiss. (M. lasiocarpa) (Family: Apiaceae). The phytochemical profile, antioxidant, enzyme inhibitory of the methanolic, aqueous, dichloromethane, hexane extracts were investigated. Based on UHPLC-HRMS analyses, a total of 101 peaks were annotated or identified for the first time in M. lasiocarpa extracts. They include hydroxybenzoic, hydroxycinnamic, acylquinic acids and their glycosides, C- and O-glycosyl and O-diglycosyl flavonoids. In addition, 10 simple mono- and disubstituted coumarins together with 10 furanocoumarins were tentatively annotated. The methanolic extract possessing the highest phenolic (24.36±0.60 mg gallic acid equivalent/g extract) and flavonoid (69.15±0.37 mg rutin equivalent/g extract) content also exhibited the strongest radical scavenging potential against 2,2-diphenyl-1 picrylhydrazyl (21.73±0.42 mg Trolox equivalent/g extract, respectively), and highest reducing capacity (57.81±0.97 and 28.00±0.40 mg Trolox equivalent/g extract, for cupric reducing antioxidant capacity and ferric reducing antioxidant power, respectively). The dichloromethane extract substantially depressed the tyrosinase (73.92±5.37 mg kojic acid equivalent/g extract), α-amylase (0.63±0.01 mmol acarbose equivalent/g extract) and α-glucosidase (0.69±0.02 mmol acarbose equivalent/g extract) enzymes. This study has produced critical scientific data on M. lasiocarpa which are potential contenders for the development of novel phyto-pharmaceuticals.
AtıfScopus6OpenAlex5İncele - MakaleAçık Erişim2022Scopus SJRQ1Effects of the BHA and basil essential oil on nutritional, chemical, and sensory characteristics of sunflower oil and sardine (Sardina pilchardus) fillets during repeated deep-frying
Erol, Nida Demirtas; Erdem, Ömer Alper; Yilmaz, Sebnem Tolasa; Kayalar, Hüsniye; Cakli, Sukran
This study was to evaluate the BHA and basil essential oil on the quality attributes of deep-fried sardine fillets and frying oil. The extent of lipid oxidation was monitored by measuring peroxide value (PV), thiobarbituric acid reactive substances (TBARS), TBA, and changes in free fatty acids. Sensory analysis and color values upon the frying period were also determined. PV, FFA, TBA, and TBARS values in fish fillets and frying oil have peaked and then decreased at the end of frying except for the control. BEO showed the highest sensory scores compared to others. As a result, it is not possible to make a clear judgment about the effect of the natural or synthetic antioxidants on all lipid oxidation products that may occur during frying both fish fillets and frying oil. This study suggests that secondary oxidation products in frying oil and the sensorial quality of fish fillets can be better controlled by using basil essential oil.
AtıfScopus25WoS23İncele - MakaleYalnız künye2021Scopus SJRQ3Enzyme inhibition and antioxidant functionality of eleven Inula species based on chemical components and chemometric insights
Ceylan, Ramazan; Zengin, Gokhan; Mahomoodally, Mohamad Fawzi; Sinan, Kouadio Ibrahime; Ak, Gunes; Jugreet, Sharmeen; Cakır, Oguz; Ouelbani, Rayene; Paksoy, Mehmet Yavuz; Yılmaz, Mustafa Abdullah
The members of the genus Inula have been widely used in traditional medicinal. In the present study, 11 Inula species were investigated for their phytochemical composition, antioxidant and enzyme inhibitory effects. While quinic acid was the principal compound in I. anatolica, I. britannica, I. inuloides, I. oculus-christi, I. peacockiana, I. sechmenii, I. thapsoides and I. viscidula extracts (22.43–42.02 mg/g dry extract (DE)), I. aucheriana and I. discoidea extracts contained rutin as their most abundant compound (23.28 and 79.10 mg/g extract, respectively). Interestingly, all the Inula extracts were active inhibitors of the five key clinical enzymes (acetylcholinesterase (3.56–5.13 mg galantamine equivalent (GALAE)/g), butyrylcholinesterase (1.49–7.34 mg GALAE/g), tyrosinase (112.31–122.13 mg kojic acid equivalent (KAE)/g), α-glucosidase (0.77–2.08 mmol acarbose equivalent (ACAE)/g) and α-amylase (0.73–0.90 mmol ACAE/g)). Furthermore, the extracts were observed to exhibit substantial antioxidant capacity (1,1-diphenyl-2-picrylhydrazyl (DPPH): 58.99–188.22 mg trolox equivalent (TE)/g); 2,2-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) (ABTS): 90.51–220.97 mg TE/g; cupric reducing antioxidant capacity (CUPRAC): 169.88–460.53 mg TE/g; ferric reducing antioxidant power (FRAP): 81.57–237.99 mg TE/g; metal chelating activity: 8.31–25.39 mg ethylenediamine tetraacetate equivalent (EDTAE)/g; phosphomolybdenum: 1.55–2.49 mmol TE/g). In conclusion, the Inula species studied herein showed promising pharmacological potentials justified by the presence of a panoply of bioactive compounds.
AtıfScopus60OpenAlex60İncele - MakaleYalnız künye2021Scopus SJRQ2Two Medicinal Plants (Alkanna trichophila and Convolvulus galaticus) from Turkey: Chemical Characterization and Biological Perspectives
Mahomoodally, Mohamad Fawzi; Zengin, Gokhan; Sinan, Kouadio Ibrahime; Ak, Gunes; Sadeer, Nabeelah Bibi; Angeloni, Simone; Mustafa, Ahmed M.; Caprioli, Giovanni; Maggi, Filippo; Cakilcioglu, Ugur
The aim of the present study was to quantify selected phenolic compounds, determine antioxidant activity and enzyme inhibitory effects of the aerial parts of Alkanna trichophylla Hub.-Mor. (A. trichophylla) and Convolvulus galaticus Rost.ex Choisy (C. galaticus) extracts prepared by homogenizer-assisted extraction (HAE), maceration (MAC) and infusion techniques. This is the first time such study has been designed to validate the phytochemical composition and bioactivity of these plants. Multivariate analysis was conducted on collected data. Rutin and caffeoylquinic acid derivatives were the most significant compounds in A. trichophylla and C. galaticus, respectively. The highest antioxidant activity of A. trichophylla was mostly exhibited by HAE/methanolic extracts as determined by DPPH, ABTS, FRAP (51.39, 112.70 and 145.73 mg TE/g, respectively) and phosphomolybdenum (2.05 mmol TE/g) assays. However, significant antioxidant activities varied within the extracts of C. galaticus. HAE/methanolic extract of A. trichophylla significantly depressed AChE (2.70 mg GALAE/g), BChE (5.53 mg GALAE/g) and tyrosinase (26.34 mg KAE/g) activities and that of C. galaticus inhibited AChE (2.04 mg GALAE/g), tyrosinase (31.25 mg KAE/g) and α-amylase (0.53 mmol ACAE/g) activities significantly. We concluded that HAE was the most efficient extraction technique as high yield of compounds and promising bioactivities were recorded from extracts prepared. Multivariate analysis showed that types of solvents influenced recovery of compounds and biological activities. This research study can be used as one methodological starting point for further investigation on these plants as all results are clearly promising and open the door to further research challenges such as cytotoxicity evaluation, molecular docking analysis, and more screening of pharmacological actions.
AtıfScopus12OpenAlex10İncele - MakaleAçık Erişim2020Scopus SJRQ1Effect of trichlorfon on some haematological and biochemical changes in Cyprinus carpio: The ameliorative effect of lycopene
Mişe Yonar, Serpil; Yonar, M. Enis; Pala, Ayşegül; Sağlam, Naim; Sakin, Fatih
Trichlorfon (TCF) is an antiparasitic agent used in aquaculture to treat external parasites and it belongs to the chemical class of organophosphates. This study was conducted to investigate the ameliorating effects of lycopene on TCF-induced toxicity in carp by evaluating haematological parameters and oxidant/antioxidant status. Fish were randomly divided into seven experimental groups. These groups were arranged as follows: control (no treatment) group, corn oil group, lycopene (10 mg kg−1) group, TCF-I (11 mg L−1) group, TCF-I (11 mg L−1) plus lycopene (10 mg kg−1) group, TCF-II (22 mg L−1) group and TCF-II (22 mg L−1) plus lycopene (10 mg kg−1) group. At the end of 14 days administration, blood and tissue (liver, kidney, and gill) samples were collected. Haematological changes [red blood cell (RBC) and white blood cell (WBC) count, haemoglobin (Hb) concentration, haematocrit (Ht) level, and erythrocyte indices: mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin concentration (MCHC)] were determined in the blood samples, while antioxidant parameters [(malondialdehyde (MDA) and reduced glutathione (GSH) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities] were evaluated in the liver, kidney and gill samples. The results demonstrated that all the haematological parameters were significantly decreased in the TCF-I and TCF-II groups when compared to the control group. Notable increases in the tissue MDA levels were observed in the TCF-treated groups. In contrast, significant decreases were observed in the tissue SOD, CAT and GSH-Px activities and GSH levels in the TCF-I and TCF-II groups. However, simultaneous treatment with lycopene provided a marked normalisation of the haematological and oxidant/antioxidant parameters compared with the TCF-I and TCF-II groups. The findings show that dietary lycopene supplementation might be helpful in abrogation of TCF toxicity by changing haematological conditions and antioxidant status in fish.
AtıfScopus34WoS28OpenAlex31İncele - Yüksek Lisans TeziAçık Erişim2018Tunceli ilinde yetişen sumak bitkisinin fitokimyasal bileşenlerinin incelenmesi
Esra Yüksel
Sumak Anacardieceae ailesine ait bir bitkidir. Bu çalışmada Tunceli'de doğal olarak yetişen sumak bitkisi 5 farklı ilçeden toplandı, yıkandıktan ve oda sıcaklığında kurutulduktan sonra analize kadar buzdolabında saklandı. Sumak örneklerinde pH, renk, kül miktarları, toplam fenolik madde içeriği, DPPH serbest radikali giderme kapasitesi, metal şelatlama kapasitesi, CUPRAC, indirgeme kuvveti, mineral madde içeriği, organik asitlerden; tartarik asit, malik asit ve sitrik asit, fenolik bileşiklerden; gallik asit, vanilik asit, kafeik asit, rutin, resveratrol, (-)- epikateşin ve (+)- kateşin hidrat içeriği belirlendi. Yapılan çalışmada toplam fenolik madde (TFM) miktarı 1929±63 mg GAE kg-1-231±10 mg GAE kg-1 aralığında bulundu. Bütün sumak örneklerinde TFM miktarı en yüksek asitlendirilmiş metanol ekstraktında, DPPH serbest radikali giderme kapasitesi ise asitlendirilmiş asetonitril ekstraktında olduğu görüldü. S5 nolu örneğin ise bütün ekstraktlarının metal şelatlama kapasitesi diğer örneklerden yüksek olduğu fakat EDTA'nın metal şelatlama kapasitesinden düşük olduğu belirlendi. CUPRAC'ın ise en yüksek 118.0±3.0 mg KAE kg-1 ile S5 nolu örneğin asitlendirilmiş metanol ekstraktında, en düşük 10.2±0.6 mg KAE kg-1 ile yine S5 nolu örneğin asitlendirilmiş asetonitril ekstratında olduğu tespit edildi. Bütün örneklerin indirgeme kuvvetleri ise sentetik antioksidanların indirgeme kuvvetlerinden düşük olduğu, ayrıca örneklerin mineral madde bakımından da zengin oldukları, baskın olan organik asidin malik asit, fenolik bileşiğin ise gallik asit olduğu tespit edildi.
- MakaleAçık Erişim2016Scopus SJRQ3The influence of dietary antioxidant on ovarian eggs and levels of vitamin E, C, A, astaxanthin, β-carotene and oxidative stres in tissues of Astacus leptodactylus (Eschscholtz)
Barim-Oz, O.; Sahin, H.
The experiment was conducted to determine the most effective antioxidant (among the vitamin E (VE), vitamin C (VC), vitamin A (VA), astaxanthine (AX), beta-carotene (beta C)) on the ovarian egg number and size, level of VE, VC, VA, AX, beta C and oxidative stress (as malondialdehyde (MDA)) in the hepatopancreas, ovarian, gills and muscle tissue during ovarian development of Astacus leptodactylus. One control (C) and five experimental diets (EE, EC, EA, EAX and E beta C) were prepared. The EE, EC, EA, EAX and E beta C groups were formed by added 150 mg kg-1 VE, 200 mg kg-1 VC, 240 mg kg-1 VA, 200 mg kg-1 AX and 200 mg kg-1 beta C to diet C, respectively. At the end of the experiment found that the dietary antioxidants increased ovarian egg number and size and reduced the level of MDA in the tissues. Ovarian egg number and size were highest in the EE and EAX diet groups in the comparison to control (p< 0.001). The level of MDA in the tissues was lowest in the EAX diet group in the comparison to control (p< 0.001). The highest levels of VE, VC, VA, AX and beta C were found in the hepatopancreas and ovarian compared with muscle and gills. The highest level of MDA also was determined in the ovarian according to other tissues. In conclusion, the VE and AX in broodstock diets were the most effective antioxidants on the ovarian egg number and size of A. leptodactylus.
AtıfWoS16OpenAlex53İncele - MakaleAçık Erişim2016Scopus SJRQ3The Effects of Dietary Antioxidants on the Arginase Activity and Nitric Oxide Level of Narrow-Clawed Turkish Crayfish (Astacus leptodactylus, Esch 1823) in Moulting Period
Benzer, Fulya; Özçelik, Mehtap; Yildirim, Nuran Cikcikoglu
The effects of dietary antioxidants (vitamine E, vitamine C, Astaxhantin, beta carotene) on arginase activity and nitric oxide levels of hepatopancreas and muscle of freshwater crayfish (Actacus leptodactylus) were investigated during moulting. 150 mg kg(-1) vitamine E (VE), 200 mg kg(-1) vitamine C (VC), 200 mg kg(-1) Astaxhantin and 200 mg kg(-1) beta carotene were added to experimental diets. A. leptodactylus with these diets were fed with 2% of their total weight daily, in a day during 75 days. Arginase activity was increased in muscle of crayfish fed the diets VC, VE, astaxanthin and beta karoten but in hepatopancreas arginase activity was increased with beta carotene when compared to control. NO levels in muscle were decreased when compared to control but this decrease was not statically significant. NO levels in hepatopancreas of crayfish fed the diets VC and astaxanthin were higher than control groups. Our results demonstrated that dietary antioxidants affect the arginase activity and nitric oxide level of freshwater crayfish (Astacus leptodactylus, Esch. 1823) during moulting.
AtıfWoS3OpenAlex3İncele - MakaleYalnız künye2016Scopus SJRQ4Synthesis and Chemical Structure Elucidation of Two Schiff Base Ligands, Their Iron(II) and Zinc(II) Complexes, and Antiradical, Antimicrobial, Antioxidant Properties
Turan, Nevin; Savci, Ahmet; Buldurun, Kenan; Alan, Yusuf; Adigüzel, Ragip
Fe(II) and Zn(II) complex series have been synthesized from Schiff base ligands derived from furan-2-carbaldehyde and thiophene-2-carbaldehyde. Elemental, infrared, ultra-violet- visible, NMR and thermal analysis, also mass spectroscopy and magnetic susceptibility measurements were performed to explain the structures of these synthesized ligands and their metal complexes. The formulas based on analytical, spectral, and magnetic studies, the complexes can be demonstrated as [M(L-1)(2)](H2O)(n), [M(L-2)(2)](H2O)(n), where M = Zn(II) and Fe(II). An octahedral geometry was suggested for the Zn(II) and Fe(II) complexes. The antioxidant, antiradical and antimicrobial properties of the Zn(II) and Fe(II) complexes were investigated using different in vitro assays. Some of the complexes showed reasonable amount of antioxidant and antimicrobial activity.
AtıfWoS19OpenAlex19İncele - MakaleYalnız künye2012Scopus SJRQ3Beneficial effects of oral administrations of grape seed extract on healing of surgically induced skin wounds in rabbits
Sagliyan, A.; Benzer, E.; Kandemir, F. M.; Gunay, C.; Han, M. C.; Ozkaraca, M.
This study examined the effects of orally given grape seed extract (GSE) on healing of surgically induced full thickness skin wounds in terms of clinical, histopathological and biochemical aspects. A total of 48 healthy. adult male New Zealand rabbits were randomly divided in 2 equal experimental and control groups: one week prior the surgery, 260 mg/kg grape seed extract was orally given for 20 days to the assay animals. A circular full thickness skin wound (2.5 cm in diameter) was made in the back midline of all rabbits under anaesthesia then, macroscopic and histopathological aspects as well as the tissue oxidant/antioxidant balance were determined on 6 rabbits from each group slaughtered at the 5th, 10th, 15th and 20th days post-surgery. In treated rabbits, it was clinically observed that the wound contraction was significantly amplified and the histological processes of re-epithelisation, granulation tissue development, collagen accumulation and angiogenesis were markedly hastened whereas the inflammatory reaction and the ulcer disappeared more rapidly than in the not-treated controls. Furthermore, the maximal skin MDA formation observed on the 10th day was significantly depressed and the antioxidant catalase (CAT) and glutathione peroxidase (GSH-Px) activities remained significantly elevated until the 15th day compared to the controls. These results demonstrate the beneficial effects of treatment with GSE on skin wound healing in rabbits.
AtıfWoS4İncele - MakaleYalnız künye2011Scopus SJRQ3Effect of Pomegranate Seed Extract on Free Radical Damage and Antioxidant Activity Under Cisplatin-Induced Oxidative Stress Conditions in Rabbit Testes
Benzer, F.; Kandemir, F. M.; Yildirim, N. C.; Ozan, S. T.
The aim of this study is to evaluate the protective effect of pomegranate seed extract on cisplatin-induced oxidative stress in rabbits testes. Rabbits were divided into three groups; (i) being control group, (ii) cisplatin group and (iii) cisplatin + pomegranate seed extract group. The level of malondialdehyde and glutathione, the activity of catalase, glutathione peroxidase, were measured in testes tissue of rabbits. Malondialdehyde levels were increased depending on cisplatin. In the case of cisplatin + pomegranate seed extract, malondialdehyde levels were found to be lower than cisplatin group (p < 0.05). In cisplatin group, catalase, glutathione peroxidase activity and glutathione levels were decreased when compared to control but in cisplatin + pomegranate seed extract group, catalase, glutathione peroxidase activity and glutathione levels were found higher than cisplatin group (p < 0.05). The pomegranate seed extract supplementation may play a protective role or decrease the side effects of cisplatin-induced testes toxicity from cancer medicines.
AtıfWoS5İncele - MakaleAçık Erişim2010Scopus SJRQ3Effects of cypermethrin on antioxidant status, oxidative stress biomarkers, behavior, and mortality in the freshwater mussel Unio elongatulus eucirrus
Köprücü, Kenan; Yonar, Serpil Mise; Seker, Engin
In this study, the effects of cypermethrin on antioxidant status, oxidative stress biomarkers, behavior, and mortality in the freshwater mussel Unto elongatulus eucirrus were examined. Cypermethrin was applied at concentrations of 5, 10, 20, 40, 80, and 160 mu g/L, for 1, 24, 48, 72, and 96 h. With increasing cypermethrin concentrations, the mussels showed significantly (P < 0.05) increased lipid peroxidation (MDA), which might be associated with decreased levels of reduced glutathione (GSH) and catalase (CAT) activity in the digestive glands and gills of the mussels. Negative correlations were observed between the MDA and the GSH and CAT levels after cypermethrin exposure, indicating a protective role of GSH and CAT against lipid peroxidation, and suggesting the use of these antioxidants as a potential biomarker of toxicity associated with contaminant exposure in freshwater mussels. In addition, at 5 mu g/L, cypermethrin had no effect on the duration of the active and rest periods. All higher concentrations caused inhibition of the filtration activity by reducing the active periods and lengthening the rest periods. The active periods shortened as the cypermethrin concentrations increased, the reduction being 90% of the control at 160 mu g/L. Rest periods were longest (237% longer than the control) at 160 mu g/L. The 48, 72, and 96 h LC50 values of cypermethrin for mussels were determined as 96.50, 77.96, and 59.20 mu g/L, respectively (P < 0.05). The results indicate that the insecticide cypermethrin has a harmful effect not only on nontarget aquatic arthropods and fish, but also on mollusks, although the sensitivity of mussels is less than that of fish. Being a general toxicant for aquatic life, cypermethrin should be used with great caution in agriculture to protect natural waters from contamination.
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