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- MakaleAçık Erişim2026Scopus SJRQ2Change of micronucleus frequencies, antioxidant parameters and in silico in carp (Cyprinus carpio) fry exposed to ibuprofen
Kirici, Muammer; Tüzün, Burak; Yonar, Muhammet Enis; Poustforoosh, Alireza; Mişe Yonar, Serpil; Şeker, Engin; İspir, Ünal
Ibuprofen, a non-steroidal anti-inflammatory drug, has been detected in the aquatic environment, but little is known about its effects on fish and other aquatic organisms. This study aimed to investigate the effect of ibuprofen on micronucleus levels in erythrocytes and whole-body antioxidant parameters of carp (Cyprinus carpio) fry. Fish were exposed to immersion in two different doses of ibuprofen concentrations (50 and 250 μg/L) for 24, 48, 72 and 96 h and 14 days. No mortalities occurred in any group during the test. Abnormal behaviours such as irregular swimming, loss of balance and hanging vertically in the water were observed in some fish exposed to acute toxicity. In this study, a significant increase in the micronucleus number of red blood cells was observed throughout the study period (p < 0.05). Antioxidant parameters were measured spectrophotometrically. Results obtained showed that ibuprofen significantly increased (p < 0.05) malondialdehyde (MDA) levels, glutathione S-transferase (GST) and catalase (CAT) activities, but decreased (p < 0.05) the glutathione (GSH) levels of whole-body carp fry in a dose-dependent manner. Furthermore, ibuprofen significantly increased MDA, GST and CAT levels, but increased the GSH levels of whole-body carp fry in a time-dependent manner. These results indicate that carp fry is sensible to human pharmaceutical agents in aquatic environments. The manner and the constructed interactions between ibuprofen and two protein targets (immunoglobin and haemoglobin) were investigated using molecular docking and molecular dynamics simulation. The results indicated that ibuprofen could affect the haemoglobin activity. Finally, absorption, distribution, metabolism, excretion and toxicity analysis was performed to examine the drug potential of the studied molecules.
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İncele - MakaleYalnız künye2022Scopus SJRQ2The use of Gammarus pulex as a model organism for ecotoxicological assessment of ibuprofen and propranolol at environmental relevant concentrations
Cikcikoglu Yildirim, Nuran; Serdar, Osman; Basaran, Senay
The aim of this study is to assess the toxicity of ibuprofen (IBU) and propranolol (PRO) drugs usingGammarus pulex as a model organism. Firstly, the 96 h LC50 values of IBU and PRO were determined and then three sublethal concentrations of the drugs were exposed to G. pulex. The activities of superoxide dismutase (SOD), catalase (CAT) and acetylcholinesterase (AChE) were evaluated. SOD activity decreased in G. pulex exposed to IBU and PRO compared to control. In all groups exposed to IBU, CAT activity increased at different concentrations at 24 and 96 h. In the groups exposed to different PRO concentrations, CAT activities increased after 24 h compared to the control group (p < 0.05). AChE activities increased in all application groups exposed to IBU for 96 hours (p < 0.05). In conclusion, exposure to IBU and PRO resulted in increased oxidative damage. PRO has been found to cause neurotoxicity.
AtıfScopus7WoS5OpenAlex7İncele