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  • MakaleAçık Erişim2025
    The Relationship between Copper Tolerance and Antibiotic Susceptibility of Vagococcus salmoninarum Isolated from Kılavuzlu Dam Lake

    Gokhan, Haci Bayram; Ispır, Ünal; Özcan, Mikail; Şeker, Engin

    The contamination of aquatic ecosystems by heavy metals has been identified as a significant environmental hazard for aquatic animal species, including fish. The objective of this study was to investigate the tolerance of Vagococcus salmoninarum bacteria to copper and the impact of copper exposure on antibiotic susceptibility. The bacterial isolates, comprising six strains, were obtained from rainbow trout reared in cage culture at Kılavuzlu Dam Lake. The initial assessment of antibiotic susceptibility was conducted using the Kirby-Bauer disc diffusion method, which was subsequently validated through the determination of minimum inhibitory concentration (MIC). The broth microdilution method was employed, with the bacterial isolates exposed to concentrations of copper sulphate ranging from 0.1 to 1 mM. The resistance to multiple antibiotics was altered to be significantly altered following exposure to copper sulphate. The minimum inhibitory concentration (MIC) value of copper was observed to be 0.4 mM against V. salmoninarum. It was noted that V. salmoninarum, which has been exposed to copper in its natural environment, has developed the capacity to withstand stress and has undergone a change in its physiological status.

    • SDG 3 · Sağlık ve Kaliteli Yaşam
    • SDG 6 · Temiz Su ve Sanitasyon
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  • MakaleAçık Erişim2022
    Green Synthesis of Gold Nanoparticles Using Aqueous Extract of Asphodelus Aestivus, Coating with Chitosan Biopolymer and Cytotoxicity Studies

    Ates, Mehmet; Yilmaz, Ersen; Erbay, Muhammed

    The green synthesis of gold nanoparticles (Au-NPs) was carried out by pouring the aqueous extract of East Anatolian origin Asphodelus aestivus plant onto aqueous gold metal ions and reducing them via single-step one- pot method. The absorption peak of the synthesized nanoparticles gave a maximum at 575 nm. All the X-ray diffraction peaks at 2θ = 38.25 , 44.46 , 64.64 and 77.20 that index to (111), (200), (220), and (311) planes verify the successful synthesis of Au-NPs. Mostly spherical shape particles showed a homogeneous distribution with size range 20±5 nm are measured using TEM. From the FTIR spectrum, the peaks are seems to be related to phenolic compounds, flavonoids, benzophenones, terpenoids and anthocyanins which assume that they could act as the reducing agents. The plant extraction, one-pot, single-step method used is environmentally safe without the role of synthetic materials which is highly potential in mild and green synthesis applications. The Au-NPs were coated with chitosan biopolymer in aquatic solution medium and verified by SEM. Then, cytotoxic investigations of the biosynthesized Au-NPs were carried out by HUVEC cells. Au-NPs were showed toxic effects on cell culture, even if in a small amount. However, chitosan biopolymer coating increased cell viability.

    AtıfOpenAlex3
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  • MakaleYalnız künye2022
    Effects of Maca Root Extract on Some Molecular Parameters of Kidney Tissue in Rats Fed a High-Fat Diet

    Erten, Fusun

    Maca (Lepidium meyenii) has been used as a dietary supplement in ethnomedicine for centuries.\rMaca has recently become a high-functional food globally, due to its numerous biological benefits.\rThe high-fat diet (HFD) is closely related to the occurrence of obesity. Obesity is a common cause\rfor chronic kidney disease. The aim of this study was to investigate the effects of maca on organic\ranion transporters-3 (OAT-3), organic cation transporter-1 (OCT-1), organic cation transporter-2\r(OCT-2), insulin receptor substrate-1(IRS-1), vascular adhesion molecule-1 (VCAM-1) and\rvascular endothelial growth factor (VEGF) protein levels. In the study, 28 Sprague-Dawley male\rrats were randomly allocated into four groups (n=7); i) Control group (fed with standard diet); ii)\rGroup fed with standard diet and supplemented with Maca (40mg/kg/day) group; iii) Group fed a\rHFD; iv) Group fed with Maca+HFD. After the 60-day of study, kidney OCT-1, OCT-2 and IRS-1\rprotein levels showed a significant increase in the HFD+Maca treated group compared to the HFD\rgroup (P<0.05). Renal OAT-3 level was found to be significantly lower in the HFD group compared\rto the control group (P<0.0001). In addition, a significant decrease in VCAM-1 and VEGF protein\rlevels was observed in the HFD+Maca group compared to the HFD group (P<0.0001). These\rresults show the protective and regulatory activity in the Maca's kidney tissue when both normally\rand the HFD diet is fed

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  • MakaleYalnız künye2022
    Effect of Lutein/Zeaxanthin Isomers on the Musclin Signaling Pathway

    Sahin, Kazim; Er, Besir; Erten, Fusun

    Lutein (L) and zeaxanthin (Zi) are plant origin carotenoids. They have anti-inflammatory and\rantioxidant effects. Musclin is a myokine induced by physical activity. The aim of this study was to\rinvestigate the effect of L/Zi supplementation on serum and skeletal muscle musclin levels in rats\rsubjected to physical exercise. For this purpose, the rats were divided into four groups, each\rconsisting of seven rats, as the control group, no exercise, L/Zi treated and no exercise group\r(L/Zi), exercise group (E), and L/Zi treated and exercise group (E + L/Zi treatment). L/Zi was\radministered daily for eight weeks as an oral supplement by gavage at a dose level of 100 mg/kg\rBW. When compared with control, E, L/Zi and E + L/Zi decreased muscle triglyceride level by\r30.8%, 28.3% and 57.4%, respectively and increased serum and muscle musclin levels by 58.03%,\r67.4%, 136.5% and 49.4%, 50.1%, 85.6% respectively. In conclusion, it was determined that\rskeletal muscle and systemic musclin levels were stimulated by L/Zi combination in rats subjected\rto chronic exercise

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  • Derleme MakaleYalnız künye2021
    Hydrogenosomes in the Anaerobic Fungi: Hydrogen-Producing Organelles

    Yazdıç, Ferit Can; Yazdıç, Fadime; Kar, Bülent; Ozkose, Emin; Ekinci, Mehmet Sait

    Fungi form a very diverse group in eukaryotes. Most of the fungi contain mitochondria and capable of oxidative phosphorylation. On the other hand, anaerobic Neocallimastigomycota fungi are in the gastrointestinal tract of many herbivorous mammals, they contain an organelle known as hydrogenosome instead of mitochondria. These organelles capable of being the key to adaptation to an anaerobic environment that is is unique. In this review, we wish to briefly summarize what is known about function, structure, biochemistry, genetics properties and inheritance of these important organelles.

    AtıfTR Dizin4
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  • MakaleAçık Erişim2021
    Investigation of Chrysin Effects on Lead Asetat-Induced Liver Damage in Rats

    Benzer, Fulya; Kandemir, Fatih Mehmet; Yildirim, Serkan; Coşkun, Pınar; Küçükler, Sefa

    This study was conducted to investigate the effects of chrysin (CH) on lead acetate (PbA) inducing liver injury. In this study, 35 male Sprague Dawley rats were divided into 5 groups as 7 rats in each group. Group 1 (Control): Saline was given orally. Group 2 (CH group): CH (50 mg kg-1 weight day-1 ) were orally given rats for 7 days. Group 3 (PbA group): PbA (30 mg kg-1 ) was orally given to rats at 30 mg kg-1 PbA for 7 days. Group 4 (PbA +CH 25 group): 25 mg/kg CH was orally given to rats for 7 days. In addition, 30 mg kg-1 PbA was given orally to rats for 7 days after 30 minutes of the application of the CH. Group 5 (PbA+CH 50 group): 50 mg kg-1 CH was orally given to rats for 7 days. Furthermore, 30 mg kg-1 PbA was given orally to rats for 7 days after 30 minutes of the application of the CH. The rats were euthanized under mild sevoflurane anesthesia and tissue and blood samples were taken 24 hours after the last application (8th day). AST, ALT and ALP in serum, MDA, GSH and NO levels and SOD, CAT, GPx and arginase enzyme activities in liver tissue were measured. Serum ALT, AST and ALP levels increased in the PbA group when compared with the control, and this increase was partially corrected with CH. Liver MDA and NO levels increased in the PbA administered groups while antioxidant enzyme activities and GSH levels decreased. Although, CH administration decreased the increased MDA and NO levels, it increased the decreased antioxidant enzyme activities and GSH levels. Histologically, it was determined that CH decreased the necrosis, hydropic degenerations and mononuclear cell infiltrations seen in the livers of rats treated with PbA. As a result, CH is effective in the reducing PbA damages in the liver.

    • SDG 3 · Sağlık ve Kaliteli Yaşam
    AtıfOpenAlex3TR Dizin3
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  • MakaleYalnız künye2021
    Risk assessment using a novel hybrid method: a case study at the biochemistry department

    Derse, Onur; Göçmen, Elifcan; Narli, Mufide

    Risk management has gained substantial attention for the health sector in recent years. Healthcare systems are forced to deal with risk assessment due to deadly accidents. Biochemistry department is one of the most critical units, in which occupational accidents should be controlled preventively. Thus, this paper aims at developing a risk assessment process to minimize the occupational accidents. Failure mode and effects analysis (FMEA) and 5x5 matrix (L Matrix) as most effective quantitative risk analysis methods, are developed for a biochemistry department of a hospital in Turkey to evaluate the risk types discussed in related works and discovered during this work. First, the decision makers’ risk evaluation based on historical data of accidents are adopted to risk management process. Then, with regard to scoring of risk factors such as occurrence, severity and detectability, risk priority numbers and classes are obtained and preventive measures for the high-risk failure modes are determined. Analytic Hierarchy Process (AHP) method has been applied for prioritizing these measures to reduce or eliminate these risk types. Considering the results obtained from the AHP method, a goal programming model is developed to minimize the risk prevention costs. Obtained results demonstrate the effectiveness of the proposed methods.

    AtıfTR Dizin3
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  • MakaleYalnız künye2020
    Effects of Taurine on the Levels of PPARγ, IRS-1, HSP-27, HSP-72 and Histopathological Changes in Kidney of the Diabetic Rats

    Tuzcu, Mehmet; Erten, Fusun; Gencoglu, Hasan

    The objective of this study was to investigate the effects of taurine on PPAR-γ, IRS-1, HSP-27, and HSP-72 levels along with the kidney histopathological changes in streptozotocin (STZ) induced diabetic rats. In the study, 40 Wistar albino male rats were divided into four groups. (i) Control group, (ii) Taurine, rats received taurine with drinking water (2%); (iii) STZ, rats were injected with STZ (60 mg/kg i.p. as single dose) and (iv) STZ+Taurine, rats received taurine and injected with STZ. After 8 weeks of the study, the Taurine group was found to be statistically indifferent to the control group in terms of PPAR-γ, IRS-1, HSP-27, and HSP-72 levels (P>0.05). However, PPAR-γ (P<0.01), IRS-1 and HSP-72 (P<0.001) levels decreased in the STZ group. HSP-27 (P<0.01) levels increased in the STZ group as compared with the control. On the other hand, there were histopathological changes in the STZ group. The intensity of histopathological changes induced by STZ was decreased in the STZ+Taurine group. As a result, the taurine supplementation may prevent and treat the kidney damage in diabetic rats

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  • MakaleAçık Erişim2020
    The Effect of N-Acetylcysteine on Oxidant/Antioxidant Status in Irradiated Rats

    Baykara, Murat; Özçelik, Mehtap; Erisir, Mine; Guler, Osman

    N-Acetylcysteine (NAC) is both a strong antioxidant and a glutathione precursor. The effect of NAC on the oxidant/antioxidant status of some tissues of the irradiated rats was investigated. Twenty one rats were assigned to 3 groups; the control group, the irradiation group, for which physiological saline solution was administered as i.p. for three days and then, 9 Gy gamma irradiation was applied, and 3rdirradiation + NAC group for which NAC was adminis-tered as i.p. for three days as 300 mg/kg body weight and then the same dose of irradiation was applied. Upon irradia-tion, the increase determined in malondialdehyde (MDA) was significant in the liver, kidney, and brain tissues of the rats (P˂0.05). While glutathione peroxidase (GSH-Px) activity decreased in all of the tissues and superoxide dismutase (SOD) activity only in the liver (P˂0.01), glutathione (GSH) levels significantly increased in the kidney and ovarium tissues (P˂0.001). While NAC administration returned the increased MDA levels in the kidney and brain as a result of irradiation to normal levels (P˂0.05), it was determined that it did not return the increased MDA levels in the liver tissue to the normal level (P˂0.001). While NAC addition led to a significant increase in GSH levels of the liver, heart, spleen, brain and ovarium tissues compared to both control and irradiation groups, it caused a significant decrease in the kid-ney tissue compared to irradiation group (P˂0.001). As a result of NAC addition, a significant decrease was determined in spleen GSH-Px activity, heart and ovarium SOD activity compared to control and irradiation groups (P˂0.05). It can be asserted that GSH increasing by the addition of NAC is the main antioxidant that has a role in decreasing oxidative stress occurring as a result of irradiation. In the examination of MDA values, it was found that the addition of NAC pro-tected the kidney and brain against the oxidative damage induced by irradiation but NAC addition could remain insuffi-cient for the liver.

    • SDG 3 · Sağlık ve Kaliteli Yaşam
    AtıfOpenAlex2
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  • MakaleAçık Erişim2020
    SARS CoV-2 nsp1 Mutasyonlarının Protein Yapıda Ortaya Çıkardığı Değişimler

    Akbulut, Ekrem; Kar, Bülent

    Şiddetli akut solunum yolu sendromu koronavirüsü 2 (SARS CoV-2) pozitif polariteli ve tek iplikli bir RNA virüsüdür. Virüsün sebep olduğu COVID19 hastalığı on ay gibi kısa bir sürede 900 binden fazla insanın ölümüne neden oldu. Virüs ile mücadelede etkin ve spesifik bir ilaç ve aşı henüz bulunmamaktadır. İlaç ve aşı geliştirme çalışmaları virüsün yapısal ve fonksiyonel özelliklerinin kapsamlı bir şekilde anlaşılmasını gerekli kılmaktadır. Hızlı yayılım gösteren virüsün yüksek mutasyon hızı geliştirilecek aşı ve ilaçların etkinliklerini sürdürebilmelerinin önündeki en büyük engellerden biridir. Hücresel boyutta viral enfeksiyonun başlangıcında yer alan SARS CoV-2 yapısal olmayan protein 1 (nsp1) önleyici tedavi için potansiyel hedef proteindir. Konak hücre translasyonunu engelleyen nsp1’in yapısının bilinmesi önemlidir. Bu çalışmada 222 Avrupa izolatında görülen nsp1 mutasyonlarının protein yapıda ortaya çıkarabileceği değişimler yapay zekâ tabanlı bir modelleme yazılımı olan trRosetta kullanılarak modellenmiştir. NCBI Virüs veritabanından elde edilen dizi bilgileri MAFFT çoklu dizi hizalama programı ile hizalanmıştır. Mutasyon analizleri RDP4 yazılımı ile yapılmıştır. Mutant protein primer yapı MegaX yazılımı ile oluşturulmuştur. Protein kalite skorları QMEAN algoritması kullanılarak analiz edilmiştir. Proteinleri fizikokimyasla özellikleri ProtParam ExPAsy programı ile yapılmıştır. Elde edilen protein yapıların konformasyonel analizleri PyMOL ile yapılmıştır. SARS CoV-2 Avrupa izolatlarında görülen nsp1 mutasyonlarının protein sekonder ve tersiyer yapısında konformasyonel ve topolojik değişimlere neden olabileceği tespit edilmiştir. SARS CoV-2 katalitik bölgeyi içine alan P153 ve N178 rezidüleri arasında kalan bölgede görülen değişimin proteinin fonksiyonel özelliklerini etkileyebileceği düşünülmektedir. Elde edilen verilerin önleyici ve tedavi edici yaklaşımlara önemli veriler sunabileceği düşünülmektedir.

    • SDG 3 · Sağlık ve Kaliteli Yaşam
    AtıfOpenAlex1TR Dizin2
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  • MakaleYalnız künye2018
    The Effect of Honey and Pollen on Lipid Peroxidation and Some Antioxidants Against Experimental Liver Damage With Cadmium

    Simsek, Halil; Güler, Osman

    The aim of this study was to determine the changes in cadmium (Cd) expose rats in liver tissue by giving honey and pollen to glutathione peroxidase (GSH-Px), catalase (CAT), glutathione (GSH) and malondialdehyde (MDA). The study was performed on rats and five groups were formed. The groups were designed as following; Group 1: Control, Group 2: Cd administered, Group 3: Cd+honey, Group 4: Cd+pollen, Group 5: Cd+honey+pollen. The application was lasted for six weeks. At the and of the study, tissue samples were collected and the levels of GSH-Px, CAT, GSH and MDA were measured. After statistical analyses, while the differences of amount of tissue GSH-Px, CAT and MDA levels between control and Cd groups were statistically important, the GSH levels were not. Activities of GSH-Px and CAT were important in Cd+honey, Cd+pollen and Cd+honey+pollen groups along with Cd, group. While the level of MDA were important in Cd and Cd+honey, Cd and Cd+pollen, Cd and Cd+honey+pollen, level of GSH in was found unimportant in all groups.

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  • MakaleYalnız künye2014
    Sığırların normal doğum ve güç doğumunda eritrosit arginaz aktivitesi ve plazma nitrik oksit düzeylerinin karşılaştırılması

    Kandemir, Fatih Mehmet; Yüksel, Murat; Benzer, Fulya; Erişir, Mine; Hanedan, Başak

    This study was aimed to investigate erythrocyte arginase activity and plasma nitric oxide (NO)levels in dystocia and normal calving of cows and compare these parameters. The study materialconsisted of 16 cows, 3 8 years old, brought for parturition at term to the Clinic of Obstetrics andGynecology at F ı rat University. Blood samples of cows normally calving and dystocia were takeninto blood collecting tubes with heparin from the jugular vein in the first half hour after delivery andcentrifuged at 3000 rpm for 10 minutes. Plasma NO levels and erythrocyte arginase activities weremeasured. The erythrocyte arginase activity in cows normally calving was significantly increasedcompared to that in cows with dystocia (P&lt;0.01), and the plasma NO level in cows normally calvingwas significantly decreased compared to that in cows with dystocia (P&lt;0.05). It was concluded thatthe lower erythrocyte arginase activity and higher plasma NO level in cows might play a role indystocia.

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  • MakaleYalnız künye2013Scopus SJRQ3
    Antioxidant activity, total phenolic and flavonoid content of water and ethanol extracts from achillea millefolium l

    Türkoğlu, Semra; Keser, Serhat; Çelık, Sait; Türkoğlu, İsmail; Ökkeş; Yılmaz, Okkes; Yilmaz

    Achillea millefolium (Asteraceae, yarrow) has been used in folk medicine against several disturbances including skin inflammations, spasmodic and gastrointestinal disorders. In this study, ethanol and water extracts were prepared from A. millefolium flowers, leaves and seeds. Antioxidant activities were measured by ferric thiocyanate method, and H2O2radical scavenging activity assays and phenolic compounds and flavonoid contents of A. millefolium extracts were also determined. In conclusion, extracts of A. millefolium flowers, leaves and seeds had effective H2O2radical scavenging activity, total antioxidant activity, and these antioxidant activities were compared with BHA and ?tocopherol as reference antioxidants

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