Sürdürülebilir Kalkınma Amaçları

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  • MakaleAçık Erişim2026
    Phylogenetic Analysis of Native Salmo Species in Türkiye Using Mitochondrial 12S rDNA, 16S rDNA, Cytochrome b Sequences, and Cytogenetics

    Yazdıç, Ferit Can; Kar, Bülent; Karaman, Altuğ

    In this study, phylogenetic relationships and genetic diversity of Salmo spp. populations in different water bodies in Türkiye were investigated, focusing on morphologically similar specimens to Salmo macrostigma. Significant genetic variability and evolutionary differentiation within these populations have been identified using mitochondrial regions sequences (12S rDNA, 16S rDNA, and Cyt-b), morphological observations, and cytogenetic analysis. Samples collected from eight river basins revealed three main phylogenetic clades and local adaptations influenced by ecological pressures. Cytogenetic findings showed that they have chromosome numbers ranging from 78 to 80 (2n), consistent with known Salmo species. For this purpose, three mitochondrial DNA fragments encoding 12S rDNA, 16S rDNA, and Cyt-b regions were amplified by PCR, sequenced, and then used for phylogenetic analysis of the studied species. The study revealed the need for comprehensive integrative taxonomic approaches to clarify the classification of Salmo spp. with critical implications for conservation strategies in Türkiye biodiversity hotspots. In conclusion, this study not only advances our knowledge of Salmo macrostigma or similar Salmo spp., but also suggests innovative approaches for future Salmonid phylogenetics and cytogenetics studies. The findings have important implications for understanding the evolution, taxonomy, and conservation of these economically and ecologically important fish.

  • MakaleAçık Erişim2022
    Enzymatic and Bibliometric Analysis of Bifidobacterium sp. of Probiotic Origin in The Based Digestive Tract of Honey Bees

    Yazdıç, Fadime; Kar, Bülent; Karaman, Altuğ; Yücel, Halit; Ekinci, Kübra

    Honey bees (Apis mellifera) are a unique super organism that produces products such as honey, pollen, royal jelly, propolis, beeswax and bee venom and serves as a vector for pollination of many natural and industriyal plants. The effect of intestinal microflora and, accordingly, the immune system come to the fore among the causes of colony losses that have occurred recently. The diversity and densities of bacteria in the digestive tube of bees are affected by their immune systems. In order for this effect to be positive, the intestinal microflora must be natural and balanced. The enzyme activity of the probiotic-derived Bifidobacterium sp., which has an important place among the bacterial populations in the intestine, has a critical effect on both other symbiont microorganisms and the nutrition of the host. Therefore, in this study, both glikozide hidrolase enzyme activity analysis and bibliometric analysis were performed to emphasize the importance of Bifidobacterium sp. Different pH, temperature and substrates were preferred to calculate the optimum enzyme activity. As a result of the analysis of the enzyme activity, the glycoside hydrolase activity (pH 5.0 and 30oC) of Bifidobacterium sp. was determined. Further use of inulin substrate has shown that it is a natural prebiotic source for this probiotic microorganism. At the same time, the hydrolysis of xylan, which is known as a new generation prebiotic source, has also taken place. For bibliometric analysis, correlation links in Web of Science and Scopus database were concluded with the help of VOS viewer software

    • SDG 2 · Açlığa Son
    AtıfOpenAlex2TR Dizin1
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  • MakaleAçık Erişim2022
    Tunceli Uzunçayır Baraj Gölü’ndeki Kababurun (Chondrostoma regium, (Heckel, 1843)) Balıklarının Et Veriminin Belirlenmesi

    Yüksel, Fahrettın; Demirol, Ferhat; Kurt Kaya, Gülderen; Gündüz, Fatih

    Bu çalışma, Uzunçayır Baraj Gölü’nden avlanan kababurun balığı (Chondrostoma regium, (Heckel, 1843))’nın et verimliliğini belirlemek amacıyla yürütülmüştür. Bu amaçla çalışmada, C. regium türüne ait yaşları I-VIII arasında dağılım gösteren 100 adet balık kullanılmış olup, et verimliliği ile farklı vücut kısımları arasındaki ilişkiler incelenmiştir. İncelenen örneklerde, ortalama yüzgeç ağırlığı, omurga ağırlığı, deri ağırlığı, baş ağırlığı, iç organ ağırlığı ve tüketilebilir kısımların vücut ağırlığına göre yüzde oranları sırasıyla %1,72, %3,57, %7,50, %10,55, %9,94 ve %66,72 olarak belirlenmiştir. Çalışmanın sonunda kababurun örneklerinde en yüksek tüketilebilir et oranının III. yaş grubunda (%67,91), en düşük et oranının ise I. yaş (%62,04) grubunda olduğu belirlenmiştir.

    • SDG 2 · Açlığa Son
    • SDG 14 · Sudaki Yaşam
  • MakaleYalnız künye2022
    Effects of Maca Root Extract on Some Molecular Parameters of Kidney Tissue in Rats Fed a High-Fat Diet

    Erten, Fusun

    Maca (Lepidium meyenii) has been used as a dietary supplement in ethnomedicine for centuries.\rMaca has recently become a high-functional food globally, due to its numerous biological benefits.\rThe high-fat diet (HFD) is closely related to the occurrence of obesity. Obesity is a common cause\rfor chronic kidney disease. The aim of this study was to investigate the effects of maca on organic\ranion transporters-3 (OAT-3), organic cation transporter-1 (OCT-1), organic cation transporter-2\r(OCT-2), insulin receptor substrate-1(IRS-1), vascular adhesion molecule-1 (VCAM-1) and\rvascular endothelial growth factor (VEGF) protein levels. In the study, 28 Sprague-Dawley male\rrats were randomly allocated into four groups (n=7); i) Control group (fed with standard diet); ii)\rGroup fed with standard diet and supplemented with Maca (40mg/kg/day) group; iii) Group fed a\rHFD; iv) Group fed with Maca+HFD. After the 60-day of study, kidney OCT-1, OCT-2 and IRS-1\rprotein levels showed a significant increase in the HFD+Maca treated group compared to the HFD\rgroup (P<0.05). Renal OAT-3 level was found to be significantly lower in the HFD group compared\rto the control group (P<0.0001). In addition, a significant decrease in VCAM-1 and VEGF protein\rlevels was observed in the HFD+Maca group compared to the HFD group (P<0.0001). These\rresults show the protective and regulatory activity in the Maca's kidney tissue when both normally\rand the HFD diet is fed

  • MakaleAçık Erişim2022
    Genetic Relationship of Seven Endemic Inula L. (Asteraceae) Species Grown in\rTurkey

    Paksoy, Mehmet Yavuz; Sevindik, Mustafa; Sevindik, Emre

    In this study, genetic relationship of ISSR markers of seven endemic Inula species distributed in\rTurkey was carried out. Plant samples were collected from different regions of Turkey in 2013 and\rgDNA was obtained by DNA isolation from green leaves. Genetic relationship between species was\rdetermined using 12 ISSR primers. PCR products were run on agarose gel electrophoresis and\rvisualized under UV light. All gel images were examined and the presence and absence of\rpolymorphic bands were scored as 0 and 1. A total of 85 bands were obtained from the primers. Of\rthese, 74 polymorphic and 11 monomorphic bands were obtained. The total polymorphism rate was\rfound to be approximately 87.05%. The phylogenetic tree and genetic distances between species\rwere calculated using the PAUP 0 4.0b10 analysis program. According to the distance matrix, the\rgenetic distance was found between the closest Inula helenium subsp. orgyalis and Inula helenium\rsubsp. vanensis (0.29851), while the farthest between Inula sarana and Inula macrocephala\r(0.56000) species. The phylogenetic tree was obtained using the UPGMA algorithm, and the tree\rconsisted of two groups. The results were compared with the morphological, palynological nrDNA\rand cpDNA results of the past. Our findings supported previous studies

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  • Derleme MakaleYalnız künye2021
    Hydrogenosomes in the Anaerobic Fungi: Hydrogen-Producing Organelles

    Yazdıç, Ferit Can; Yazdıç, Fadime; Kar, Bülent; Ozkose, Emin; Ekinci, Mehmet Sait

    Fungi form a very diverse group in eukaryotes. Most of the fungi contain mitochondria and capable of oxidative phosphorylation. On the other hand, anaerobic Neocallimastigomycota fungi are in the gastrointestinal tract of many herbivorous mammals, they contain an organelle known as hydrogenosome instead of mitochondria. These organelles capable of being the key to adaptation to an anaerobic environment that is is unique. In this review, we wish to briefly summarize what is known about function, structure, biochemistry, genetics properties and inheritance of these important organelles.

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  • MakaleYalnız künye2019
    Genetic Diversity Analysis of Aydın/Turkey Dittrichia viscosa (L). Greuter (Asteraceae) Populations Using RAPD Markers

    Paksoy, Mehmet Yavuz; Sevindik, Emre; Uzun, Sefa

    In this study, we performed a genetic diversity using RAPD markers for some Dittrichia viscosa populations grown in the Aydın region of Turkey. Total genomic DNA isolation from the leaves of Dittrichia viscosa was performed using commercial kit. eight RAPD primers were used to determine genetic diversity among populations. Polymerase Chain Reaction (PCR) was performed with all DNA samples and primers with ability of scoreing band. PCR products were run in agarose gel and visualized under UV light. They scored as there were bands (1) and no bands (0) at all gel images and their files were created. A total of 50 characters were obtained from the primers. Phylogenetic relationships and genetic distances between the cultivars were calculated by using the PAUP* 4.0b10 analysis program. According to the PAUP data, the closest genetic distance was determined 0.20000 and between Central and İncirliova populations, the most distant genetic distance were determined 0.36842 between İncirliova and Koçarlı populations. In the phylogenetic analysis has been obtained using UPGMA algorithms and phylogenetic tree consist of two clades. The results also propose that RAPD markers are useful tools for indicating genetic relationships among Dittrichia viscosa populations.

    • SDG 2 · Açlığa Son
  • MakaleYalnız künye2018
    The Effect of Honey and Pollen on Lipid Peroxidation and Some Antioxidants Against Experimental Liver Damage With Cadmium

    Simsek, Halil; Güler, Osman

    The aim of this study was to determine the changes in cadmium (Cd) expose rats in liver tissue by giving honey and pollen to glutathione peroxidase (GSH-Px), catalase (CAT), glutathione (GSH) and malondialdehyde (MDA). The study was performed on rats and five groups were formed. The groups were designed as following; Group 1: Control, Group 2: Cd administered, Group 3: Cd+honey, Group 4: Cd+pollen, Group 5: Cd+honey+pollen. The application was lasted for six weeks. At the and of the study, tissue samples were collected and the levels of GSH-Px, CAT, GSH and MDA were measured. After statistical analyses, while the differences of amount of tissue GSH-Px, CAT and MDA levels between control and Cd groups were statistically important, the GSH levels were not. Activities of GSH-Px and CAT were important in Cd+honey, Cd+pollen and Cd+honey+pollen groups along with Cd, group. While the level of MDA were important in Cd and Cd+honey, Cd and Cd+pollen, Cd and Cd+honey+pollen, level of GSH in was found unimportant in all groups.

    AtıfTR Dizin1
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  • MakaleAçık Erişim2018
    Simmental ırkı sığırlardan elde edilen sütlerde antioksidan aktivitelerin belirlenmesi

    Sahilli, Yeliz Çakır

    Son yıllarda sağlık üzerine olumlu etkileri olan biyoaktif bileşenleri içeren gıdalara olan talep giderek artmaktadır . Süt bu gıda maddeleri arasındadır. Süt, hastalık risklerini azaltıcı ya da önleyici nitelikte antioksidan bileşenlerini de yapısında bulundurmaktadır. Proteinler, vitaminler (E ve C), retinol, beta karoten, glutatyon (GSH), katalaz (CAT), süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GSH-Px) gibi birçok biyoaktif bileşen sütün antioksidan aktivitesini oluşturmaktadır. Bu çalışmada Tunceli ilinde satılan sütlerin GSH düzeyi ile GSH-Px, SOD ve CAT aktivitelerinin araştırılması amaçlanmıştır. Araştırma örnekleri Tunceli ili merkez ilçesinde satılan sütlerden elde edilmiştir. Elde edilen süt parametrelerine ait veriler, süt sığırları için rapor edilen seviyeler ile tutarlı bulunmuştur.

    • SDG 2 · Açlığa Son
    AtıfOpenAlex3
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  • MakaleYalnız künye2013Scopus SJRQ3
    Triploit ve diploit dere alabalıklarında (Salmo trutta fario L, 1758) büyüme performansı, gonat yapısı ve eritrosit boyutunun karşılaştırılması

    Kizak, Volkan; Türel, Murat; Güner, Yusuf; Kayim, Murathan

    The aim of this study was to compare growth, gonadal structure and red blood cell sizes of triploid and diploid brown th trout (Salmo trutta fario). Triploidy was induced by providing a heat shock treatment to the fertilized eggs. From the 18month post-fertilization triploid (TBT) and control diploid (CBT) brown trout groups were investigated up to 32 nd month. Survival, specific growth rate, feed conversion ratio, condition factor, relative growth rate, absolute growth rate, gonadosomatic index, hepatosomatic index and carcass yield were determined in both groups. It has been determined that the triploid fish showed greater live weight than diploid fish despite the difference was not significant (P&gt;0.05). In triploid group triploidisation success was obtained as 95% and male/female ratio was found as 44.4-55.6%. The red blood cells were significantly larger in triploids than diploid ones (P&lt;0.05). Regarding histological section of gonads, triploidy resulted in reduced gonadal development in female, while triploid males seem to exhibit normal gonadal development. Our results suggest that since triploid females could grow more than males, the growth performance studies on triploid brown trout should be performed with all-female stocks under fish farming practices especially for the production of large size trout.

    AtıfTR Dizin3
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  • MakaleYalnız künye2012Scopus SJRQ3
    Keban Baraj Gölünde yaşayan Luciobarbus esocinus heckel, 1843’ün bazı biyolojik özellikleri

    Yüksel, Fahrettın; Türkgülü, İbrahim; Yüce, Songül; Celayir, Yasin; Şen, Dursun; Çoban, Mehmet Zülfü; Yildiz, Nurettin; Eroglu, Mücahıt

    This study was carried out in Luciobarbus esocinus obtained from Keban Reservoir (Elazığ) between March 2008 and October 2009. During the research, it was determined that the percentage of male, female and undefined individuals from 187 L. esocinus were 45.455%, 37.433% and 17.112% respectively. The ages of population were distributed between I and XVII. The lengths of the individuals ranged from 23.0 to 112.4 cm and the weight of them ranged from 118 to 18042 g. The length and weight equation for population was determined as Wall individuals = 0.0057TL3.2187 (n= 187, r= 0.98). The highest GSI values of both sexed were obtained in March and these values were decreased rapidly after this month. It was determined that the condition factor increased until age 5 and then, it decreased respectively.

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