Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Gul, Muhammet(86)
- Güler, Ömer(69)
- Akkurt, Nevzat(68)
- Baskonus, Haci Mehmet(53)
- Tekin, H.O.(52)
- Bulut, Hasan(50)
- ALMisned, Ghada(49)
- Serdar, Osman(43)
- Yucesan, Melih(43)
- Paksoy, Mehmet Yavuz(42)
- Yildirim, Numan(41)
- Zengin, Gokhan(40)
- Yildirim, Nuran Cikcikoglu(39)
- Ince, Muharrem(37)
- Kocabaş, Mehmet(37)
- Celik, Erkan(35)
- Benzer, Fulya(33)
- Sulaiman, Tukur Abdulkadir(29)
- Aslan, Naim(27)
- Can, Erkan(27)
- Kutlu, Banu(27)
- Kök, Mediha(27)
- Tanyol, Mehtap(27)
- Danabas, Durali(26)
- Kutluyer, Filiz(26)
- Özkul, İskender(26)
- Ates, Mehmet(25)
- Yildirim, Ozal(25)
- Onat, Onur(23)
- Topal, Murat(23)
- Demir, Veysel(22)
- Erten, Fusun(22)
- Guler, Omer(22)
- Say, Yakup(22)
- Yaman, Mehmet(22)
- Alataş, Mevlüt(21)
- Güler, Seval Hale(21)
- Çimen, Okay(21)
- Baykal, Duygu Sen(20)
- Mete, Suleyman(20)
Yayınlar
- MakaleAçık Erişim2018Scopus SJRQ4Assessment of student achievement and views on the impact of instruction with visualization of identities in (ax+b)n form in mathematics
Ilhan, Aziz; Çelik, Halil Coşkun
The aim of the present study is to examine the impact of instruction with visualization of identities in form in mathematics on students' achievement and opinions. The study employs an explanatory design, a mixed method, through which qualitative and quantitative data were collected and analysed. Quantitative data in the study were obtained using the Identities and Binomial Exponential Achievement Test to determine the effect of visualization-assisted instruction on students' academic achievement. Qualitative data, regarding the student views on the subject, were collected through a structured interview form. Participants of the study, 53 students, were selected by appropriate sampling method among the first-year univeristy students. The t-test and one-way covariance analysis were used to analyse the quantitative data and the content analysis method was used for the qualitative data. The results obtained from quantitative data indicated that instruction of algebraic expressions through mathematical visualization increased student achievement in comparison to traditional instruction. On the other hand, the qualitative data indicated that visualization facilitated a positive development in instruction of algebraic expressions, rendered the teaching process to be more interesting and enjoyable and led the students to acquire conceptual learning skills instead of memorizing.
AtıfOpenAlex5
İncele - MakaleYalnız künye2018Scopus SJRQ1An interval type-2 fuzzy AHP and TOPSIS methods for decision-making problems in maritime transportation engineering: The case of ship loader
Celik, Erkan; Akyuz, Emre
Duration of cargo operation (loading or discharging) is of paramount importance for ship owners and port managers since idle time at terminal significantly boost to the cost of providing shipping service. Hence, it is important to choose the appropriate shiploader type for handling bulk materials. This paper aims to present a comprehensive multi-criteria decision making (MCDM) method extended by interval type-2 fuzzy sets (IT2FSs) for selecting appropriate shiploader type in maritime transportation. The proposed approach incorporates Analytical Hierarchy Process (AHP) and Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) under IT2FSs environment to overcome more of the uncertainty of experts’ judgement and expression in decision-making. The proposed approach adopts a sensitivity analysis to investigate the impact of key performance indicator (KPI) under numerous conditions. Besides its robust theoretical background, this paper prompts practical benefits to decision-makers (port owners or port managers) who make investment decision for appropriate shiploader type in dry bulk cargo shipment.
AtıfScopus165WoS148OpenAlex172İncele - MakaleYalnız künye2018Scopus SJRQ2A novel wavelet sequences based on deep bidirectional LSTM network model for ECG signal classification
Yildirim, Özal
Long-short term memory networks (LSTMs), which have recently emerged in sequential data analysis, are the most widely used type of recurrent neural networks (RNNs) architecture. Progress on the topic of deep learning includes successful adaptations of deep versions of these architectures. In this study, a new model for deep bidirectional LSTM network-based wavelet sequences called DBLSTM-WS was proposed for classifying electrocardiogram (ECG) signals. For this purpose, a new wavelet-based layer is implemented to generate ECG signal sequences. The ECG signals were decomposed into frequency sub-bands at different scales in this layer. These sub-bands are used as sequences for the input of LSTM networks. New network models that include unidirectional (ULSTM) and bidirectional (BLSTM) structures are designed for performance comparisons. Experimental studies have been performed for five different types of heartbeats obtained from the MIT-BIH arrhythmia database. These five types are Normal Sinus Rhythm (NSR), Ventricular Premature Contraction (VPC), Paced Beat (PB), Left Bundle Branch Block (LBBB), and Right Bundle Branch Block (RBBB). The results show that the DBLSTM-WS model gives a high recognition performance of 99.39%. It has been observed that the wavelet-based layer proposed in the study significantly improves the recognition performance of conventional networks. This proposed network structure is an important approach that can be applied to similar signal processing problems.
AtıfScopus807WoS607OpenAlex837
İncele - Derleme MakaleYalnız künye2018Scopus SJRQ2A review of occupational health and safety risk assessment approaches based on multi-criteria decision-making methods and their fuzzy versions
Gul, Muhammet
Occupational health and safety (OHS) is a multidisciplinary activity working under the tasks of protection of workers and worksites. Risk assessment, as a compulsory process in implementation of OHS, stands out as evaluating the risks arising from the hazards, taking into account the required control measures, and deciding whether or not the risks can be reduced to an acceptable level. The diversity in risk assessment approaches is such that there are many methods for any industry. Multicriteria decision-making (MCDM)-based approaches contribute to risk assessment knowledge with their ability on solving real-world problems with multiple, conflicting, and incommensurate criteria. This article conducts a critical state-of-the-art review of OHS risk assessment studies using MCDM-based approaches. Additionally, it includes fuzzy versions of MCDM approaches applied to OHS risk assessment. A total of 80 papers are classified in eight different application areas. The papers are reviewed by the points of publication trend, published journal, risk parameters/factors, and tools used. This critical review provides an insight for researchers and practitioners on MCDM-based OHS risk assessment approaches in terms of showing current state and potential areas for attempts to be focused in the future.
AtıfScopus181WoS144OpenAlex189İncele - MakaleYalnız künye2018Scopus SJRQ3Curcumin ameliorates doxorubicin-induced cardiotoxicity by abrogation of inflammation, apoptosis, oxidative DNA damage, and protein oxidation in rats
Benzer, Fulya; Kandemir, Fatih Mehmet; Ozkaraca, Mustafa; Kucukler, Sefa; Caglayan, Cuneyt
Doxorubicin (DXR) is a highly effective drug for chemotherapy. However, cardiotoxicity reduces its clinical utility in humans. The present study aimed to assess the ameliorative effect of curcumin against DXR-induced cardiotoxicity in rats. Rats were subjected to oral treatment of curcumin (100 and 200 mg/kg body weight) for 7 days. Cardiotoxicity was induced by single intraperitoneal injection of DXR (40 mg/kg body weight) on the 5th day and the rats sacrificed on 8th day. Curcumin ameliorated DXR-induced lipid peroxidation, glutathione depletion, decrease in antioxidant (superoxide dismutase, catalase, and glutathione peroxidase) enzyme activities, and cardiac toxicity markers (CK-MB, LDH, and cTn-I). Curcumin also attenuated activities of Caspase-3, cyclooxygenase-2, inducible nitric oxide synthase, and levels of nuclear factor kappa-B, tumor necrosis factor-α, and interleukin-1β, and cardiac tissue damages that were induced by DXR. Moreover, curcumin decreased the expression of 8-OHdG and 3,3′-dityrosine. This study demonstrated that curcumin has a multi-cardioprotective effect due to its antioxidant, anti-inflammatory, and antiapoptotic properties.
AtıfScopus154WoS143OpenAlex163İncele - MakaleYalnız künye2018Scopus SJRQ3Investigation of biochemical response in rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) exposed to remazol brillant blue R textile dye
Yildirim, N.C.; Dogan, L.
The dyes used in textile industry production are toxic substances and most of them have polymer structures. These dyes pollute the aquatic environment via various stages such as printing and dyeing, and may cause molecular, cellular, biochemical and physiological disorders. The aim of the present study was to evaluate effect of a highly toxic azo dye, Remazol Brillant Blue (RBBR) on the biochemical responses of rainbow trout, Oncorhynchus mykiss. The fishes were exposed to 0.5, 1 and 2 mg/l of RBBR for 24 and 48 h. The glutathione S-transferase (GST), cytochrome P-450 (CYP1A1) and lactate dehydrogenase (LDH) enzyme activities of these groups were measured. Present dye altered the activities of all these enzymes in the selected tissue of the experimental fish in a dose-dependent manner. According to the results of this study, after dye exposure, the alteration of selected enzymes indicated deterioration in antioxidant defense system, aerobic respiration processes and biotransformation mechanism induced by the tested dye. It was also shown that GST, CYP1A1 and LDH enzymes may be useful as biomarkers in determining the toxic effects in O. mykiss exposed to the RBBR textile dye.
AtıfScopus2İncele - MakaleYayıncıda açık erişim2018Scopus SJRQ3A FUZZY DEMATEL MODEL PROPOSAL FOR THE CAUSE AND EFFECT OF THE FAULT OCCURRING IN THE AUXILIARY SYSTEMS OF THE SHIPS’ MAIN ENGINE
Balin, A.; Demirel, H.; Celik, E.; Alarcin, F.
The ship engine room has a structure that meets a number of needs related to administrative conditions. Even if a simple mechanical error is considered to be the addition of human errors into the complex structure of the engine room, it can lead to undetected loss. How the causes and effects of the detected faults affect the system is as important as an effective fault detection system to detect the fault and take immediate action against any possible engine failure. This study reveals the causes of problems occurring in the main engine auxiliary systems including cooling, lubricating, cooling oil and fuel systems, and the extent of these problems affecting the system. While the Decision Making Trial and Evaluation Laboratory supports to identify and analyze the error detection of auxiliary systems with respect to causal effect relation diagram, fuzzy sets deal with the uncertainty in decision-making and human judgements through the DEMATEL. Therefore, fuzzy DEMATEL approach is applied to examine the causes and the weights of the faults and their relation to each other in the auxiliary systems. When we look at the result of the proposed approach, fuel oil pump failures has more impact on the all system and air cooler problems has the second highest place among the all errors.
AtıfOpenAlex1İncele - MakaleAçık Erişim2018Scopus SJRQ2Observations of crayfish plague infections in commercially important narrow-clawed crayfish populations in Turkey
Kokko, Harri; Harlioglu, Muzaffer M.; Aydin, Hamdi; Makkonen, Jenny; Gökmen, Gökhan; Aksu, Önder; Jussila, Japo
We studied the presence of possible Aphanomyces astaci infections in eight Turkish narrow-clawed crayfish (Astacus leptodactylus) populations by analyzing the prevalence and genotypes of the disease agent A. astaci. The qPCR analyses revealed A. astaci infection in seven of the studied eight populations, with the agent level A2 or higher. The agent levels among the infected populations varied from A0 to A5, i.e., from negative to high level of infection, based on qPCR ranking. Based on the sequencing of the chitinase gene and the mitochondrial ribosomal rnnS and rnnL subunits, we detected both A (As) and B (PsI) haplogroups of A. astaci in our samples, with each of the studied populations being carriers of only one haplotype. The results confirm previous detections of A. astaci in Turkish narrow-clawed crayfish populations and reveal, that both A and B haplogroup A. astaci carriers exist widely in A. leptodactylus populations of Turkey.
AtıfScopus28WoS29OpenAlex23İncele - MakaleAçık Erişim2018Scopus SJRQ1The investigation of the shelf life at 2 ± 1 °C of Luciobarbus esocinus fillets packaged with films prepared with the addition of different essential oils and chitosan
Karaton Kuzgun, Nermin; Gürel İnanlı, Ayşe
In this study, the chemical, microbiological and sensory changes during storage at 2 ± 1 °C of Luciobarbus esocinus fillets coated with edible films prepared with chitosan incorporation of thyme, clove, rosemary essential oils were examined. To create the experimental samples, a total of six groups of L. esocinus fillets coated with different edible films (normal, vacuum-packed, chitosan, chitosan with added thyme oil, chitosan with added clove oil, and chitosan with added rosemary) were used. The food composition of the fillets and experimental samples were determined after they had been coated with edible films. The results of analysis showed that, the preservation period of fresh fillets ended on day 12th, that of vacuum-packed fillets on day 15th, that of fillets coated with chitosan incorporation of rosemary on day 27th, that of fillets coated with chitosan incorporation of thyme and chitosan incorporation of cloves on day 30th. In comparison with the control group, fish spoilage was significantly delayed in samples coated with chitosan incorporation of thyme and chitosan incorporation of cloves (p < 0.05). The lowest bacterial growth, values of PV, TBA and TVB-N were obtained in fish samples coated with thyme + chitosan and cloves + chitosan. In terms of the general acceptability of the fish, as determined by qualified panelists, it was determined that the highest score was given to the experimental group to which essential oil of clove had been applied.
AtıfScopus16WoS12OpenAlex17İncele - MakaleAçık Erişim2018Scopus SJRQ2Investigations of dark, bright, combined dark-bright optical and other soliton solutions in the complex cubic nonlinear Schrödinger equation with δ-potential
Baskonus, Haci Mehmet; Sulaiman, Tukur Abdulkadir; Bulut, Hasan; Aktürk, Tolga
In this study, using the extended sinh-Gordon equation expansion method, we construct the dark, bright, combined dark-bright optical, singular, combined singular solitons and singular periodic waves solutions to the complex cubic nonlinear Schrödinger equation with δ-potential. The conditions for the existence of the obtained solutions are given. To present the physical feature of the acquired result, the 2D and 3D graphs are plotted under the choice of suitable values of the parameters.
AtıfScopus73OpenAlex76İncele - MakaleYalnız künye2018Scopus SJRQ2Effect of the novel biodegradable copolymer and soil salinity on the growth of corn plant
Torğut, Gülben; Beker Akbulut, Gülcin
Biodegradable and biocompatible poly (1,2-epoxy-3-phenoxy propane)-co-(ε-caprolactone) poly(EPP-co-ε-CL) copolymers were synthesized by ring-opening polymerization method at four different ε-CL ratios. Copolymers were characterized using Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance (1H-NMR), thermogravimetric analysis, and differential scanning calorimetry. To examine their possible applications in agriculture, germination test and growth analyses of corn plants were carried out. For this purpose, corn (Zea mays L. cv. “72 May 80”) was allowed to germinate under suitable conditions. Copolymers, 200 mM salt (NaCl) solution, and both copolymers and salt solution were applied to the plants and compared with the control groups. On the 21st day, all the plants were harvested and were stored at −80°C until analysis. Differences in growth parameters, lipid peroxidation degrees, pigment analysis, and total carbohydrate analysis were tested in order to put forward copolymer and NaCl sensitivity of the corn. Statistical analysis was carried out using the SPSS 17.0 software program and the results were compared with each other. These results showed that the copolymers both have a positive effect on the carbohydrate and malondialdehyde (MDA), a product of lipid peroxidation content as well as the chlorophyll content. Moreover, poly(EPP-co-ε-CL0.88) was more efficient than was the other copolymers in the improvement of the detrimental effects of salinity in increasing chlorophyll, carbohydrate, and MDA content, and growth of corn plants. As a result of the study, it was demonstrated that all of the investigated parameters were affected by the applications of salt and copolymers.
AtıfScopus2WoS2OpenAlex3İncele - MakaleAçık Erişim2017Assessment of Crystal Morphology on Uptake, Particle Dissolution, and Toxicity of Nanoscale Titanium Dioxide on Artemia salina
Johnson, M; Ates, M; Arslan, Z; Farah, I; Bogatu, C
AtıfOpenAlex38İncele - MakaleYalnız künye2017Scopus SJRQ3Enhancing the potential of employing thermosetting powder recyclates as filler in LLDPE by structural modifications
Kismet, Yilmaz; Wagner, Manfred H.
Potential of using powder coating recyclates as filler material for polyethylene compounds was investigated. Due to their strong adhesion to the metal surface during compounding at extrusion temperatures higher than 100 degrees C, the coating powder recyclates were chemically modified to facilitate its processing by extrusion and injection molding at higher temperatures. For this purpose, the coating powder recyclates were first hydrolyzed with addition of an alcohol as swelling agent. The deactivated recyclates were mixed at different ratios with the matrix polymer, a linear low-density polyethylene (LLDPE). The mixtures obtained were then compounded by extrusion and subsequently injection molded to plates to produce specimens for testing. The resulting samples were characterized mechanically by tensile, bending, and impact strength tests. In summary, the powder coatings recyclates can be processed as filler for thermoplastic material, without any adhesion problems up to 160 degrees C and 180 degrees C in the extruder and in the injection molding machine, respectively.
AtıfWoS12OpenAlex13İncele - MakaleYalnız künye2017Scopus SJRQ4COMPARATIVE MICROMORPHOLOGY AND ANATOMY OF CHRYSOCHAMELA (FENZL) BOISS. (CRUCIFERAE) GROWING IN TURKEY
Çakilcioglu, Ugur; Paksoy, Mehmet Y.; Babacan, Ebru Yuce; Polat, Ridvan
The micromorphological and anatomical characteristics of three species of Chrysochamela genus have been comparatively presented by using light microscopy (LM) and scanning electron microscopy (SEM). The micromorphological studies are related to the epidermal and seed surface. In anatomical studies, cross sections of the stems and leaves were examined and biometric measurement of cell and tissues were made. The stem and leaf anatomy of the genus were described. In addition, the stomatal index rate and stomatal index of these species were calculated. The leaf is dorsiventral in mesophyll-type. Except its C. noeana taxon, stellate trichomas are coincided in other taxon. Stoma can be observed on both sides of the leaf. Whereas stoma index rates of this genus were found in the range of 0.7 - 1.2, stoma types were observed as aniocytic and anomocytic. Seed surface of the genus can be found in reticulate, verrucae or in verrucaerugose types. In conclusion, the presence/absence of trichome over stem and leaves, the presence/absence of collenchyma in middle the vascular region, the seed shape, epidermal cell shape, and the characteristics of anticlinal boundaries and periclinal cell walls were found to be the important characters for identification of the Chrysochamela genus.
AtıfWoS3İncele - MakaleAçık Erişim2017Co-Author Link Prediction from Academic Databases
Burhan, Yucel; Das, Resul
Social networks: social communication platforms where individuals exhibit their attitudes and behaviors, gestures and mimics in symbolic actions in a virtual environment. Their swift development and wide usage proportion rises social networks' necessity and popularity. People communicate with each other and share some data like idea, thinking, photo, video and location. When this data is processed, very important information is obtained about the users. In this light, it is possible to make important estimates about the users. To analyze a network, the network model, properties of graph structure, criteria and metrics must be calculated first. In this study, similarity calculation between the subjects which authors studied has been done. In this application, five suitable models (Jaccard Index, Sorensen Index, Common Neighbor, L. H. Newman Index and Salton Index methods) are applied to the data set generated by randomly selected twenty writers. In order to evaluate the calculation results; the results of the similarity calculation between authors with known linkages are used as reference values. Comparisons of obtained similarity values with reference values result in new connections. These links reveal that authors can publish articles together.
AtıfWoS1OpenAlex5İncele - MakaleAçık Erişim2017Investigation and monitoring of tetracycline and degradation products in waters of trout farm
Topal, Murat
In this study, tetracycline and degradation products were determined in the basins of a trout farm and in the stream carrying the waters of trout farm. Tetracycline (TC), 4-epitetracycline (ETC), 4-epianhydrotetracycline (EATC) and anhydrotetracycline (ATC) concentrations were determined under the detection limit in upstream of trout farm. The highest and lowest tetracycline concentrations detected in trout farm are 7.64 +/- 0.38 ppb and under detection limit, respectively. The highest and lowest 4-epitetracycline concentrations were 16.2 +/- 0.8 and 1.85 +/- 0.09 ppb, respectively while the highest and lowest 4-epianhydrotetracycline concentrations detected in trout farm were 25.8 +/- 1.3 and 6.12 +/- 0.30 ppb, respectively. The highest and lowest anhydrotetracycline concentrations detected in trout farm were 18.5 +/- 0.9 and 6.24 +/- 0.31 ppb, respectively. Mean tetracycline, 4-epitetracycline, 4-epianhydrotetracycline and anhydrotetracycline concentrations were 3.52 +/- 0.17; 5.30 +/- 0.26; 14.4 +/- 0.7 and 9.64 +/- 0.48 ppb in downstream of trout farm. When upstream and downstream were compared in terms of tetracycline and degradation products it could be said that Keban stream was affected as a result of activity of trout farm.
AtıfWoS1OpenAlex1İncele - Derleme MakaleAçık Erişim2017Usage of microalgae as a sustainable food source in aquaculture
Özçiçek, Esin; Cane, Erkan; Yilmaz, Kadir; Can, Safak Seyhaneyildiz
The need for food sources more reliability according to animal products has increased interest especially in plants and in algae. In aquaculture has benefited significantly from microalgae in the various application areas. They are used as food as well as are evaluated as sources of pigments in the salmonids. Currently, studies are underway to examine the cost-effectiveness of microalgae production systems which can be obtained by large-scale and advanced quality control. In order to ensure sustainability in the use of microalgae; a systems based approach is needed which integrates different fields such as biotechnology, bioprocess and management procedures.
AtıfWoS4OpenAlex1İncele - MakaleYalnız künye2017Scopus SJRQ3SEASONAL CHANGES IN ANTIOXIDANT DEFENCE SYSTEM ON BROWN TROUT (SALMO SP.) IN MUNZUR STREAM
Can, Erkan; Cikcikoglu-Yildirim, Nuran; Erdogan, Devrim
The aim of the present study was to evaluate the oxidant and antioxidant status of the indigenous brown trout (Salmo sp.) caught from Munzur stream (Tunceli, Turkey) at different seasons in different tissues. Glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), total antioxidant status (TAS) and total oxidant status (TOS) levels were measured. These measurements were read in a microplate reader using commercial kits with ELISA method. At the end of the study, significant variations of oxidative stress biomarkers were observed between seasons and tissues. The results of this study show that oxidative stress responses in tissues of Salmo sp. are sensitive to the tissues and the seasons in relation to the contaminants, climatic parameters and reproductive period in Munzur stream. The selected parameters are in valuable biomarkers for monitoring of water systems since at molecular levels they give an early warning signal of xenobiotics on aquatic organisms. It was concluded that the measurement of antioxidants biomarkers known such as SOD, CAT and GSH-Px activities, TAS and TOS levels may also be promising indicators of an oxidant impact on aquatic organisms.
AtıfWoS2İncele - MakaleAçık Erişim2017Stability of fish protein hydrolysate from heads of gilthead sea bream (Sparus aurata), european sea bass (Dicentrarchus labrax) and rainbow trout (Oncorhyncus mykiss) during storage
Erol, Nida Demirtas; Erdem, Omer Alper; Çakli, Sukran
In the present study, protein hydrolyzate was obtained from the head region from the wastes produced during filleting of seabream (Sparus aurata), seabass (Dicentrarchus labrax) and trout (Oncorhyncus mykiss) cultured in Turkey. After the functional and antioxidant properties of the obtained products were determined, the post-storage stability of the protein hydrolysates stored frozen for 6 months was determined by analysis. In this study, degree of hydrolysis, functional properties (protein solubility, emulsion properties, foaming properties, color properties, water holding capacity (WHC)), antioxidant properties (DPPH (1,1 -diphenyl-2-pic-ryhydrayl) free radical scavenging capacity, antioxidative activity, metal chelating) and determination of aminoacid sequencing were carried out in protein hydrolysate. To evaluate the effect of storage time on fish protein hydrolysates stored at -18 degrees C after production, the above-mentioned analyzes including functional and antioxidative properties were repeated at the end of the 6 month storage period. Results were interpreted using statistical analysis. According to the functional and antioxidative properties of fish protein hydrolyzate, the highest antioxidant activity was determined in seabream head hydrolyzate. The production yield of all fish protein hydrolysates is 5%. The functional and antioxidant properties of fish protein hydrolysates varied according to fish species.
AtıfWoS6OpenAlex7İncele - MakaleYalnız künye2017Scopus SJRQ3THE ROLE OF GLUTATHIONE IN THE DETOXIFICATION OF PERCHLOROETHYLENE
Sahilli, Yeliz Cakir
Environmental factors have important roles in the development of cancer. Especially, the exposure of chemicals existing in the workplace with people is a very important issue. People at such places are exposed to chemicals more especially via respiratory and dermal route. Thus, their health is under the risk. Dry cleaning centers are intensively subjected to PCE, which is a common chemical used at these work places, and the risk of cancer is very high for them. The main aim of the present study is to investigate, if PCE exposure results in glutathione efflux from human erythrocytes. In the present study, the changes in intracellular and extracellular non-protein sulfhydryl (NPSH) levels were determined in PCE exposed erythrocytes. PCE significantly depleted intracellular NPSH level in a time and concentration-dependent manner. PCE significantly increased the NPSH levels measured in the extracellular media. Along with the change in extracellular nonprotein thiol levels, significant increases in extracellular glutathione levels were detected. Erythrocyte membranes were not damaged over the course of PCE exposure as indicated by the absence of lactate dehydrogenase activity in the extracellular media. Utilization of MK571 an MRP inhibitor decreased the rate of glutathione efflux from erythrocytes suggesting a role for this membrane transporter in the process. The results of the present study indicate that erythrocytes efflux glutathione when exposed to PCE. In addition to glutathione oxidation, glutathione efflux also contributes to intracellular loss of glutathione when exposed PCE.