Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Yildirim, Nuran Cikcikoglu(5)
- Yildirim, Numan(4)
- Serdar, Osman(3)
- Aksu, Önder(2)
- Kocabaş, Mehmet(2)
- Ogedey, Aysenur(2)
- Tatar, Sule(2)
- Ak, Tuba Parlak(1)
- Aydin, Ayşe Nur(1)
- Benzer, Fulya(1)
- Can, Erkan(1)
- Cikcikoglu Yildirim, Nuran(1)
- Ergin, C.(1)
- Erişir, Mine(1)
- Eşref Gezer(1)
- Gezer, Esref(1)
- Kayim, Murathan(1)
- Korkmaz, Volkan(1)
- Kutluyer, Filiz(1)
- Metin Yaman(1)
- Sahilli, Yeliz Cakir(1)
- Sahilli, Yeliz Çakir(1)
- Samasas, Omer(1)
- Seker, Engin(1)
- Tatar, Şule(1)
- Türkmenoğlu, Yener(1)
- Türkoǧlu, Semra(1)
- Yildirim, N. C.(1)
- Yurekli, Muhittin(1)
- Yürekli, Muhittin(1)
- Çakir Sahilli, Yeliz(1)
Yayınlar
- MakaleAçık Erişim2026Scopus SJRQ3Determination of Oxidative Stress Responses Induced by the Combination of 4 Different Rare Earth Elements in Dreissena polymorpha; [Determinacija odgovorov na oksidativni stres, ki ga povzroča kombinacija 4 različnih redkih zemljinskih elementov v Dreissena polymorpha]
Serdar, Osman; Aydin, Ayşe Nur
Rare Earth Elements (REE), whose usage areas are increasing day by day, are increasing in the amount of mixing with the environment, causing changes in antioxidant enzyme activities by causing oxidative stress in living organisms. In this study, it was aimed to examine the oxidative stress responses induced by the mixture of 4 different REEs (terbium, gadolinium, Lanthanum, Praseodymium) in Dreissena polymorpha. For this purpose, sublethal concentration values were determined by literature review. Experimental application was carried out within 24 and 96 hours. In the analyzes performed to determine biomarker responses, samples taken from living organisms were weighed and homogenization processes were performed for the analysis of samples taken from the experimental groups, including the control group. After homogenization, samples were centrifuged at 4.000 rpm for 15 minutes. Supernatants were kept at -86 °C until measurements were made. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities, and glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) levels were determined using ELISA kits. Statistical analyses were performed using SPSS. One-way ANOVA (Duncan’s multiple range test; p < 0.05) was used for comparison of measured parameters among groups. As a result of the application, a decrease in CAT activity and GSH level and an increase in TBARS levels were observed after 96 hours compared to the control group, while no statistically significant difference was detected in SOD and GPx activities.
- MakaleYalnız künye2025Scopus SJRQ3Oxidative and Antioxidant Responses in Daphnia magna Following Exposure to Sub-Lethal Glyphosate Concentrations
Sahilli, Yeliz Çakir; Korkmaz, Volkan
Abstract: Glyphosate (GLY) is one of the most widely used herbicides in modern intensive agriculture to enhance crop production. However, the chemical additives in commercial formulations and the active ingredient itself can adversely affect biological and chemical processes in aquatic organisms. Therefore, the aim of this study was to evaluate the effects of sub-lethal concentrations of GLY (0, 0.23, 0.58, and 1.17 μg/L) on oxidative stress biomarkers—namely lipid peroxidation (measured as malondialdehyde, MDA), and antioxidant enzymes (superoxide dismutase, SOD; catalase, CAT; and reduced glutathione, GSH)—in Daphnia magna after 24 and 96 h of exposure. The results showed that SOD activity increased in all treatment groups compared to the control at both time points while CAT activity decreased in all groups. GSH and TBARS (thiobarbituric acid reactive substances) levels were significantly elevated after 24 and 96 h in all exposed groups. Overall, D. magna was sensitive to sub-lethal concentrations of GLY, and changes in SOD and CAT activities, as well as GSH and TBARS levels, these biomarkers can serve as useful indicators for detecting glyphosate-induced oxidative stress and water pollution.
AtıfOpenAlex1
İncele - MakaleAçık Erişim2024Scopus SJRQ4Oxidative and physiological effects of carvedilol, a beta–blocker, in Daphnia magna; [Efectos oxidativos fisiológicos del carvedilol, un betabloqueante, en Daphnia magna]
Seker, Engin
Carvedilol is a beta–blocker. Beta–blockers affect the heart and circulation. carvedilol is used to treat heart failure and hypertension. Although this substance has effects in humans and some animals, its effect on the physiological and antioxidant status of daphnids is unknown. The aim of this study was to determine the effect of carvedilol doses (0, 0.125, 0.45 and 0.90 mg·L-1) on physiological activities (heart rate, postabdominal paw activity and thoracic limb movements) and oxidative stress. In Daphnia magna; malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), total glutathione (GSH) and glutathione S transferase (GST) markers were examined. The activity of physiological parameters in D. magna exposed to all carvedilol concentrations was found to be lower than in the control group, and the differences were statistically significant (P<0.01). Application of carvedilol to D. magna resulted in lower GSH content in all groups throughout the experimental period. MDA, SOD, CAT and GST activity were improved. These findings indicate that carvedilol causes physiological and biochemical changes in D. magna. Daphnia species have great potential to provide valuable insights into the mechanisms of human medicine. More comprehensive research is needed on this subject.
- MakaleAçık Erişim2022Scopus SJRQ3Effects of kefir supplementation on body antioxidant-systems, haemolymph heat-shock protein 70 and trypsin, in the crayfish Astacus leptodactylus Eschscholtz (Decapoda, Astacidea)
Aksu, Önder; Türkoǧlu, Semra; Can, Erkan; Erişir, Mine; Kocabaş, Mehmet; Kayim, Murathan; Benzer, Fulya
This study was designed to investigate the effects of dietary kefir on trypsin, heat shock protein 70 (HSP70) as well as the oxidant and antioxidant status of the freshwater crayfish, Astacus leptodactylus. The experiment was performed with the four following treatments: Control group, D1, D2 and D3 (kefir supplemented diet 2, 4, 8 g/kg of crayfish body mass, respectively). Crayfish were stocked into 12 fibreglass tanks (three replicates per treatment) and fed for 45 days. Reduced glutathione (GSH), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and catalase (CAT) in hepatopancreas and muscle were analysed to determine the status of the oxidant/antioxidant balance. In addition, HSP70 and trypsin were measured in haemolymph. Our results indicated that the activity of GSH-Px, MDA and GSH levels decreased in hepatopancreas ( p > 0.05) and muscle tissues ( p < 0.05) with increasing kefir concentration, while CAT activity increased ( p < 0.05). Trypsin and HSP70 insignificantly decreased in haemolymph.
AtıfScopus1WoS1OpenAlex1İncele - MakaleYalnız künye2021Scopus SJRQ2Cellular responses of spirlin Alburnoides bipunctatus spermatozoa exposed to Bisphenol A: Biochemical response and sperm quality alterations evaluation
Kocabaş, Mehmet; Kutluyer, Filiz; Çakir Sahilli, Yeliz; Aksu, Önder
Plastics are one of the global environmental problems owing to their highest production. Bisphenol A (BPA) is used in the manufacturing of plastics including polycarbonate, polyvinyl chloride and resins. In particular, aquatic ecosystems are adversely affected by the occurrence of micropollutants. In addition, BPA as a typical endocrine-disrupting chemical (EDC) seriously influences androgenic activity and reproduction of aquatic organisms, particularly fish as non-target organism. In toxicological assays, sperm cells can be used as a potential tool for reliable and practical assessment. For these reasons, sperm cells were exposed to lower concentrations (0 µg/L, 0.5 µg/L, 1.25 µg/L, 2.5 µg/L and 5 µg/L) of BPA and the study focused on BPA-induced oxidative stress. The enzymatic [glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD)] activities and lipid peroxidation (MDA) were analyzed in spermatozoa. The study findings showed that the activities of SOD and GSH-Px were significantly decreased by BPA but lipid peroxidation (MDA) and CAT acticity increased. Notably, the percentage and duration of motile sperm cells decreased. Collectively, spermatozoa clearly showed the sensitivity even at extremely low concentrations. This is first report to assess the impact of BPA on sperm cells of spirlin Alburnoides bipunctatus.
AtıfScopus6WoS6OpenAlex5İncele - MakaleYalnız künye2021Scopus SJRQ1Toxicological effects of di-(2-ethylhexyl) phthalate in Gammarus pulex: a biochemical and histopathological assessment
Yildirim, Nuran Cikcikoglu; Ak, Tuba Parlak; Samasas, Omer
This study aimed to reveal the toxic characteristics of di-(2-ethylhexyl) phthalate (DEHP) by examining the biochemical and histopathological changes in Gammarus pulex, exposed to different doses of DEHP. For this purpose, the lethal concentration 50 (LC50) value of the DEHP was determined by using a static test and found to be 0.079 ± 0.01 ppm. Three subletal doses of DEHP were applied to the G. pulex for 24 and 96 h. Superoxide dismutase (SOD), catalase (CAT), cytochrome P450 1A1 (CYP1A1), and glutathione S-transferase (GST) activities were measured using commercial ELISA kits. The caspase method, which is an immunohistochemical analysis method, was used to determine the apoptosis that occurred in the G. pulex. The results showed that the CYP1A1 activities decreased in the groups exposed to different doses of DEHP compared to the control group (p > 0.05). CAT activity was found to increase in the application groups at the 24 and 96 h compared to the control group. In addition, it was found that SOD and GST activities increased at the 96 h compared to the control group. In light of the microscope examination of the model organism, hemolymphatic lacunae filled with hemolymph and reduction or absence of hemolymphatic ducts were observed especially in the G. pulex gills. Collapse of the gills and hyperplasia were observed after 96 h. As a result, it is suggested that changes in SOD, CAT, and GST activities can potentially be used as sensitive biomarkers for risk assessment in the environment and increased immunoreactivity in G. pulex caused by DEHP depending on increased application doses and application times.
AtıfScopus6WoS3OpenAlex5
İncele - MakaleAçık Erişim2021Scopus SJRQ4DETERMINATION OF THE ANTIOXIDANT PARAMETERS OF COMMONLY AVAILABLE BLACK PINE (PINUS NIGRA J.F. ARNOLD)
Yildirim, Nuran Cikcikoglu; Gezer, Esref; Yildirim, Numan
The antioxidant parameters of black pine (Pinus nigra J.F. Arnold) needles samples collected from different areas in the province of Diyarbakir, Turkey were studied. Black pine needles samples were collected from three different locations (Egil, Kosuyolu, and Diyarbakir industrial area) in the province of Diyarbakir in summer and winter seasons. The activity of enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione S-transferase (GST) and malondialdehyde (MDA) levels of the samples were determined by ELISA method. Results showed that SOD enzyme activities of the samples collected in summer from Kosuyolu and the industrial area decreased compared to the control group, but increased in the samples collected in winter from the same areas. When compared to the control group, it was observed that the MDA levels of the samples collected from Kosuyolu and the industrial area increased.
- MakaleYalnız künye2020Scopus SJRQ2Investigation of antioxidant responses in Gammarus pulex exposed to Bisphenol A
Tatar, Şule; Türkmenoğlu, Yener
One of the most important environmental problems in the world is micro-pollutants. The aim of this study was to investigate the antioxidant responses of Gammarus pulex to Bisphenol A (BPA), an endocrine-disrupting agent. For this purpose, sublethal concentrations of BPA were applied to G. pulex and biochemical responses were studied. Enzymatic antioxidants superoxide dismutase (SOD) and catalase (CAT) activities and nonenzymatic antioxidants glutathione (GSH) and thiobarbituric acid reagents (TBARS) levels in G. pulex were determined in four different groups during 24 and 96 h. Biochemical biomarkers were measured using commercial kits in a microplate reader. When we compared with control, SOD enzyme activity increased in all groups during both administration periods and CAT enzyme activity decreased in all groups. GSH and TBARS levels were increased after 24 and 96 h of application periods in all groups when compared with control. For changes in SOD and CAT activities and GSH, TBARS levels have been determined to be useful as biomarkers against BPA in G. pulex tissues. It has also been proven that G. pulex is an effective bioindicator that shows BPA pollution in water. The different results of biochemical biomarkers can be evaluated as a marker of possible metabolic processes, and the biochemical response of G. pulex can reveal to some extent the environmental consequences of BPA pollution resulting from industrial waters.
AtıfScopus5WoS3OpenAlex7İncele - MakaleYalnız künye2019Scopus SJRQ4THE TOXIC EFFECT OF CADMIUM ON HUMAN ERYTHROCYTES AND NAC'S PROTECTIVE ROLE
Sahilli, Yeliz Cakir
The toxic effects cadmium (Cd), which is a heavy metal, on human erythrocytes and protective effects of N-acetyl-L-cysteine (NAC) on lipid peroxidation and antioxidant enzymes were investigated. The effects of 1, 50, 150 mu M doses of cadmium and 1mM NAC on the level of malondialdehyde (MDA) in human erythrocytes and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) enzyme activities were investigated. When the Cd was applied alone, it was found that MDA level increased in erythrocytes and SOD, CAT and GPx activities decreased. No statistically significant difference was observed when 1mM NAC administered erythrocytes were compared with the control cells. However, the beneficial effect was seen only in administration doses of Cd at the levels of I and 50 mu M and NAC was observed to have a protective effect. As a result, NAC partially reduced the toxic effect caused by Cd on erythrocytes but could not completely prevent it.
- Yüksek Lisans TeziAçık Erişim2018Metil oranj tekstil boyar maddesine maruz kalan Gammarus pulex'de bazı antioksidan ve detoksifikasyon enzim aktivitesindeki değişiklikler
Metin Yaman
Tekstil boyaları bugün dünyada büyük ölçüde kullanılmaktadır ve tekstil endüstrisi ulus ekonomisinde önemli bir rol oynamaktadır. Boya imalatı, baskı, boyama ve tekstil endüstrilerinden gelen boya kirleticileri çevre kirliliğinin ana kaynaklarını oluşturmaktadır. Bu endüstri kuruluşlarının çıkış suları genellikle renklidir ve atıksuyun alıcı su kaynaklarına doğrudan deşarjı, sucul yaşama hasara neden olacaktır. Bu çalışma, Gammarus pulex'teki bazı antioksidan ve detoksifikasyon enzimi aktivitelerinin değişimlerini kullanarak metil oranj (MO) toksisitesini belirlemek için yapılmıştır. LC50 (letal konsantrasyon 50) değerini belirlemek için statik bir test kullanıldı. MO LC değeri 1737,3 ppm olarak tespit edildi ve sonra G. pulex üç subletal dozda (LC değerinin 1/4; 1/8 ve 1/16) oranında MO'ya 24 ve 96 saat boyunca maruz bırakıldı. ELISA kiti kullanılarak Süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GPX), Sitokrom p450 (CYP1A1), glutatyon S-transferaz (GST) aktiviteleri ve glutatyon (GSH), malondialdehit (MDA) seviyeleri belirlendi. Farklı MO dozlarına maruz bırakılan tüm gruplarda CAT ve CYP1A1 aktivitelerinin arttığı bulunmuştur. GST aktivitesi ve GSH, MDA düzeyleri, farklı MO dozlarına maruz bırakılan tüm gruplarda artmıştır. GPX faaliyetleri tüm gruplarda değişmiş fakat gözlenen bu değişiklik istatistiksel olarak anlamlı bulunmamıştır. Farklı konsantrasyonlardaki MO'nun SOD aktivitesini farklı düzeylerde etkilediği görülmüştür. Çalışmamızda, maruz kalma süresinin, GST ve GSH dışındaki biyokimyasal biyolojik belirteçleri önemli ölçüde etkilemediği bulunmuştur. Sonuç olarak, antioksidan ve detoksifikasyon enzimlerindeki değişiklikler ve lipid peroksidasyonu potansiyel olarak çevre içindeki boyaların risk değerlendirmesi için hassas biyolojik belirteç olarak kullanılabilir ve deşarj düzenlemelerinin yapılmasına katkıda bulunabilir.
- Yüksek Lisans TeziAçık Erişim2018Diyarbakır ilinde farklı bölgelerden toplanan bitkilerdeki antioksidan parametreler kullanılarak hava kirliliğinin izlenmesi
Eşref Gezer
Hava kirliliği hem gelişmiş hem de gelişmekte olan şehirlerdeki en önemli çevresel kaygılardan biridir. Bu çalışmada, Diyarbakır ilinde farklı bölgelerden toplanan Pinus nigra bitkilerindeki antioksidan parametreler kullanılarak hava kirliliğinin izlenmesi amaçlanmıştır. Bu amaçla Pinus sp. Ağustos ve Aralık aylarında Diyarbakır'ın merkezinde üç ayrı yerden (Eğil (kontrol), Koşuyolu (şehir merkezi) ve Organize Sanayi Bölgesi) yaprak örnekleri toplandı. Pinus nigra yaprağında enzimatik antioksidanlar süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GPX), glutatyon S-transferaz (GST) ve malondialdehit (MDA) seviyeleri belirlendi. Biyokimyasal biyobelirteçler, ELISA metodu kullanılarak mikroplaka okuyucuda ölçüldü. Ağustos ayında SOD enzim aktivitesini Eğil (kontrol) ile kıyasladığımızda Koşuyolu ve Organize Sanayi Bölgesinde azalmıştır, ancak aralık ayında kontrol ile kıyaslandığında diğer istasyonlarda artmıştır. CAT enzim aktivitesi kontrol ile kıyaslandığında Koşuyolu ve Organize Sanayi Bölgesinde azalmıştır. Ağustos ayında GPX düzeyleri Eğil ile kıyaslandığında Organize Sanayi Bölgesinde artmıştır. GST enzim aktivitesinde her iki mevsimde istatistiksel olarak önemli bir değişiklik görülmemiştir. MDA düzeyleri Eğil ile kıyaslandığında genel anlamda diğer bölgelerde artış göstermiştir. Diyarbakır ilinde hava kirliliğinin izlenmesinde Pinus nigra yapraklarında SOD, CAT, GPX enzimleri ile MDA düzeylerindeki değişikliklerin yararlı birer biyobelirteç olarak kullanılabileceği ancak GST'nin biyobelirteç olarak kullanılmasının uygun olmadığı sonucuna varılmıştır. Ayrıca, Pinus sp. nin kirletilmiş alanlardaki çevresel hava kalitesini yansıtacak etkili bir biyoindikatör olduğu da kanıtlanmıştır. Biyokimyasal biyobelirteçlerin farklı sonuçları, bitkilerdeki hava kirliliğinin olumsuz etkilerinden sorumlu olası metabolik süreçlerin bir göstergesi olarak değerlendirilebilir ve bitkinin biyokimyasal tepkisi, Diyarbakır'da yetersiz kontrol edilen kentsel hava kirliliğinin çevresel sonuçlarını bir dereceye kadar ortaya koyabilmektedir.
- MakaleYalnız künye2018Scopus SJRQ1Antioxidant biomarkers in Gammarus pulex to evaluate the efficiency of electrocoagulation process in landfill leachate treatment
Serdar, Osman; Yildirim, Nuran Cikcikoglu; Tatar, Sule; Yildirim, Numan; Ogedey, Aysenur
The discharge of landfill leachate into the environment without effective treatment poses a serious threat for the aquatic ecosystems. This present study was undertaken to evaluate whether electrocoagulation process is efficient for treatment landfill leachate (LL) or not by using antioxidant biomarkers in Gammarus pulex. Glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) activities and malondialdehyde (MDA) and glutathione (GSH) levels in G. pulex exposed to untreated, treated, and diluted rates 1/10 and 1/20 in both LL during 24 and 96 h were tested. Physiochemical characteristics of leachate (chemical oxygen demand, electrical conductivity, pH, phosphate, turbidity, NH3, Cl−, and color) were determined pre and post treatment. All physiochemical characteristics of LL decreased after treatment process. GSH-Px and CAT activities and GSH and MDA levels were increased in untreated groups when compared to control (p < 0.05). After treatment by electrocoagulation, MDA and GSH levels and CAT activities were returned to control values. In conclusion, the abilities of LL to stimulate oxidative stress in G. pulex have been proven. The results revealed that antioxidant parameters are useful biomarkers for determining the treatment efficiency of the electrocoagulation process.
AtıfScopus19WoS17OpenAlex23İncele - MakaleYalnız künye2018Scopus SJRQ2The using of Gammarus pulex as a biomonitor in ecological risk assessment of secondary effluent from municipal wastewater treatment plant in Tunceli, Turkey
Tatar, Sule; Cikcikoglu Yildirim, Nuran; Serdar, Osman; Yildirim, Numan; Ogedey, Aysenur
The objectives of this research are the assessment of secondary effluents (SEs) quality by evaluating chemical parameters and biomarkers. For this purpose, MDA and GSH levels were determined by spectrofotometer. SOD, CAT, and GPX activities were determined by using ELISA kit in the groups of control and the groups exposed to SE. The level of MDA and the activities of SOD in Gammarus pulex vary depending on the exposure time. GSH levels and GSH-PX activities increased in G. pulex exposed to the effluent compared to the control during 24 h and 96 h. CAT activities were in SE group were found lower than the control in G. pulex during 24 h and 96 h. Current experimental results show that SOD, CAT, GPX activities, and GSH and MDA levels in G. pulex are sensitive and appropriate responses for assess the effects of anthropogenic contaminants on the aquatic ecosystems, particularly effluent complex mixtures.
AtıfScopus19WoS19OpenAlex22İncele - MakaleYalnız künye2013Scopus SJRQ3THE USE OF LIVER ANTIOXIDANT DEFENSE SYSTEM OF CAPOETA UMBLA (HECKEL, 1843) AS BIOMARKER FOR MONITORING IN WATER QUALITY OF UZUNCAYIR DAM LAKE, TUNCELI, TURKEY
Yildirim, N. C.; Ergin, C.
Domestic wastewaters of Tunceli, are directly discharged to Munzur and Pulumur Rivers without any pre-purification process. The purpose of this study is to reveal the size of pollution caused by domestic wastewaters together with natural contamination by the drainage area of the Pulumur and Munzur Rivers and to monitor physiological changes in fish captured from Uzuncayir Dam Lake. In fish samples, superoxide dismutase, catalase, glutathione peroxidase activities and levels of glutathione and malondialdehyde were determined. Our studies showed that the antioxidant system of Capoeta umbla could be used as biomarker of exposure to aquatic pollutants in Uzuncayir Dam Lake.
AtıfWoS3İncele - MakaleYalnız künye2010Scopus SJRQ3THE ROLE OF METHYLATED ADRENOMEDULLIN IN ENZYMATIC ANTIOXIDANT DEFENSE RESPONSE TO COLD STRESS
Yildirim, Nuran Cikcikoglu; Yurekli, Muhittin
The aim of our study was to investigate the effects of methylated adrenomedullin (met-AdM) on some antioxidant enzymes, such as catalase (CAT), glutathione peroxidase (GSH-P-X) and superoxide dismutase (SOD) in rat kidney and heart tissues after cold stress treatment. Male Wistar rats were divided into 4 groups (control, met-AdM, cold stress and cold stress + met-AdM treatments). The rats were kept at room temperature, exposed to a 16/8-hours light/dark cycle. The rats were maintained at ad libitum (free) feeding habit. In met-AdM treated group, animals received intraperitoneal injection of met-AdM (2 mg/g body weight) once a day during a week. For the cold stress exposure, the rats were kept in separate cages at 10 degrees C for a week. Control group rats were kept at laboratory conditions. CAT, GSH-P-X and SOD enzyme activities were investigated in kidney and heart tissues. In kidney tissue compared to control, CAT and GSH-P-X enzyme activity was decreased but SOD was increased in met-AdM-treated group. In met-AdM+cold stress group, only the SOD enzyme activity was significantly increased but CAT and GSH-P-X decreased. SOD and GSH-P-X enzyme activities were increased by cold stress but CAT was decreased in kidney. In heart tissue, CAT and GSH-P-X activities were decreased but SOD was increased in met-AdM-treated group compared to control. All enzyme activities were decreased in met-AdM+cold stress group as well as by cold stress in heart, with regard to control. Cold stress can disrupt the balance in an oxidant/antioxidant system and cause oxidative damage to kidney and heart tissues by altering the enzymatic and non-enzymatic antioxidant status. We can suggest that met-AdM has possible roles in regulation of stress but we need further studies revealing responses of met-AdM in different tissues.
AtıfWoS3İncele - MakaleYalnız künye2010Scopus SJRQ3Investigation of Some Antioxidant Enzymes Activities Depending on Adrenomedullin Treatment and Cold Stress in Rat Liver Tissue
Yildirim, Nuran Cikcikoglu; Yürekli, Muhittin; Yildirim, Numan
Purpose: The aim of this study is to investigate the compensating effects of adrenomedullin on liver tissue after cold stress treatment. Materials and Methods: Male Wistar rats were divided into four groups as control, adrenomedullin, cold stress and cold stress + adrenomedullin treated. Adrenomedullin was given by intraperitoneal injection (2 mg/g body weight) once a day for a week. For cold stress the rats were kept at 10 C for a week. Catalase, glutathione peroxidase and superoxide dismutase activities were measured in liver tissue. Results: Catalase activity was decreased significantly as compared to control in adrenomedullin treated group (p<0.05). There was no significant difference in superoxide dismutase activity (p>0.05). Glutathione peroxidase activity was increased in adrenomedullin treated group but this was statistically insignificant (p>0.05). All enzyme activities were decreased by cold stress (p<0.05). In Adrenomedullin+cold stress group, only the glutathione peroxidase activity was significantly decreased (p<0.05). Conclusion: It is concluded that cold stress induces decrease in catalase, superoxide dismutase and glutathione peroxidase activities in rat liver. Adrenomedullin treatment corrected only glutathione peroxidase activity while decreasing catalase and superoxide dismutase activities further. The discrepancy may be explained by different responses of antioxidant systems to oxidative stress. It can be concluded that oxidative factors may display unexpected effects on the regulation of glutathione peroxidase activities in the adrenonmedullin case.
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