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MakaleAçık Erişim2022Scopus SJRQ2/r/munzur/2991
Chrysin and flunixin meglumine mitigate overloaded copper-induced testicular and spermatological damages via modulation of oxidative stress and apoptosis in rats
Parlak Ak, Tuba; Tatli Seven, Pinar; Gur, Seyfettin; Gul, Burcu; Yaman, Mine; Seven, Ismail
This study aimed to evaluate the possible protective actions of chrysin and flunixine meglumine on testicular and spermatological injuries experimentally stimulated by copper. We separated 36 male Sprague–Dawley rats into six equal groups: control, chrysin, flunixine meglumine, copper, copper +chrysin and copper +flunixine meglumine. Chrysin (50 mg/kg/bw/po), flunixine meglumine (2.2 mg/kg/bw/ip) and copper (500 mg/kg/bw/po) were administered day to day for 21 days. Copper administration caused significant morphological, physiological and biochemical alterations compared to the control group, which are as follows: production of oxidative stress, thanks to rise in testis lipid peroxidation and fall in antioxidant enzyme concentrations, decrease in sperm quality and increase in morphologic sperm abnormalities, suppression of spermatogenesis and prominent alterations in the testis histomorphology and induction of apoptosis in the testis tissues. On the other hand, compared to the copper group, treatment with chrysin or flunixine meglumine significantly attenuated these alterations. In conclusion, chrysin and flunixine meglumine have benefits such as antioxidant, antiapoptotic and anti-inflammatory against copper-induced testicular and spermatological damages in rats via the modulation of oxidative stress and apoptosis. Consequently, chrysin is a natural product which has comparable therapeutic actions to flunixine meglumine on the male reproductive system.
AtıfScopus4OpenAlex4
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MakaleYalnız künye2022Scopus SJRQ2/r/munzur/2966
Comparative Content, Biological and Anticancer Activities of Heracleum humile Extracts Obtained by Ultrasound-Assisted Extraction Method
Ocal, Mustafa; Altunoglu, Yasemin Celik; Angeloni, Simone; Mustafa, Ahmed M.; Caprioli, Giovanni; Zengin, Gokhan; Paksoy, Mehmet Yavuz; Baloglu, Mehmet Cengiz
As the safety and effectiveness of synthetic drugs remain in doubt, researchers are trying to develop natural medicines from medicinal plants. Herein, ethyl acetate, methanol and water extracts from the Heracleum humile plant were obtained by an ultrasonic-assisted extraction process and the aim was to evaluate some biological effects of the extracts due to the limited data on the pharmacological properties of Heracleum humile in the literature. Weak antibacterial activity was observed on tested bacterial species. The minimum inhibitory concentration and the minimum bactericidal concentration values ranged from 250 to 500 μg/mL. In addition, cytotoxic activity was determined using the MTT test. The strongest findings were determined for ethyl acetate extract on the MDA-MB-231 cell lines at the 48th hour (IC50:97.94 μg/mL), followed by the MCF-7 cell lines at the 24th hour (IC50:103.9 μg/mL). All extracts of Heracleum humile contained mainly flavonoids, phenolic acids and their derivatives, i. e., well-known compounds that possess numerous biological activities such as antioxidant, anti-inflammatory, anticancer, antimicrobial etc. The study results could provide important information that Heracleum humile could be a potential candidate as a natural enzyme inhibitor. It can be concluded that these extracts could be useful in the elementary step of improving novel plant-derived multifunctional pharmaceuticals.
AtıfScopus6OpenAlex11
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MakaleAçık Erişim2022Scopus SJRQ2/r/munzur/2956
The Bioremediation Capacity of Sphingomonas melonis for Methomyl-Contaminated Soil Media: RSM Optimization and Biochemical Assessment by Dreissena polymorpha
Önder Erguven, Gokhan; Serdar, Osman; Tanyol, Mehtap; Cikcikoglu Yildirim, Nuran; Yildirim, Numan; Durmus, Barbaros
The bioremediation capacity of S. melonis for methomyl investigated with RSM. Oxidative stress and neurotoxic response determined in D. polymorpha. COD and TOC levels were measured. GSH, MDA levels and CAT, SOD, AChE activities in D. polymorpha exposed to before (group A) and after (group B) bioremediated media during 24 and 96 h were tested by using ELISA kit. AchE activity was decreased 24th h but increased during 96th h in group B compared with group A (p<0.05). MDA levels and CAT activities were decreased during 24 h and 96 h in group B compared with group A (p<0.05). GSH levels were increased during 24 h and 96 h in group B compared with group A (p<0.05). SOD activity did not show a significant change in group B (p<0.05). Methomyl could be effectively remediated by S. melonis and CAT, AChE activities and GSH, TBARS levels of D. polymorpha are useful biomarkers for evaluating the bioremediation capacity.
AtıfScopus5WoS6OpenAlex6
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MakaleAçık Erişim2022Scopus SJRQ2/r/munzur/2880
The role of cross-sectional geometry of high-head gated conduit in oxygen transfer efficiency
Baylar, Ahmet; Ozkan, Fahri; Yildirim, Ceren Beyza; Aydin, Alp Bugra; Tuna, Muhammed Cihat; Ozturk, Mualla
The concentration of dissolved oxygen is an important indicator of water quality because aquatic life lives on the dissolved oxygen in the water. This can be achieved by using hydraulic structures because of substantial air bubble entrainment at these structures. Closed conduit aeration is a particular instance of this. In the present study, experiments were carried out to investigate the role of cross-sectional geometry of high-head gated conduit in oxygen transfer efficiency. In the first part of this study, the role of cross-sectional geometry of high-head gated conduit in air-demand ratio was investigated. In the second part, the role of cross-sectional geometry of high-head gated conduit in oxygen transfer efficiency was investigated. Results pointed that the cross-sectional geometry of the high-head gated conduit had an important effect on the air-demand ratio and the oxygen transfer efficiency. Moreover, a design formula was obtained for the conduit having the highest oxygen transfer efficiency relating the oxygen transfer efficiency to air-demand ratio, Froude number, gate opening, conduit length and gate opening ratio. There was good agreement between the measured and the predicted values. The obtained results will be useful in future modelling processes and aid the practicing engineer in predicting oxygen transfer efficiency of high-head gated conduits.
AtıfScopus10WoS11OpenAlex11
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MakaleAçık Erişim2022Scopus SJRQ2/r/munzur/2865
Synthesis, characterization, and evaluation of antimicrobial performance of N-[3-(dimethylamino)propyl]methacrylamide based copolymer on pathogens
Pıhtılı, Güzin; Erecevit Sönmez, Pınar; Biryan, Fatih; Torğut, Gülben
In this study, poly{N-[3-(dimethylamino) propylmethacrylamide-co-acrylonitrile}; [P(DMAPMA-co-AcN)] copolymer was polymerized by free radical polymerization (FRP) in the presence of azobisisobutyronitrile (AIBN) initiator at 60°C. The structure of the synthesized polymer was characterized by the Fourier transform infrared (FT-IR), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA) methods. The bactericidal and fungicidal effects of the solutions prepared by adding methanol to this copolymer to prevent the growth of pathogenic bacteria, yeast, and fungi were evaluated by disk diffusion and broth microdilution method. Since the resistance of bacteria, yeast and fungi, which adversely affect human health, to antibiotics is an important issue, and there has not been any study on the antimicrobial properties of this copolymer, it has directed us to investigate its antimicrobial properties. According to antimicrobial susceptibility tests: The polymer solution showed a broad spectrum of antimicrobial action with 12.3–14.3 mm/inhibition zone diameter on Pseudomonas aeruginosa, Klepsiella pneumoniae, Enterococcus faecalis bacteria, Candida tropicalis, Epidermophyton floccosum yeast and fungi and showed a narrow spectrum of effect on inhibition of the growth of Candida albicans with 10.3 mm/inhibition zone diameter. No antimicrobial effects were observed on Staphylococcus aureus. The minimum inhibition concentration (MIC) value on the test microorganisms was determined as 125–31.25 μl. The data obtained prove that the polymer is in the same group as the interaction of bactericidal and fungicidal drugs. It is also important as a polymer with antimicrobial properties. This study aimed to produce a new polymer with antimicrobial properties. This polymer can be a new and yielding antimicrobial representative in different areas.
AtıfScopus1WoS1OpenAlex1
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MakaleYayıncıda açık erişim2022Scopus SJRQ2/r/munzur/2850
Does social support affect menopausal symptoms in menopausal women?
Polat, Filiz; Orhan, Ilksen; Şimşek Küçükkelepçe, Didem
Purpose: To determine the effect of perceived social support of menopausal women on their menopausal complaints. Design and Methods: This descriptive and correlational study was conducted with 505 women. The data were obtained using personal information form, menopause rating scale, and multidimensional scale of perceived social support. Frequency, percentage, t test, analysis of variance Skewness and Kurtosis (±1) and correlation were used in the analysis of the data. Findings: In the study, it was found that there was a positive and significant relationship between menopausal symptoms and social support, and menopausal symptoms decreased as social support increased. Practice Implications: Nurses should provide consultancy for women to increase their social support systems to decrease their menopausal complaints.
AtıfScopus27WoS22OpenAlex33
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MakaleYalnız künye2022Scopus SJRQ2/r/munzur/2836
Detailed Chemical Characterization and Biological Propensities of Malabaila lasiocarpa Extracts: An Endemic Plant to Turkey
Zengin, Gokhan; Zheleva-Dimitrova, Dimitrina; Babacan, Ebru Yüce; Polat, Rıdvan; Çakılcıoğlu, Uğur; Sadeer, Nabeelah Bibi; Costa, Emmanoel V.; Mahomoodally, Mohamad Fawzi; Naviglio, Daniele; Gallo, Monica
This study focused on the biological evaluation and chemical characterization of Malabaila lasiocarpa Boiss. (M. lasiocarpa) (Family: Apiaceae). The phytochemical profile, antioxidant, enzyme inhibitory of the methanolic, aqueous, dichloromethane, hexane extracts were investigated. Based on UHPLC-HRMS analyses, a total of 101 peaks were annotated or identified for the first time in M. lasiocarpa extracts. They include hydroxybenzoic, hydroxycinnamic, acylquinic acids and their glycosides, C- and O-glycosyl and O-diglycosyl flavonoids. In addition, 10 simple mono- and disubstituted coumarins together with 10 furanocoumarins were tentatively annotated. The methanolic extract possessing the highest phenolic (24.36±0.60 mg gallic acid equivalent/g extract) and flavonoid (69.15±0.37 mg rutin equivalent/g extract) content also exhibited the strongest radical scavenging potential against 2,2-diphenyl-1 picrylhydrazyl (21.73±0.42 mg Trolox equivalent/g extract, respectively), and highest reducing capacity (57.81±0.97 and 28.00±0.40 mg Trolox equivalent/g extract, for cupric reducing antioxidant capacity and ferric reducing antioxidant power, respectively). The dichloromethane extract substantially depressed the tyrosinase (73.92±5.37 mg kojic acid equivalent/g extract), α-amylase (0.63±0.01 mmol acarbose equivalent/g extract) and α-glucosidase (0.69±0.02 mmol acarbose equivalent/g extract) enzymes. This study has produced critical scientific data on M. lasiocarpa which are potential contenders for the development of novel phyto-pharmaceuticals.
AtıfScopus6OpenAlex5
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MakaleYalnız künye2022Scopus SJRQ1/r/munzur/2809
Mitotic effects of copper oxide nanoparticle on root development and root tip cells of Phaseolus vulgaris L. seeds
Tasar, Neslihan
Copper oxide nanoparticle (CuO NP) is used widely in many fields in nanotechnology. For this reason, both production, use, and release to the environment are increasing with each passing day. With the increased use of products that contain nanoparticles (NP) (<100 nm), plants and organisms that constitute the food chain are at risk. In the present study, Phaseolus vulgaris L., a very common food plant, was exposed to metal-based CuO NPs. The anomalies that were caused by CuO NP in germination and mitosis of P. vulgaris were investigated. In the trials, a total of 4 groups (Control, 50, 150, and 300 ppm) were formed and examined in three replications. The determination of the accumulation and elimination rate because of NPs in P. vulgaris that was used in the study was made through X-ray diffraction (XRD), scanning electron microscope (SEM), mapping image, and EDX characteristic spectrum analysis. Also, the mitotic effects on germination, root development, and root tip cells of seeds that were grown by treatment with control, 50, 150, and 300 ppm concentrations were investigated. The study was conducted in three replications in a laboratory setting. All concentrations of CuO NPs caused significant decreases in the mitotic index in the root tip cells of P. vulgaris when compared to the control. The mitotic index reached the lowest level, especially at the highest concentration. Multiple analyzes in the study showed that CuO NPs cause abnormalities in cell division such as C-metaphase, distorted metaphase, distorted anaphase and telophase, chromosome breakage, asynchronous division, advanced chromosomes, micronucleus, and loss of genetic material. These findings also support that the Cytogenetic Test of P. vulgaris can be used to evaluate the genotoxicity of new nanomaterials that are used in many consumer products. In this respect, NPs that are taken up by the organisms in the food chain may pose a danger to higher consumer organisms when they accumulate in the tissue. A control mechanism must be established for the use and contamination of these particles and wider studies must be conducted regarding their effects. Highlights: The effects of CuO nanoparticle, which has a very wide usage area, on root development and mitosis of Phaseolus vulgaris L. plant were investigated in the study. The abnormalities of mitotic division on interphase, prophase, metaphase, anaphase, and telophase were visualized. Evaluation was made considering scanning electron microscopy (SEM) and X-ray diffraction (XRD) results as well.
AtıfScopus14WoS10OpenAlex16
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MakaleAçık Erişim2022Scopus SJRQ2/r/munzur/2808
Synthesis and Characterization of Azo Dye Complexes as Potential Inhibitors of Acetylcholinesterase, Butyrylcholinesterase, and Glutathione S-Transferase
Buldurun, Kenan; Aras, Abdülmelik; Turan, Nevin; Turkan, Fikret; Adiguzel, Ragip; Bursal, Ercan
In this study, several metal(II) complexes (Fe, Co, Zn, and Ru) of an azo dye ligand (ADL) that, apart from pyrazole ring (PR), include anisole having (−OCH3) group at the m-substitue of the phenyl (L: (E)-4-((3-methoxyphenyl)diazenyl)-3-methyl-1H-pyrazol-5-amine) were prepared, characterized, and the biological activity studies were carried out. The structures of complexes were identified by elemental analysis, FTIR, UV-vis, mass spectra (Fe(II) and Co(II)), 1H-13C NMR spectra, and TGA-DTA. The azo ligand and its metal complexes were evaluated for their biological properties according to the enzyme inhibitory activities. The lowest Ki values of Co(II) complex against all three GST (19.48±2.17 μM), AChE (22.14±3.74 μM), and BChE (26.39±3.19 μM) enzymes were verified as the most effective enzyme inhibition. The biological activity results suggest that the obtained complexes have good potentials to be used in further analyses to explore new pharmaceutical agents.
AtıfScopus20WoS19OpenAlex18
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MakaleAçık Erişim2022Scopus SJRQ1/r/munzur/2797
Novel of (PLA/PCL blend)/Gd2O3 rare earth oxide nanocomposites: Shape memory effect, thermal, magnetic, and mechanical properties
Özen Öner, Ecem; Pekdemir, Mustafa Ersin; Ercan, Ercan; Say, Yakup; Kök, Mediha; Aydoğdu, Yıldırım
Poly ɛ-caprolactone (PCL) and poly lactic acid (PLA) stand out as biocompatible polymers. In this study, PCL and PLA were blended in 40:60 ratio, respectively, and then the Gd2O3 compound, which is an important earth element oxide, was mixed in PLA–PCL shape memory polymer (SMP) blend at different rates and polymer blend nanocomposites were obtained. First of all, the effects of Gd2O3 oxide on the thermal properties of the SMP blend were determined with a differential scanning calorimeter (DSC) and thermogravimetric analyzer (TGA), and it was determined that Gd2O3 did not have a significant effect on the melting temperature of the blend, while it significantly increased its thermal stability. As a result of the XRD (X-ray diffraction) analysis of the composites at room temperature, it was determined that the semicrystalline blend, with the addition of Gd2O3, increased its crystalline property. When the shape memory effect was examined, it was understood that the additive did not eliminate the shape memory effect. When the magnetic property of the gadolinium oxide-doped composite, which is magnetocaloric oxide, was examined, the magnetic property of the composite changed from diamagnetic to paramagnetic with increasing additives. Finally, the mechanical properties of the shape memory blend composite were investigated and no regular changes were observed in the mechanical properties with increasing Gd2O3.
AtıfScopus38OpenAlex42
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MakaleYalnız künye2022Scopus SJRQ2/r/munzur/2796
Structural, biocompatibility, and antibacterial properties of Ge–DLC nanocomposite for biomedical applications
Arslan, Mehmet Enes; Kurt, Mustafa Şükrü; Aslan, Naim; Kadi, Abdurrahim; Öner, Sena; Çobanoğlu, Şeymanur; Yazici, Ayşenur
Integrative production of new nanocomposites has been used to enhance favorable features of biomaterials for unlocking ultimate potential of different molecules. In the present study, advantageous properties of diamond like carbons (DLC) and germanium (Ge) like greater biocompatibility and antibacterial attributes were aimed to combined into a thin film. For this purpose, 400 nm DLC–Ge nanocomposite was coated on the borosilicate glasses via the magnetron sputtering and surface characteristics was analyzed by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and The Raman spectrum. Biocompatibility analysis were performed by 3-(4,5-Dimethylthiazol-2-yl) (MTT) cell viability assay and Hoechst 33258 fluorescent staining genotoxicity assessments on the human fibroblast cell line (HDFa). Finally, antibacterial properties of DLC–Ge nanocomposite coatings were investigated by Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923) bacterial attachment analysis. As a result of magnetron sputtering coating, nearly 400 nm thick DLC–Ge nanocomposite film showed a smooth, a non-porous, and a dense characteristic. Cell viability analysis showed that Ge–DLC coatings permits %95 cell surface growth of fibroblast cells. Also, there were no significant difference in aspect of nuclear abnormalities compared to the (−) control which showed nonmutagenic features of the thin film. Finally, antibacterial attachment analysis put forth that Ge–DLC coatings inhibits bacterial adhesion as %40 and %25 rates for P. aeruginosa and S. aureus bacterial strains, respectively. From these results, DLC–Ge nanocomposites could be proposed as a potential new biomaterial for various biomedical applications.
AtıfScopus20WoS16OpenAlex19
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MakaleYalnız künye2022Scopus SJRQ1/r/munzur/2759
The relationship between nurses' professional values and caring behaviors
Basit, Gülden; Erkuş Küçükkelepçe, Gülhan; Arslan Şeker, Sevda
Introduction: Professional values give meaning and direction to clinical practices by providing a philosophical framework for nursing practices. The internalization of professional values is quite effective in providing professional service and is very important in terms of service quality. Design: This descriptive study has been conducted to determine the relationship between nurses' professional values and caring behaviors. Methods: A total of 176 nurses participated who worked in hospitals in this study. The data were collected online using the Nurse Information Form, Nurses Professional Values Scale-Revised (NPVS-R), and Caring Behaviors Assessment Tool Nursing Version-Short Form (CBAN-SF). Data analysis was performed using SPSS software, the Mann–Whitney U test, the Kruskall–Wallis test, and the Spearman correlation coefficient. Results: Of the nurses participating in the study, 66.5% are between the ages of 21–30, 80.7% are female, and 71% are university graduates. No statistically significant difference was found regarding professional values between the mean scores from the NPVS-R and CBAN-SF in terms of the nurses' age, gender, position, working style, clinic at which they work, or their educational status. The nurses scored high on the NPVS-R and CBAN. A moderate, significant, and positive relationship was found between nurses' overall scores on the NPVS-R and CBAN-SF. Conclusion: As nurses' perceptions of professional values increase, their perception toward assessing care behaviors also increases. In addition, a relationship exists between nurses' education levels and assessment of care behaviors and between their work experience and their perceptions of professional values. Conducting studies on the subject using different designs is recommended.
AtıfScopus17WoS17OpenAlex21
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MakaleYalnız künye2022Scopus SJRQ2/r/munzur/2742
Ohmic heating: A futuristic method for cooking bulgur
Goksu, Ali; Omac, Basri; Sabancı, Serdal
Bulgur is a nutritious, healthy, and partially cooked food product with long shelf life and produced from wheat grains. In the present study, ohmic heating (OH) was used to cook bulgur as an alternative method to conventional cooking in case of bulgur pilaf due to its advantages including shorter heating time, uniform heat distribution in food, and higher energy efficiency. Bulgur was cooked in 0.1% salt solution to evaluate the effect of OH at various voltage gradients (10, 13, 16, and 19 V/cm) and conventional cooking processes on the textural properties of bulgur and energy consumption. The results showed that for the OH process, the total energy consumption for the cooking of bulgur reduced by approximately 80% compared to that for conventional cooking. In addition, the voltage gradients were significantly related to the cooking time, a crucial parameter for the water absorption of bulgur (p < 0.05). Novelty Impact Statement: Pre-cooked bulgur must be cooked in water by using electric or flame equipment before consumption. Nevertheless, in these systems, the cooking process consumes a pretty long time and causes a vast amount of waste energy. Therefore, alternative methods are needed to save time and energy. Thus, the ohmic heating was used as an alternative cooking process for bulgur prior to consumption.
AtıfScopus3WoS3OpenAlex9
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MakaleAçık Erişim2022Scopus SJRQ1/r/munzur/2735
Effectiveness of the training provided to healthcare professionals in Turkey to recognise the symptoms and risks of child abuse and neglect
Cevik Durmaz, Yadigar; Tuncer Coban, Pelin; Eseroglu Soylemez, Tugba; Aktas, Hakki
Healthcare professionals have a significant role to play in the early diagnosis of child abuse and neglect (CAN), which is important in terms of protecting children's health. The study aimed to measure the change in the participants' level of diagnosing the risks and symptoms of CAN before and after the training by providing a training program to healthcare professionals including the signs of CAN, symptoms and procedures to be followed in this case. All healthcare professionals working in a State Hospital and Family Health Centers in Tunceli, Turkey, and who volunteered to participate in the study were included in the study (n = 125). The data were collected before and after the training by using the Personal Information Form and The Diagnosis Scale of the Risks and Symptoms of Child Abuse and Neglect (DSRSCAN). 39 participants (31.2%) reported that they had previously suspected CAN, and 35 (28%) stated that they had encountered CAN before. The mean DSRSCAN score of the healthcare professionals included in the study before the training was 3.38 (lowest =2.37, highest =3.55), while after the training, it went up to 3.60 (lowest =2.76, highest =5.00), and the difference between the pre-training and post-training DSRSCAN scores was determined to be statistically significant (p < 0.001). It was determined that when healthcare professionals were trained on CAN, their awareness could be further strengthened. Hence, training programs for healthcare professionals on diagnosing and reporting CAN should be updated and increased.
AtıfScopus14WoS15OpenAlex18
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MakaleYalnız künye2022Scopus SJRQ2/r/munzur/2693
Compatibilization of polyvinyl chloride - polymethyl methacrylate polymer blends with maleic anhydride-styrene-methyl methacrylate terpolymer
Yilmaz, Ersen
In this study, nonmiscible polyvinyl chloride (PVC) and polymethyl methacrylate (PMMA) polymers were made miscible by using MA-St-MMA terpolymer as a compatibilizer. Maleic anhydride-styrene-methyl methacrylate (MA-St-MMA) terpolymer was synthesized in our laboratory and used. The synthesized terpolymer was determined by FT-IR spectroscopy. Equal mass PVC/PMMA mixture samples were prepared in series and the compatibilizer terpolymer varying between 2% and 10% by mass was added to the samples and the effect of the added terpolymer on the miscibility was determined viscometrically, thermally, mechanically and, visually. With the addition of the compatibilizer, the two components in the mixture behave like a single polymer with a single glass transition temperature (Tg) and the mixture became compatible. As a result of viscometric experiments, it has been determined that with the added MA-St-MMA terpolymer, the Δb value derivated from the Krigbaum-Wall equation which is the miscibility parameter of a mixture shifted into the positive (+) zone indicating that the two polymers became miscible. By visual analysis, it was observed that the added terpolymer reduced the opaque appearance and the phase separations of the mixture. From the tensile test, a significant improvement in impact toughness was observed. When all the data obtained were evaluated, it was determined that the synthesized MA-St-MMA terpolymer was a suitable compatibilizer for the mixture of PVC and PMMA.
AtıfScopus5WoS5OpenAlex8
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MakaleYalnız künye2022Scopus SJRQ2/r/munzur/2650
Integration of electron beam technology into fresh produce wash water line: Effect of inoculum suspension medium and water quality parameters on the radioresistance of Salmonella Typhimurium ATCC 13311
Omac, Basri; Moreira, Rosana G.; Castell-Perez, Elena
Electron beam technology may be integrated into the fresh produce wash water line, as an advanced oxidation process (AOP). However, prior to implementation, effectiveness of the technology must be assessed. Hydroxyl radical scavengers were used to quantify the role of yield of hydroxyl radicals in inactivation of the selected pathogen in aqueous solution. The combined lethal effect of e-beam treatment with aqueous hydrogen peroxide (H2O2) solution was also investigated. The radioresistance of Salmonella Typhimurium ATCC 13311 in deionized (DI) water, that is radiation D10 values, increased (p <.05) from 0.15 ± 0.00 kGy to 0.18 ± 0.00 kGy and to 0.19 ± 0.06 kGy when peptone water (PW) and phosphate buffered saline (PBS) was dissolved into DI water, respectively. Membrane-permeable ethanol provided better protection for this pathogen than nonpermeable PEG. Results suggest that to minimize the protective effects of inoculating suspending media used for the preparation of Salmonella suspension, 1.0 mM phosphate buffer (PB) should be used in microbial studies of irradiated foods. Nitrate and dissolved organic carbon (DOC) reacting with hydrated electrons (eaq−) and hydroxyl radicals (·OH), respectively, induced more sequestering of ·OH. E-beam treatment with 0.60 kGy dose in aqueous solution with H2O2 led to a reduction of 6.44 ± 0.03 log CFU/mL f S. Typhimurium ATCC 13311.
AtıfScopus3WoS3OpenAlex3
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MakaleAçık Erişim2022Scopus SJRQ2/r/munzur/2589
Determination of trace element and fatty acid levels in tissues of mirror and scaly carp (Cyprinus carpio L., 1758) living in Keban Dam Reservoir (Elazig)
Aydın, Sevinç; Akkoyun, H. Turan; Bayramoglu Akkoyun, Mahire; Bengu, Aydın Ş.; Ekin, Suat; Harlioğlu, Ayşe G.
This study investigated the concentrations of some accumulated trace metals (iron, manganese, zinc and copper) and fatty acids in liver and muscle tissues of mirror and scaly carp varieties (Cyprinus carpio L., 1758) living in Keban Dam Reservoir (Elazig), which is the second-largest artificial lake in Turkey that hosts commercially valuable and preferred fish varieties for human consumption. The inductively coupled plasma mass spectrometry (ICP-MS) method was used to analyse the concentrations of trace elements. Fatty acid levels were measured in a gas chromatography mass spectrometry (GC–MS) device, using a flame ionization detector (FID). Considering the two fish varieties and tissues that were studied, the mean metal concentrations (μg g−1) were found in the following ranges: Fe 12.32–49.97, Mn 0.12–0.62, Zn 10.45–86.59 and Cu 2.74–11.86. Although the lowest levels were detected in the muscle tissue for all the metals examined, the levels in the liver tissue were significantly higher. According to the results of the analyses on fatty acid concentrations, the mean total saturated fatty acid levels in the muscle tissue of the scaly carp and mirror carp varieties were found to be 31.07 and 31.43 (%), respectively, whereas these values for the liver tissue were consecutively 22.36 and 22.08 (%). Successively, the mean total levels of unsaturated fatty acids were determined as 68.93 and 68.40 (%) in the muscle tissue and 77.64 and 77.92 in the liver tissue. This study, with the finding that the trace (metallic) element accumulation rates were below the upper limits of the tolerable range, and the examined fish varieties were rich in fatty acids, shows that mirror carp and scaly carp living in Keban Dam Reservoir are highly nutritious for human diets.
AtıfScopus1OpenAlex2
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MakaleAçık Erişim2022Scopus SJRQ2/r/munzur/2584
Investigation of body image perception, self-esteem, and self-confidence in female-to-male transsexuals before and after sex reassignment surgery
Gümüşsoy, Süreyya; Hortu, İsmet; Dönmez, Sevgül; Alp Dal, Nursel; Ergenoğlu, Ahmet M.
Purpose: The study was aimed at investigatting the changes in body image perception, self-esteem, and self-confidence of female-to-male transsexuals (FtM) after sex reassignment surgery (SRS). Design and Methods: The quasi-experimental study was conducted with the female individuals who undergo FtM SRS between May 2018 and May 2019. The study data were collected using the Personal Information Form, Body Image Scale, Coopersmith Self-Esteem Inventory, and Self-Confidence Scale. Findings: It was observed that the patients' postoperative body image perception, self-esteem, and self-confidence improved. As their postoperative body image perception was higher than was their preoperative body image perception, their postoperative self-esteem and self-confidence levels were also higher. In addition, as their self-esteem increased, so did their self-confidence. Practice Implications: It was found that the patients' postoperative body image perception, self-esteem, and self-confidence improved.
AtıfScopus4WoS6OpenAlex5
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MakaleYalnız künye2022Scopus SJRQ2/r/munzur/2576
Silver/selenium/chitosan co-substituted bioceramic coatings of Ni–Ti alloy: Antibacterial efficiency and cell viability
Say, Yakup; Aksakal, Bunyamin; Sinirlioglu, Zeynep A.
The effects of silver (Ag), selenium (Se), and chitosan (Cht) additives on the antibacterial activity and cell viability of NiTi were investigated. Three different hydroxyapatite (HA)-based bioceramic coatings to improve the antibacterial activity and cell viability of NiTi; HA, HA/Ag, and HA/Se–Cht were applied to NiTi substrates by a sol–gel method. The coated samples were characterized by scanning electron microscopy–electron-dispersive spectroscopy and X-ray diffraction and surface hardnesses were measured. The antimicrobial efficiency of the coatings and uncoated surfaces were tested against Escherichia coli-JM 103 and Staphylococcus aureus-ATCC29293. In vitro cell–material interactions using Saos-2 osteoblast cells were characterized by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mitochondrial activity test) assay for cell viability. A homogeneous, crack-free, and porous surface morphology is achieved in coatings. It has been shown that the Se–Cht additives did not have a significant effect on the surface hardness of the HA coating, but the Ag additive increased the hardness. Through in vitro antibacterial activity and cell viability tests, it was shown that Ag additive to bioceramic coatings significantly increased (p <.05) antibacterial properties but caused a decrease in cell viability. However, although Se–Cht additives did not have a significant effect on antibacterial properties (p <.05), it was observed that they increased cell viability.
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MakaleYalnız künye2022Scopus SJRQ2/r/munzur/2545
Naphthol green B Removal using a Novel Hydrogel Composite: Thermodynamics, Isotherms, Kinetics and Optimization by RSM
Pıhtılı, Güzin; Tanyol, Mehtap; Birkan, Kubra
In this study, the efficiency of the Poly(4-vinylpyridine-co-acrylamide) doped with EuCl3 {(Poly(4-VP-co-AAM)/EuCl3)} hydrogel synthesized in the laboratory by free radical in-situ polymerization method in removing naphthol green B (NGB) from wastewater was investigated. The structural properties of the adsorbent have been described by Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and X-ray diffraction (XRD) analyses. The dependence of NGB adsorption performance on varying such as the amount of adsorbent, initial NGB concentration and contact time were investigated in a batch system. To determine the interaction effects of the selected parameters on the adsorption process and to optimize the adsorption capacity of the process, the central composite design (CCD) in the response surface methodology (RSM) was used. Optimum conditions were found to be 110 mg/L NGB concentration, 0.05 g/100 mL Poly(4-VP-co-AAM)/EuCl3/EuCl3 dosage and 165.81 min contact time. The adsorption capacity and removal efficiency under these conditions were 206.46 mg/g and 92.50 %, respectively. Experimental equilibrium data were in better agreement with the Langmuir isotherm. The kinetic results indicated that the adsorption process can be defined by pseudo-second-order kinetic with internal diffusion as one of the rate-determining steps. The adsorption process was found to be exothermic and spontaneous. Experimental results showed that dye can be successfully removed from wastewaters using Poly(4-VP-co-AAM)/EuCl3 hydrogel as adsorbent.
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