Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Kutluyer, Filiz(4)
- Benzer, Fulya(3)
- Can, Erkan(3)
- Erisir, Mine(3)
- Guner, Yusuf(3)
- Ispir, Unal(3)
- Kayim, Murathan(3)
- Kocabaş, Mehmet(3)
- Seker, Engin(3)
- Ögretmen, Fatih(3)
- Inanan, Burak Evren(2)
- Kizak, Volkan(2)
- Kocabaş, Filiz Kutluyer(2)
- Turel, Murat(2)
- Aksu, Önder(1)
- Akça, Ayşe(1)
- Baldemir, Pinar(1)
- Büyükleblebici, Serhat(1)
- Dogan, L.(1)
- Dörücü, Mustafa(1)
- Erecevit Sönmez, Pınar(1)
- Erişir, Mine(1)
- Erol, Nida Demirtas(1)
- Fadli, Nur(1)
- Gulsafak, I(1)
- Gur, Seyfettin(1)
- Hafizuddin, Hafizuddin(1)
- Handayani, Luvi S.(1)
- Karahan, M.(1)
- Karahan, Murat(1)
- Kaya, Emre(1)
- Kaya, Seyma Ozer(1)
- Kizak, V.(1)
- Kocabas, Filiz Kutluyer(1)
- Kocabas, Mehmet(1)
- Koprucu, Kenan(1)
- Kucukgul, A.(1)
- Kuzgun, Nermin Karaton(1)
- Muchlisin, Zainal A.(1)
- Ozcan, Sinan(1)
Yayınlar
- MakaleAçık Erişim2026Scopus SJRQ2Effect of Dietary Supplementation with Omega-3 Fatty Acids on Seminal Plasma Arginase Activity and Semen Quality Parameters in Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792)
Kaya, Seyma Ozer; Ozcan, Sinan; Kaya, Emre; Gur, Seyfettin; Koprucu, Kenan; Sonmez, Mustafa; Turk, Gaffari; Erisir, Mine
Background: This study was conducted to determine the effect of various fatty acid ratios added to diets of rainbow trout (Oncorhynchus mykiss, Walbaum) on some sperm parameters and parameters related to arginase activity. Methods: A total of 99 male rainbow trout were used in this study, with the fish averaging 3 years old. Semen was collected by abdominal massage. Four different experimental groups were created in the study: a control group without n-3 series fatty acids added to their diets, and groups with n-3 series fatty acids added at ratios of 1% (D1), 2% (D2), and 3% (D3), respectively. Results: While no significant difference in semen volume was observed between the D1 and D3 groups and the control group, semen volume was significantly higher in the D2 group. Compared with the control group, the time required for sperm motility to decline to both 50% and 0% was significantly prolonged in the D1 and D3 groups. Seminal plasma arginase activity did not differ significantly in the D2 and D3 groups compared with the control group but was significantly higher in the D1 group. Conclusions: Consequently, considering the doses used in the study, fatty acid supplementation was found to lead to changes in sperm quality parameters, specifically volume (only 2%) and arginase activity (only 1%). Given that the rate of decrease in sperm motility was later in groups D1 and D2, it was concluded that the added fatty acids may be beneficial.
- MakaleYayıncıda açık erişim2026Scopus SJRQ2Sperm cryopreservation of sex-reversed rainbow trout (Oncorhynchus mykiss): incorporation of amino acids and anti-oxidants to the extender media to improve the anti-oxidant system
Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Benzer, Fulya; Erişir, Mine
Anti-oxidants are vital for protecting sperm and can mitigate the negative effects associated with cryopreservation. This study was conducted to evaluate the protective effects of adding anti-oxidants (vitamins and amino acids) to extender in sex-reversed rainbow trout (Oncorhynchus mykiss) sperm. The collected sperm was diluted at the ratio of 1: 5 by the extenders supplemented with different anti-oxidants including 1.00 mM of ascorbic acid and L-tryptophan, 2.00 mM of cysteine and α-tocopherol, and their combination. After dilution, the semen was aspirated into 0.50 mL straws, and the straws were placed on the tray, frozen for 10 min, and plunged into liquid nitrogen. Straws were thawed in a 30.00 ˚C water bath for 15 sec. The Sperm Class Analyzer System was used to evaluate sperm kinematics. The activity of anti-oxidant enzymes and lipid peroxidation were determined as oxidative stress indices. Our data indicated that the incorporation of anti-oxidants and amino acids increased sperm motility duration. The elevated activity of glutathione peroxidase, superoxide dismutase, and catalase in post-thaw samples indicates that the anti-oxidant system in sex-reversed rainbow trout sperm likely plays a crucial role in protecting membrane compounds from oxidation. In conclusion, the combination of 1.00 mM L-tryptophan and ascorbic acid to the extender media caused a prolonging effect in sperm motility after thawing and they have the potential to serve as effective agents for improving sperm cryosurvival.
- MakaleAçık Erişim2026Scopus SJRQ2The Positive Effect of Antioxidants in Maintaining the Post-Cryopreserved Sperm Quality of Rainbow Trout Oncorhynchus mykiss (Walbaum, 1792)
Handayani, Luvi S.; Rahayu, Sri R.; Razi, Nanda M.; Pane, Elya P.; Kocabas, Mehmet; Kocabas, Filiz Kutluyer; Hafizuddin, Hafizuddin; Fadli, Nur; Sulistiono, Sulistiono; Muchlisin, Zainal A.
Rainbow trout aquaculture is challenging due to the restricted spawning seasons. Sperm cryopreservation technology can help ensure a successful spawning season, but its effectiveness is restricted by oxidative stress during freezing and thawing, which reduces the availability of male gametes. This study aimed to evaluate the effect of several types and concentrations of antioxidants on post-cryopreservation rainbow trout sperm quality. Subsequently, a factorial completely randomized design with three replications was applied, testing four types of antioxidants Ascorbic acid, Myo-inositol, Butylated hydroxytoluene (BHT), and Alpha-tocopherol at concentrations of 0, 20, 40, and 60 mM, respectively. The evaluation parameters included motility rate, sperm lifespan, and sperm motion characteristics using the Computer Assisted Sperm Analysis system. The results showed that the type and concentration of antioxidants significantly affected sperm motility and sperm lifespan (P<0.05). The treatment method carried out using 40 mM Ascorbic acid and 20 mM BHT led to the highest sperm motility of 46.67% and sperm lifespan of 34 seconds. Although both antioxidants showed similar effectiveness, BHT at a concentration of 20 mM is more recommended due to its lower toxicity and technical as well as economic efficiency considerations.
- Yüksek Lisans TeziAçık Erişim2023Tunceli ilindeki alabalıklarda Aeromonas türlerinin MALDI-TOF MS yöntemiyle identifikasyonu
Çilem Sevgül Tütmez
Aeromonas cinsi, son 20 yılda bir dizi taksonomik ve terminoloji revizyonlarından geçmiştir ve sürekli olarak yeni (alt) türler ve biyogruplar tanımlanmıştır.Aeromonas cinsi, çoğunlukla fırsatçı mikroorganizmalar olarak işlev gören otuzdan fazla Gram-negatif bakteri türünü içerir.Bu bakteriler, balık ve kabuklular gibi hayvanlardan kolayca izole edildikleri çeşitli su ekosistemlerinde doğal olarak dağılmıştır.Biyokimyasal karakterizasyon gibi standart tanımlama yöntemlerinin eksiklikleri vardır ve taksonomik konumun netleştirilmesine izin vermez.Bu çalışma, klinik ve çevresel Aeromonas izolatlarının hızlı bir şekilde tanımlanması için bir matris destekli lazer desorpsiyon/iyonizasyon-uçuş süresi kütle spektrometresi (MALDI-TOF MS) tanımlama veri tabanının geliştirilmesini açıklamaktadır. Bu çalışmada Nisan, Mayıs, Aralık, Ocak aylarında Tunceli ili Mazgirt, Ovacık, Çemişgezek ve Pertek ilçelerinde ağ kafesler ve beton havuzlarda bulunan 16 tesisten temin edilen 200 adet Gökkuşağı alabalığının karaciğer, bağırsak, dalak ve böbreklerinden alınan doku örnekleri, 64 adet su numunesi analiz edilmiştir. Bu çalışmanın amacı, Tunceli ilindeki tatlı su balık çiftliklerinde Aeromonas türlerinin prevalansını ve prevalansını etkileyen faktörleri belirlemektir.Ayrıca Aeromonas'ın tür sınıf tayini için kullanılan MALDI-TOF MS, 16S rDNA dizileme ve housekeeping gen dizileme (gyrB) gibi farklı yöntemlerin karşılaştırılması amaçlanmıştır.Geleneksel yöntemle izole edilen 526 şüpheli izolatın MALDI TOF-MS ile analizi sonucunda; Basillaceae, Comamonadaceae, Enterobacteriaceae, Enterococcaceae, Erwiniaceae, Moraxellaceae, Nocardiaceae, Pseudomonadaceae, Sthaphylococcaceae, Streptococcaceae, Xanthomonadaceaea, Yersiniaceae ve Aeromonadaceae olmak üzere 13 familyaya ait 526 bakteri tanımlanmıştır.Tunceli ili Mazgirt, Ovacık, Çemişgezek ve Pertek ilçelerinde ağ kafeslerde ve beton göletlerde yetiştirilen dört ilçedeki 16 çiftlikte yapılan kesitsel çalışmada, Aeromonas'ın 5 türü 16S rDNA ve gyrB sekanslama ile teşhis edildi.Balık çiftliklerinde beş Aeromonas türü daha yaygındı, A. sobria %47,52) en yüksekti, bunu A. veronii (%26,95), A. media (%12,06) ve A. hydrophila (%7,80) izledi. Daha az yaygın olan tür ise A. salmonicida (%5,67) idi. Aeromonas türlerinin prevalansında mekansal varyasyon gözlendi. Çalışmamızda, Aeromonas türü olarak identifiye edilen 141 adet izolatın MALDI-TOF MS sonucunun doğruluk saptama oranı, cins düzeyinde tanımlamada %100 (141 izolat) ve tür düzeyinde tanımlamada %92,2 (130 izolat) olduğu tespit edildi.MALDI-TOF MS yönteminin moleküler analizlerde doğru ve hızlı sonuç vermesi nedeniyle kullanılması önerilmektedir. Ancak, Aeromonas cinsinin filogenetik çalışmalarında türler arasında ayrım yapmak için daha yüksek kapasite göstermeleri nedeniyle tür düzeyinde tanımlamada ayrım yapılamadığı noktada 16S rDNA ve gyrB gen dizileme yöntemlerinin daha güvenilir yöntemlerolduğu kanısına varılmıştır. Ülkemizde, Yukarı Fırat havzasında ve Tunceli ilinde gökkuşağı alabalıklarında MALDI-TOF MS yöntemiyle Aeromonas türlerinin tanımlanmasına yönelik yapılan bu çalışma, konusu itibariyle öncü çalışmalardan biri olma niteliğini taşımaktadır.Çalışmamız bu alanda ileride yapılacak araştırmalar için yol gösterici olacaktır.Bu, tatlı su balık çiftliklerinde Aeromonas türlerinin prevalansını belirleyen ve prevalansını etkileyen faktörleri belirleyen ilk çalışmadır.Bu bilgi, balıklardaki Aeromonas enfeksiyonunun ve bunların halk sağlığı üzerindeki risklerinin yönetilmesinde faydalı olacaktır.
- MakaleAçık Erişim2023Determination of lipid quality and mercury levels of sardine and rainbow trout cooked with different methods
Çakli, Sukran; Erol, Nida Demirtas; Sen Yilmaz, Evren Burcu; Baldemir, Pinar; Çakli, Atilla
This study aimed to investigate the effects of baking and pan-frying methods on the lipid quality and mercury (Hg) levels of two important fish species in Turkiye, namely, fileted sardine (Sardina pilchardus) and rainbow trout (Oncorhynchus mykiss). The results revealed that sardines significantly decreased n-3 fatty acids depending on the cooking process, while the best n-6/n-3 ratio was observed in baked sardines, with higher rates found in pan-fried fish. Notably, pan-fried rainbow trout cooked with butter showed the highest atherogenic index (AI) of 0.71 +/- 0.32 and thrombogenic index (TI) of 0.61 +/- 1.43, as well as a hypocholesterolemic/hypercholesterolemic index (HH) of 0.79 +/- 0.17. Conversely, fried sardines exhibited lower atherogenic and thrombogenic scores, with fried sardines cooked in sunflower oil having a hypocholesterolemic/hypercholesterolemic index of 4.85 +/- 0.3. There were no significant variations in Hg content between raw and cooked fish. However, when compared to the raw control, the rise in Hg content for baked fish was substantial (p < 0.05) (baked rainbow trout 0.18 mg/kg and sardine 0.29 mg/kg). The decrease in FAs (Fatty Acids) due to cooking methods can be ordered as follows: Raw sardine > baked > fried. Conversely, the increase in FAs due to the cooking methods can be ordered as follows: fried > baked > raw sardine. Baked rainbow trout had much lower levels of palmitic acid, stearic acid, and myristic acid than the raw material. However, these levels were significantly increased in rainbow trout cooked in a frying pan. Additionally, oleic acid levels in fried rainbow trout were detected to be lower than in other preparations, whereas they were equivalent in baked rainbow trout.
AtıfWoS1OpenAlex2TR Dizin1
İncele - MakaleYalnız künye2022Scopus SJRQ3Ascorbic acid ameliorated the sperm quality of rainbow trout (Oncorhynchus mykiss) against arsenic toxicity: Impact on oxidative stress, fertility ability and embryo development
Kocabaş, Filiz Kutluyer; Kocabaş, Mehmet; Aksu, Önder; Çakir Sahilli, Yeliz
Arsenic (As) is a heavy metal and aquatic pollutant and adversely impacts the reproduction of male fish. As a chain-breaking antioxidant, ascorbic acid (AA) has high water solubility and low toxicity. In this context, the current study was performed to assess the protective role of AA (1 mM) on the sperm cells of the rainbow trout (Oncorhynchus mykiss) exposed to sublethal concentrations of As (8, 16 and 32 mg/L). Sperm quality parameters were analyzed using a sperm class analyzer system. Lipid peroxidation and antioxidant enzyme levels were used as indicators of oxidative stress. The fertilization, eyeing and hatching rates were determined as gamete markers. Reduced sperm quality parameters and fertility capacity resulted from in vitro exposure to As (P < 0.05). The oxidative stress in sperm cells increased after As exposure (P < 0.05). The presence of AA improved sperm movement parameters and fertility potential (P < 0.05). Overall, AA had a positive effect on oxidative stress and fertility ability against As toxicity and AA supplementation ameliorated detrimental effects of As in sperm cells.
AtıfScopus11WoS9OpenAlex5
İncele - MakaleAçık Erişim2022Scopus SJRQ2Comparison of nutrient contents of wild and farmed rainbow trout (Oncorhynchus mykiss, Walbaum 1792) from Keban Dam Lake in Eastern Anatolia region of Turkey
Özçiçek, Esin; Can, Erkan; Yılmaz, Ökkeş
In this study, fatty acids, fat-soluble vitamins (A, D, E, K vitamins) and cholesterol contents of wild-caught and farm-raised rainbow trout (Oncorhynchus mykiss) were compared. The amounts of docosahexaenoic acid (22:6 n-3, DHA) in wild and farmed forms were 21.56%–18.14%, 18.79%–16.21% and 14.34%–11.49% in April, May and June, respectively. Vitamin E, which is one of the fat-soluble and antioxidant vitamins, showed highest level among the vitamins. Vitamin E levels in both wild and farmed fish were 5.11–4.26, 4.83–2.89 and 0.99–1.95 mg/100 g in April, May and June, respectively. The substantial difference between wild and farmed rainbow trout was found in May (p < 0.05). In conclusion, both wild and farmed rainbow trout from Keban Dam Lake (Pertek Region) can be considered as good sources of n-3 polyunsaturated fatty acids (PUFA). However, it was observed that the n-3 PUFA level in wild form was higher than the culture fish within the investigated monthly periods. On the other hand, farmed fish demonstrated higher levels of n-6 PUFA over the wild individuals.
AtıfScopus11WoS9OpenAlex8İncele - MakaleYalnız künye2021Scopus SJRQ3Glyphosate disrupts sperm quality and induced DNA damage of rainbow trout (Oncorhynchus mykiss) sperm
Akça, Ayşe; Kocabaş, Mehmet; Kutluyer, Filiz
As a widespread pollutant, glyphosate (GLY) adversely affects the aquatic environment and can impair the reproductive ability and functions of fish. The purpose of the current study was to assess in vitro effect of GLY on rainbow trout (Oncorhynchus mykiss) sperm cells. The sperm cells were exposed to different GLY concentrations (2.5, 5, 10 mg/L). Sperm motility parameters were analyzed with computer assisted sperm analysis. DNA fragmentation (%) was measured by the comet assay using fluorescence microscopy. With increased GLY concentration, sperm motility and duration decreased after exposure. DNA fragmentation (% DNA in tail) in sperm cells was higher in treatments containing GLY than control (p < 0.05). Consequently, sperm cells are sensitive to low doses of GLY, and this can negatively affect natural populations.
AtıfScopus8WoS4OpenAlex5İncele - MakaleYalnız künye2020Scopus SJRQ4Preservative effect of coriander essential oil applied in different proportions on the storage of rainbow trout (Oncorhynchus mykiss) fillets
Kuzgun, Nermin Karaton; Erecevit Sönmez, Pınar; Tanyildizi, Muhammet Şaban
Aim of this study, the sensorial, microbiological, and chemical features during shelf life at 2 ± 2 °C of rainbow trout fillets samples treatment with coriander essential oils were examined of protective effect of oils. According to the findings of the research, the storage during of fillets ended on day 9th, that of control on day 12th, that of samples applied with coriander oils 0.1% on day 15th, which of fillets applied with coriander oils 0.25% and applied with coriander oils 0.5% on day 18th. The minimum number of bacteria, values of TVB-N and TBA were founded in trout fillets treatment with 0.5% coriander essential oil. In terms of the sensory quality of the fillets, as assessed by panelists participating in the panel, it was detected that highest point was given to the fillets groups to which coriander essential oil of 0.5% had been enforced, and as a result, with this application, it was underlined that was obtained in a positive direction of a flavor very different other than ordinary fish flavor by panelists.
AtıfScopus5WoS2İncele - MakaleYalnız künye2019Scopus SJRQ3Bio-functions of carvacrol-supplemented feeds on lipopolysaccharide-induced rainbow trout (Oncorhynchus mykiss Walbaum, 1792)
Kucukgul, A.; Gulsafak, I
To investigate the effects of carvacrol supplementation in rainbow trout induced by lipopolysaccharide (LPS), relative gene expressions levels of some pro-inflammatory cytokines and apoptosis markers in kidney and liver were measured. Fish with a mean weight of 44.71 +/- 1.33 g were studied using four different treatments with three replicates each. Inflammation and apoptosis were performed using LPS of Escherichia coli (25 mu g ml(-1)) except in the control group and only carvacrol (100 mu g ml(-1) diet) containing diets were fed to this group (CAR). The last group was the infected fish fed carvacrol supplemented diet (+CAR). Kidney and liver tissues were removed 3 days after to determine the levels of interleukin-1 beta (IL-1 beta), interferon gamma (INF-gamma), caspase 3 (Cas 3), caspase 8 (Cas 8) using Real-Time PCR analyses. IL-1 beta expressions of both kidney and liver was significantly decreased (12.9 and 2.14 fold, respectively) in LPS treated cells (p<0.05). While IFN-gamma expression was up regulated in kidney, it had down regulation in liver. LPS decreased both Cas 3 and 8 expressions in kidney but increased in liver. +CAR increased expressions of IL-1 beta and IFN-gamma compared with the control (up to 2-3 fold) in all tissues except caspase gene expressions that were similar in the control. In all tissues, IL-1 beta and IFN-gamma expressions increased in +CAR group, expect IFN-gamma in the kidney. However, Cas 3 and 8 expressions including apoptosis was induced by up-down regulations in all tissues compared to LPS-injected fish. The results showed that carvacrol had pro-inflammatory and apoptotic effects especially on liver tissue of LPS-induced inflammation model of Oncorhynchus mykiss.
AtıfWoS4OpenAlex3İncele - MakaleYalnız künye2018Scopus SJRQ3Investigation of biochemical response in rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) exposed to remazol brillant blue R textile dye
Yildirim, N.C.; Dogan, L.
The dyes used in textile industry production are toxic substances and most of them have polymer structures. These dyes pollute the aquatic environment via various stages such as printing and dyeing, and may cause molecular, cellular, biochemical and physiological disorders. The aim of the present study was to evaluate effect of a highly toxic azo dye, Remazol Brillant Blue (RBBR) on the biochemical responses of rainbow trout, Oncorhynchus mykiss. The fishes were exposed to 0.5, 1 and 2 mg/l of RBBR for 24 and 48 h. The glutathione S-transferase (GST), cytochrome P-450 (CYP1A1) and lactate dehydrogenase (LDH) enzyme activities of these groups were measured. Present dye altered the activities of all these enzymes in the selected tissue of the experimental fish in a dose-dependent manner. According to the results of this study, after dye exposure, the alteration of selected enzymes indicated deterioration in antioxidant defense system, aerobic respiration processes and biotransformation mechanism induced by the tested dye. It was also shown that GST, CYP1A1 and LDH enzymes may be useful as biomarkers in determining the toxic effects in O. mykiss exposed to the RBBR textile dye.
AtıfScopus2İncele - MakaleAçık Erişim2016Scopus SJRQ1The in vitro effect of cypermethrin on quality and oxidative stress indices of rainbow trout Oncorhynchus mykiss spermatozoa
Kutluyer, Filiz; Benzer, Fulya; Erisir, Mine; Ögretmen, Fatih; Inanan, Burak Evren
There is limited information on the scientific literature about the effect of in vitro exposure of fish sperm to pesticides. In vitro effect of cypermethrin on sperm quality and oxidative stress has not yet been fully investigated. Therefore, the effects of cypermethrin, a type II pyrethroid insecticide, on quality and oxidative stress of spermatozoa were examined in vitro. To explore the potential in vitro toxicity of cypermethrin, fish spermatozoa were incubated with different concentrations of cypermethrin (1.025, 2.05 and 4.1 mu g/l) for 2 h. The motility rate and duration of sperm were determined after exposure to cypermethrin. Reduced glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT) and malondialdehyde (MDA) in spermatozoa were analyzed for determination of oxidant and antioxidant balance. Our results indicated that spermatozoa motility and duration significantly decreased with exposure to cypermethrin. Additionally, activity of GSH-Px (P < 0.05) and MDA and GSH levels increased in a concentration-dependent manner while CAT activity decreased (P < 0.05). Consequently, the oxidant and antioxidant status and sperm quality were affected by quantitative changes and different concentrations of cypermethrin. (C) 2015 Elsevier Inc. All rights reserved.
AtıfWoS35OpenAlex38İncele - MakaleYalnız künye2015Scopus SJRQ3ANTIOXIDANT RESPONSES OF RAINBOW TROUT (Oncorhynchus mykiss) GILLS AFTER EXPOSURE TO HYDROGEN PEROXIDE
Seker, Engin; Ispir, Unal; Yonar, Serpil Mise; Yonar, M. Enis; Turk, Cebrahil
We evaluated antioxidant responses of Oncorhynchus mykiss, experimentally exposed to different concentrations of hidrogen peroxide (H2O2). Different concentrations of H2O2 (50 and 100 ppm) were administrated to the fish for 30, 60 and 90 min. Samples of the gill were collected at the end of the experiment, and analysed for their oxidant antioxidant status, including the malondialdehyde (MDA) level, the catalase (CAT) and glutathione peroxidase (GSH-Px) activity as well as the reduced glutathione (GSH) concentration. Results obtained showed that H2O2 significantly increased MDA level. Compared to controls, GSH level and GSH-Px and CAT activities were significantly reduced in the fish that were exposed to different concentrations of H2O2. The current data suggest that H2O2 has an oxidative-stress-inducing potential in fish. The various parameters studied in this investigation can also be used as biomarkers of exposure to H2O2.
AtıfWoS18İncele - MakaleAçık Erişim2015Scopus SJRQ2The in vitro effect of Lambda-cyhalothrin on quality and antioxidant responses of rainbow trout Oncorhynchus mykiss spermatozoa
Kutluyer, Filiz; Erisir, Mine; Benzer, Fulya; Ögretmen, Fatih; Inanan, Burak Evren
There is little information in the scientific literature about effect of in vitro exposure of fish spermatozoa to pesticides. In vitro effect of Lambda-cyhalothrin (La) on sperm quality and oxidative stress has not been fully explored yet. The effects of LCT, which is a synthetic pyrethroid insecticide, on quality and oxidative stress of spermatozoa were investigated in vitro due to extensively use to control a wide range of insect pests in agriculture, public health, and homes and gardens. To explore the potential in vitro toxicity of La, fish spermatozoa were incubated with different concentrations of LCT (0.6, 1.2 and 2.4 mu g/L) for 2 h. Reduced glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT) and malondialdehyde (MDA) in spermatozoa were analyzed for determination of oxidant and antioxidant balance. Our results indicated that the percentage and duration of sperm motility significantly decreased with exposure to LCT. Activity of GSH-Px and MDA (P < 0.05) and GSH levels (P < 0.05) increased in a concentration-dependent manner while CAT activity decreased (P < 0.05). In conclusion, the oxidant and antioxidant status and sperm quality were affected by increasing concentrations of LCT. (C) 2015 Elsevier B.V. All rights reserved.
AtıfWoS26OpenAlex30İncele - MakaleYalnız künye2014Scopus SJRQ1Effect of ellagic acid on some haematological, immunological and antioxidant parameters of rainbow trout (Oncorhynchus mykiss)
Yonar, S. Mise; Yonar, M. E.; Yöntürk, Y.; Pala, A.
In this study, effect of ellagic acid on some haematological, immunological and antioxidant parameters in the blood and various tissues of rainbow trout (Oncorhynchus mykiss) were examined. Four groups of rainbow trout were fed experimental diets containing either no ellagic acid (control) or supplemented with ellagic acid at 50mg/kg diet (EA-50), 100mg/kg diet (EA-100) or 150mg/kg diet (EA-150) for 21days. Samples of the blood and tissue (liver, kidney and spleen) were collected at the end of the experiment and analysed for their haematological profile (the red blood cell count, the haemoglobin concentration and the haematocrit level), immune response (the white blood cell count, the oxidative radical production (NBT activity), the total plasma protein and total immunoglobulin level) and oxidant/antioxidant status (the malondialdehyde level, the superoxide dismutase, catalase and glutathione peroxidase activity as well as the reduced glutathione concentration). The findings of this study demonstrated that ellagic acid had a positive effect on the haematological parameters, the immune response and the antioxidant enzyme activities of the fish.
AtıfWoS35OpenAlex37İncele - MakaleAçık Erişim2014Scopus SJRQ1Cryopreservation of rainbow trout Oncorhynchus mykiss spermatozoa: Effects of extender supplemented with different antioxidants on sperm motility, velocity and fertility
Kutluyer, Filiz; Kayim, Murathan; Ögretmen, Fatih; Büyükleblebici, Serhat; Tuncer, P. Barbaros
In present study, it was examined whether addition of different antioxidants to the cryopreservation extenders had an effect on semen post-thaw fertility and motility in rainbow trout (Oncorhynchus mykiss) and also it was investigated the sperm characteristics post-thaw sperm characteristics and fertility. The collected semen was pooled to minimize individual variation. Each pooled ejaculate was split into 12 equal aliquots and diluted with base extenders supplemented with the antioxidants, and a base extender with no additives (control). The pooled semen samples diluted at the ratio of 1:10 by the extenders were subjected to cryopreservation. Antioxidants were separately added to the extenders (one per experimental group): catalase (250 U/l), superoxide dismutase (250 U/l), peroxidase (250 U/l), oxidized glutathione (1.5 mmol/l), reduced glutathione (1.5 mmol/l), L-methionine (1.5 mmol/l), uric acid (0.25 mmol/l), L-ascorbic acid (0.5 mmol/l), alpha-tocopherol (2.0 mmol/l), beta-carotene (0.5 mmol/l) and camitine (0.5 mmol/l). After dilution the semen was aspirated into 0.25 ml straws, the straws were placed on the tray, frozen for 10 min, and plunged into liquid nitrogen. Our results indicated that the post-thaw motility rate increased in extenders supplemented with uric acid, L-methionine, SOD, L-carnitine, alpha-tocopherol and L-reduced glutathione (p < 0.05). The motility duration of frozen thawed semen increased in extenders supplemented with uric acid, L-methionine, SOD, alpha-tocopherol and L-reduced glutathione (p < 0.05). Fertilization rate and hatching rate of frozen-thawed semen was not affected by the tested antioxidants. Consequently, the tested antioxidants affected the motility parameters and cryopreservation extenders could be supplement with antioxidants. This study suggested usage of antioxidants in the cryopreservation of rainbow trout. (C) 2014 Elsevier Inc. All rights reserved.
AtıfWoS76OpenAlex92İncele - MakaleYalnız künye2013Scopus SJRQ3EFFECTS OF SEAWATER ACCLIMATIZATION ON GILL Na+-K+-ATPase ACTIVITIES AND CHLORIDE CELLS IN RAINBOW TROUT (Oncorhynchus mykiss) AND BROWN TROUT (Salmo trutta forma fario)
Kizak, V.; Ozden, O.; Guner, Yusuf
In this study, rainbow trout (Oncorhynchus mykiss) (162.7 +/- 3.03 g) and brown trout (Salmo trutta forma fario) (160.9 +/- 2.94 g) were transferred to full-strength seawater (36.5 gl(-1)) for directly and gradually (21 days), then changes in gill Na+-K+-ATPase activity and size of chloride cells associated with environmental salinity were investigated and also survival of trouts were evaluated in seawater. All fish died when brown trouts were transferred into seawater directly but rainbow trouts survived 50 - 58.3 %. However, significant difference was recorded between brown and rainbow trouts in terms of survival rates by gradual acclimation. Survival of brown trout and rainbow trout that were transferred in seawater gradually, was 66.7 - 75 % and 83.3 - 91.7 %, respectively (p < 0.05). Gill chloride cell sizes in both species increased at 36.5 gl(-1) salinity. The lowest sectional area of chloride cells was determined at the point of death in brown trouts which were transferred directly. Following direct transfer to seawater, the largest sectional area of chloride cells were determined in rainbow trout. There are significant differences between acclimated and non-acclimated fish in terms of chloride cell sizes and Na+-K+-ATPase activities (p < 0.05). The highest gill Na+-K+-ATPase activity was in brown trouts which were transferred gradually, while the lowest activity was detected in freshwater phase. According to the results, gradually acclimated trouts can perform better adaptation than their directly acclimated counterparts.
AtıfWoS1İncele - MakaleAçık Erişim2012Scopus SJRQ2Investigation of Yersinia ruckeri Infection in Rainbow Trout (Oncorhynchus mykiss Walbaum 1792) Farms by Polymerase Chain Reaction (PCR) and Bacteriological Culture
Seker, Engin; Karahan, Murat; Ispir, Unal; Çetinkaya, Burhan; Saglam, Naim; Sarieyyüpoglu, Mustafa
This study has been carried out to investigate the presence of Yersinia ruckeri, the causative agent of Enteric Red Mouth (ERM) disease, in rainbow trout fish breeding farms located in Elazig and Malatya provinces in Eastern Turkey. For this purpose, bacteriological culture and a specific polymerase chain reaction (PCR) have been compare with examine blood and intestine samples collected from adult and young rainbow trout fish as well as water samples collected from all the farms. Y. ruckeri was isolated and identified from eight of the 17 fish farms giving a proportion of 52.9% at fish farm. The disease was noted in five of the rainbow trout farms located in Elazig and three of the 11 rainbow trout farms located in Malatya. The agent was detected in five of the six young fish breeding farms. The isolation percentages of the causative agent at fish level were calculated as 25.7% (131/510) in adult and 31.7% (19/60) in young fish, giving an overall proportion of 26.3% (150/570). All the isolates have been successfully identified as Y ruckeri by species-specific PCR. No isolation could be made from water samples. It is therefore concluded that Y. ruckeri infection poses a significant threat to the fish farms in the study area and necessary precautions should urgently be taken in order to minimize economical losses due to ERM disease. A specific PCR can be utilized successfully in aquaculture for rapid identification of bacterial agents which will help to prevent spread of infectious diseases and will therefore contribute to the productivity of fishery sector.
AtıfWoS6OpenAlex7İncele - MakaleAçık Erişim2011Scopus SJRQ3Comparison of the survival and growth performance in rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta fario) fry
Kizak, Volkan; Guner, Yusuf; Turel, Murat; Can, Erkan; Kayim, Murathan
Rainbow trout is an intensively cultured species because of their more cultivable character than brown trout. Culture of brown trout could not be expanded due to low growth performance of brown trout compared to rainbow trout. This experiment was conducted in a commercial trout farm, Aegean area (Turkey). Growth performances and survivals of rainbow and brown trouts from fry to fingerling was observed for 155 days. Growth performances, feed conversion rates (FCR) and survivals were determined. Initial weights of rainbow and brown trouts were 0.1 +/- 0.01 g. Final weights were 26.5 +/- 5.19 and 12.97 +/- 2.74 g, respectively at the end of the experiment. Survival and FCR of rainbow and brown trouts were 83.9, 80 and 0.59, 0.61% respectively. As a result, there is a similarity between these two trout species in point of survivals and FCR's although, growth performance was obviously better than brown trout in early development of rainbow trout.
AtıfWoS5OpenAlex16İncele - MakaleYalnız künye2011Scopus SJRQ3A comparison of the survival and growth performance in rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta fario) fry
Kizak, Volkan; Guner, Yusuf; Turel, Murat; Can, Erkan; Kayim, Murathan
The rainbow trout is an intensively cultured species because of its more cultivatable character than the brown trout. In this experiment, the brown trout culture did not expand due to low growth performance compared to that of the rainbow trout. This experiment was conducted in a commercial trout farm, Aegean Area (Turkey). Growth performances and survivals of rainbow and brown trouts from fry to fingerling were observed for 155 days. Growth performances, feed conversion rates and survivals were determined. The initial weights of the rainbow and brown trouts were 0.1 +/- 0.01 g. Final weights were 26.5 +/- 5.19 g and 12.97 +/- 2.74 g, respectively at the end of experiment. Survival and FCR of rainbow and brown trouts were 83.9 and 80%; 0.59 and 0.61 respectively. As a result, there is a similarity between these two trout species in point of survivals and FCR's although growth performance of the rainbow trout was significantly better than that of the brown trout.
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