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• MakaleYalnız künye2011Scopus SJRQ3/r/munzur/4088
  DETECTION OF YERSINIA RUCKERI BY POLYMERASE CHAIN REACTION (PCR) IN INFECTED RAINBOW TROUT (ONCORHYNCHUS MYKISS WALBAUM 1792)

  Seker, E.; Karahan, M.; Sarieyyüpoglu, M.; Çetinkaya, B.

  This study was conducted to induce experimental infection in rainbow trout with Y. ruckeri, the causative agent of ERM disease and progress of the disease was then monitored over the time period. Application of PCR for early and accurate diagnosis of infection was also investigated. Total 100 fish were allocated to four experimental and a control group each containing 20 fish. There were 20 aquaria and five fish were housed in each aquaria. There were 4 replicates for each group. Fish, at an average weight of 75 g, were injected intramuscularly with 0.1 ml dilutions of Y. ruckeri at various doses (4x10(8), 1x10(8), 4x10(4) and 1x10(4) per ml) in the experimental groups. Blood samples were collected from fish that showed typical signs of the disease. DNA's extracted from blood samples were subjected to PCR amplification based upon a pair of Y. ruckeri specific primers. Following agarose gel electrophoresis of the PCR products, positive bands with the molecular size of approximately 589 bp which were considered indicative for identification as Y. ruckeri were observed. In addition, isolation and identification of Y. ruckeri from various internal organs of infected fish were made by conventional culture method. The results suggested that early diagnosis of ERM disease can be made while fish are alive and diseased fish can be isolated and treated accordingly which will help decrease economic losses.

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• MakaleYalnız künye2011Scopus SJRQ2/r/munzur/3987
  Immunostimulating Effect of Levamisole on Spleen and Head-Kidney Leucocytes of Rainbow trout (Oncorhynchus mykiss, Walbaum 1792)

  Seker, Engin; Ispir, Unal; Dörücü, Mustafa

  An in vitro cultivation method for spleen and head kidney organ section were used to investigate the effects of levamisole on the immune response. After 10 days of culture with 10 mu g/ml, 50 mu g/ml and 100 mu g/ml or no levamisole in the culture media, the non specific defense reaction were measured by glass-adherent NBT (nitroblue tetrazolium) positive cells activation and phagocytic activity. Elevation in phagocytic activity was found for 10 mu g/ml and 50 mu g/ml levels of levamisole (P < 0.001), but no differences were found at levels of phagocytic activity in between 100 mu g/ml levamisole groups and control groups (P > 0.05). In the spleen and head kidney leucocytes, increased response to levamisole was shawn for all doses in the glass-adherent NBT positive cells activation.

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