Sürdürülebilir Kalkınma Amaçları

Yazarlar

  • MakaleYalnız künye2011Scopus SJRQ2
    Presence of Staphylococcus aureus and Staphylococcal Enterotoxins in Different Foods

    Koluman, Ahmet; Unlu, Teoman; Dikici, Abdullah; Tezel, Atanur; Akcelik, E. Nazan; Burkan, Zeynep T.

    Staphylococcal food poisoning is one of the most prevalent causes of gastroenteritis throughout the world. This study has three steps. In the first step of the study, different food matrices were analyzed for the presence of Staphylococcus aureus. In the second step, types of staphylococcal enterotoxins (SET) from these foods were analyzed. Lastly, antibiotic resistance of S. aureus isolated was determined using conventional and PCR methods. For this purpose, 300 different food samples were evaluated for contamination with S. aureus. From these samples 221 S. aureus strains were isolated. The same strains were evaluated for antibiotic resistance and existence of mecA gene. All strains were at least resistant to 2 antibiotics, but no MRSA strain was detected. Staphylococcal Enterotoxins (SE) were determined with VIDAS and ELISA from the foods of concern. The results of the analysis held with Vidas SET2 showed 72 (24%) of 300 samples and with ELISA these enterotoxins showed difference in distribution.

    AtıfWoS14
    Web of Scienceİncele
  • MakaleAçık Erişim2011Scopus SJRQ2
    Distribution of neuropeptides in endocrine and exocrine pancreas of long-legged buzzard (Buteo rufinus): An immunohistochemical study

    Bayrakdar, Ali; Yaman, Mine; Atalar, Omer; Tarakci, Berrin Gencer; Ceribasi, Songul

    This study aimed to determine the existence and distribution of certain neuropeptides in endocrine and exocrine pancreas of the long-legged buzzard by using immunohistochemical methods. SOM-14-, NPY- and CGRP-IR endocrine cells were determined in both central and peripheral regions in A-islets within the pancreas, while SP-IR endocrine cells were found only in the central region, and CCK-8- and galanin-IR endocrine cells were only detected in peripheral region. On the other hand, in B-islets; SP-, NPY- and CGRP-IR endocrine cells were determined in both central and peripheral regions, while SOM-14- CCK-8- and galanin-IR endocrine cells were found only in the peripheral region. In addition; SOM-14-, NPY-, CGRP-. CCK-8- and galanin-IR cells were also observed in exocrine pancreas. This distribution pattern in the pancreas of the long-legged buzzard demonstrates that neuropeptides perform their probable affects through endocrine and/or paracrine mechanisms. In conclusion, the existence and distribution of neuropeptides in the pancreas of long-legged buzzard have been introduced in this study for the first time and this bird species has also been found to differ from other types of avian species. (c) 2010 Elsevier B.V. All rights reserved.

    AtıfWoS11OpenAlex15
    Web of SciencePubMedİncele
  • MakaleYalnız künye2011Scopus SJRQ3
    Physico-chemical and Microbiological Water Quality Assessment of Perisuyu River, Tunceli, Turkey

    Kaplan, O.; Yildirim, N. C.; Yildirim, N.; Akyol, E. A.

    Generally, the water quality depends on its chemical and microbiological conditions. So, in this study, the chemical and microbiological characteristics of the Perisuyu River through 4 year long surveillance in Tunceli. Turkey were studied. Sampling was carried out four times during the year. Nitrate, nitrite, ammonium concentrations, total dissolved solid, total coliform counts were determined and the presence of Escherichia coli, important water quality indicator, was tested. Generally in water samples E. coli and total coliform were found very high and nitrate, nitrite and ammonium concentrations were low. In 2008 August and 2009 February water pH was higher than the World Health Organization limit value. Electrical conductivity was higher in 2004 May, December and 2008 February.

    AtıfWoS3
    Web of Scienceİncele
  • MakaleAçık Erişim2011Scopus SJRQ3
    Comparison of the survival and growth performance in rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta fario) fry

    Kizak, Volkan; Guner, Yusuf; Turel, Murat; Can, Erkan; Kayim, Murathan

    Rainbow trout is an intensively cultured species because of their more cultivable character than brown trout. Culture of brown trout could not be expanded due to low growth performance of brown trout compared to rainbow trout. This experiment was conducted in a commercial trout farm, Aegean area (Turkey). Growth performances and survivals of rainbow and brown trouts from fry to fingerling was observed for 155 days. Growth performances, feed conversion rates (FCR) and survivals were determined. Initial weights of rainbow and brown trouts were 0.1 +/- 0.01 g. Final weights were 26.5 +/- 5.19 and 12.97 +/- 2.74 g, respectively at the end of the experiment. Survival and FCR of rainbow and brown trouts were 83.9, 80 and 0.59, 0.61% respectively. As a result, there is a similarity between these two trout species in point of survivals and FCR's although, growth performance was obviously better than brown trout in early development of rainbow trout.

    • SDG 2 · Açlığa Son
    AtıfWoS5OpenAlex16
    Web of Scienceİncele
  • MakaleYalnız künye2011Scopus SJRQ3
    Levels of copper in liver, muscle and gill tissues in Capoeta trutta (Heckel, 1843) from Munzur River, Turkey

    Danabas, D.; Benzer, F.; Kaplan, O.; Yildirim, N. C.

    Heavy metal pollution of waters is a major environmental problem. Due to their toxicity, long persistence, bioaccumulative and nonbiodegradable properties in the food chain, heavy metals constitute a core group of aquatic pollutants. Capoeta trutta is an economically important fish and the object of significant commercial fishery in the Munzur River. Levels of Cu were measured in samples of the muscle, gill and liver tissues of C. trutta from Munzur River. In liver copper levels were higher at Station I (6.15 mg kg(-1)) polluted by domestic waste than Station II (5.48 mg kg(-1)) (P<0.05). The highest copper level (7.25 mg kg(-1)) was measured in muscle tissue at Station II. There was found no statically differences between stations in gill and muscle (P>0.05). It is suggested that ecological condition of freshwater fish and contamination levels in Munzur River must be regularly monitored.

    AtıfWoS7
    Web of Scienceİncele
  • MakaleYalnız künye2011Scopus SJRQ2
    The Effectiveness of Pleurotus eryngii Strains in Biodelignification of Cotton Stalk, Causing Environmental Hazard and Disposable Problem

    Yildirim, Numan; Yildiz, Abdunnasir

    Biodelignification of cotton stalk (CS) by Pleurotus eryngii collected from Tunceli and Elazig province of Turkey under solid state fermentation (SSF) was studied. The effect of the addition of varying concentration (5 & 10% w/w) of rice bran (RB) on lignin degradation and Carbon/Nitrogen (C/N) ratio were investigated. Klason lignin (KL) degradation was followed for 140 day. While substrate initially contained 28.66% KL, maximum lignin loss (69.68+/-1.05%), occurred on CS +5% RB, C/N ratio of non-fermented cotton stalk initially was 34.13+/-4.98% and after incubation this ratio decreased to 18.60+/-0.36% on CS without RB. It was concluded that P. eryngii strain collected from Tunceli province is very effective in delignifying cotton stalk. (C) 2011 Friends Science Publishers

    AtıfWoS2
    Web of Scienceİncele
  • MakaleYalnız künye2011Scopus SJRQ3
    A comparison of the survival and growth performance in rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta fario) fry

    Kizak, Volkan; Guner, Yusuf; Turel, Murat; Can, Erkan; Kayim, Murathan

    The rainbow trout is an intensively cultured species because of its more cultivatable character than the brown trout. In this experiment, the brown trout culture did not expand due to low growth performance compared to that of the rainbow trout. This experiment was conducted in a commercial trout farm, Aegean Area (Turkey). Growth performances and survivals of rainbow and brown trouts from fry to fingerling were observed for 155 days. Growth performances, feed conversion rates and survivals were determined. The initial weights of the rainbow and brown trouts were 0.1 +/- 0.01 g. Final weights were 26.5 +/- 5.19 g and 12.97 +/- 2.74 g, respectively at the end of experiment. Survival and FCR of rainbow and brown trouts were 83.9 and 80%; 0.59 and 0.61 respectively. As a result, there is a similarity between these two trout species in point of survivals and FCR's although growth performance of the rainbow trout was significantly better than that of the brown trout.

    AtıfWoS4
    Web of Scienceİncele
  • MakaleYalnız künye2011Scopus SJRQ3
    Plasma nitric oxide concentrations and erythrocyte arginase activities in lambs with contagious ecthyma

    Kandemir, F. M.; Issi, M.; Benzer, F.; Gül, Y.; Basbug, O.; Özdemir, N.

    The aim of the present study was to investigate the plasma nitric oxide concentrations and the erythrocyte arginase activities in lambs with contagious ecthyma, which frequently occurs in lambs and goat kids. The study was conducted on 10 Akkaraman, 1-2 month old lambs with contagious ecthyma (the clinical diagnosis was based on the presence of typical cauliflower-like proliferative lesions in lips, commissum labials, nostrils and udders) and on 10 apparently healthy lambs (controls) from the same flock. Plasma NO concentrations and erythrocyte arginase activities were determined on all animals using the Griess method and the thiosemicarbazide diacetylmonoxime urea (TDMU) method, respectively. Compared to the control group, the mean plasma NO concentration was significantly increased in the diseased group while the mean arginase activity was markedly depressed. Abnormally high NO concentrations and low enzyme activities were observed in 9 and 10 diseased lambs, respectively. Additionally, a negative correlation was evidenced between the 2 biochemical parameters. These results show that this viral infection is associated with some perturbations in the arginine metabolism, leading preferentially to the synthesis of NO, which is presumed to exhibit some antiviral properties.

    AtıfWoS7
    Web of Scienceİncele
  • MakaleYalnız künye2011Scopus SJRQ3
    Synthesis and Characterization of Ni(II) and Cd(II) Complexes of 4-(4-Nitrophenylazo)-1H-pyrazole-3,5-diamine and 4-(4-Methylphenylazo)-1H-pyrazole-3,5-diamine

    Adiguzel, Ragip; Esener, Hasan; Ergin, Zuhal; Aktan, Ebru; Turan, Nevin; Sekerci, Memet

    In this study, the diazotized p-nitroaniline and p-methylaniline were coupled with malononitrile. The ring closure reaction of the obtained products with hydrazine monohydrate yielded 4-(4-methylphenylazo)-1H-pyrazole-3,5-diamine (L-1) and 4-(4-nitrophenylazo)-1H-pyrazole-3,5-diamine (L-2). The structure of the ligands has been elucidated by spectroscopic analyses. Then, novel Ni(II) and Cd(II) complexes of the ligands have been synthesized and the structures of these complexes determined by elemental analysis, spectrometric and TGA/DTA methods, molar conductance and magnetic susceptibility measurements. All the complexes were monomeric and diamagnetic. From the elemental analyses and mass spectra data, the complexes were proposed to the formulae [Ni-2(L-1)(2)(OH)(2)]center dot 2Cl center dot 8.5H(2)O, [Cd-2(L-1)(2)(OH)(2)]center dot 2Cl center dot DMF center dot 3H(2)O, [Ni(L-2)(2)]center dot 2Cl center dot 2DMF center dot 7H(2)O and [Cd-2(L-2)(2)(OH)(2)(H2O)(4)]center dot 2Cl center dot 1.5H(2)O. For the Cd(L-2) complex octahedral geometry was proposed, but the Ni(L-1), Ni(L-2) and Cd(L-1) complexes show four coordinated structure. The Ni(L-1), Cd(L-1) and Cd(L-2) complexes were found to be dinuclear. On the other hand the Ni(L-2) complex was found to be mononuclear. All the complexes were found to be (1:2) electrolytes.

    AtıfWoS8
    Web of Scienceİncele
  • MakaleAçık Erişim2011Scopus SJRQ2
    Synthesis and Characterization of Novel Mn(II), Co(III), Ni(II) and Cd(II) Complexes from 4-(2-nitrophenylazo)-1H-pyrazole-3,5-diamine

    Esener, Hasan; Adiguzel, Ragip; Ergin, Zuhal; Aktan, Ebru; Turan, Nevin; Sekerci, Memet

    In this study, the diazotized o-nitroaniline was coupled with malononitrile. The ring closure reaction of the obtained product with hydrazine monohydrate yielded 4-(2-nitrophenylazo)-1H-pyrazole-3,5-diamine (L). The structure of L has been elucidated by spectroscopic analyses. Then, novel Mn(II), Co(II), Ni(II) and Cd(II) complexes of L have been synthesized, and the structures of these complexes determined by elemental analysis, spectrometric and TGA/DTA methods, molar conductance and magnetic susceptibility measurements. All complexes were monomeric. Except Mn(II) complex, other complexes were diamagnetic. From the elemental analyses and mass spectra data, the complexes were proposed to the formulas [MnL2(CH3O)H2O]center dot Cl center dot CH3OH, [CoL2(CH3O)(2)]center dot Cl center dot 2.5CH(3)OH, [NiL2(CH3OH)(2)]center dot 6.5CH(3)OH and [Cd2L2(Cl)(2)(CH3O)(2)]center dot CH3OH center dot 1.5H(2)O. For Mn(II) and Co(III) complexes octahedral and for the Ni(II) complex square planar geometry were proposed, but the Cd(II) complex includes five coordinated structure. The Mn(II), Co(II) and Ni(II) complexes were found to be mononuclear and electrolytes. On the other hand Cd(II) complex was found to be dinuclear and non-electrolyte.

    AtıfWoS12OpenAlex9
    Web of Scienceİncele
  • MakaleYalnız künye2011Scopus SJRQ3
    Effect of Pomegranate Seed Extract on Free Radical Damage and Antioxidant Activity Under Cisplatin-Induced Oxidative Stress Conditions in Rabbit Testes

    Benzer, F.; Kandemir, F. M.; Yildirim, N. C.; Ozan, S. T.

    The aim of this study is to evaluate the protective effect of pomegranate seed extract on cisplatin-induced oxidative stress in rabbits testes. Rabbits were divided into three groups; (i) being control group, (ii) cisplatin group and (iii) cisplatin + pomegranate seed extract group. The level of malondialdehyde and glutathione, the activity of catalase, glutathione peroxidase, were measured in testes tissue of rabbits. Malondialdehyde levels were increased depending on cisplatin. In the case of cisplatin + pomegranate seed extract, malondialdehyde levels were found to be lower than cisplatin group (p < 0.05). In cisplatin group, catalase, glutathione peroxidase activity and glutathione levels were decreased when compared to control but in cisplatin + pomegranate seed extract group, catalase, glutathione peroxidase activity and glutathione levels were found higher than cisplatin group (p < 0.05). The pomegranate seed extract supplementation may play a protective role or decrease the side effects of cisplatin-induced testes toxicity from cancer medicines.

    AtıfWoS5
    Web of Scienceİncele
  • MakaleYalnız künye2011Scopus SJRQ2
    Compensatory effects of curcumin on cisplatin-induced toxicity in rabbit testis

    Kandemir, F. M.; Benzer, F.; Yildirim, N. C.; Ozdemir, N.

    Curcumin, a widely used spice and colouring agent in food, has been shown to possess potent antioxidant, antitumor promoting and anti-inflammatory properties in vitro and in vivo. The present study was designed to investigate the protective effects of curcumin on changes in the levels of lipid peroxidation and endogenous antioxidants induced by cisplatin (cis-diamminedichloroplatinum II, CDDP) in the testiclular tissue of rabbits. 18 healthy male New Zealand white rabbits were equally divided into three groups of six rabbits each, control, cisplatin, and cisplatin+curcumin. The degree of protection produced by cisplatin was evaluated by determining the level of malondialdehyde (MDA) and glutathione (GSH), the activity of catalase (CAT), glutathione peroxidase (GSH-Px), were estimated from testes homogenates. MDA levels were increased with cisplatin compared to control but in cisplatin+curcumin group, MDA levels were found to be lower than cisplatin group (p<0.05). The activity of CAT and GSH-P-X was decreased in cisplatin and cisplatin+curcumin groups compared to control (p<0.05). In the case of cisplatin+curcumin CAT and GSH-Px activity were increased compared to cisplatin group (p<0.05). GSH levels were decreased with cisplatin but administration cisplatin+curcumin increased the levels of GSH comared to cisplatin group (p<0.05). In the present study, co-administration of curcumin with cisplatin prevented the damage to testes induced by this drug and may be considered as a potentially useful candidate in the combination chemotherapy with cisplatin.

    AtıfWoS22
    Web of Scienceİncele
  • MakaleYalnız künye2011Scopus SJRQ3
    MALONDIALDEHYDE LEVELS AND GLUTATHIONE PEROXIDASE ACTIVITIES IN THE TISSUES OF FRESHWATER CRAYFISH (ASTACUS LEPTODACTYLUS ESCH 1823) IN CULTURE CONDITION

    Kandemir, F. M.; Benzer, F.; Erisir, M.; Yildirim, N. C.

    The objective of the study was to characterize the effects of sex on lipid peroxidation (as malondialdehyde, MDA), antioxidant defence system (as glutathione peroxidase, GSH-Px) in the hepatopancreas, gonad and muscle tissues of freshwater crayfish, Astacus leptodactylus, in culture condition. The crayfish used in the present study was provided from Keban Dam Lake population of A. leptodactylus. 34 males and 30 females crayfish in different size were hold in stock ponds (6 x 6 x 0.5 m). This study was carried out between June 18, 2004 and December 1, 2004 at the crayfish reproduction unit of Aquaculture Faculty of Firat University, Elazig, Turkey. Plastic pipes (20 cm in length and 7 cm in diameter) were provided as shelters for the crayfish. Crayfish were fed 2 % of their total wet weight daily with prepared ration. Crayfish were exposed to natural photoperiod. During the study, the mean water temperature, dissolved oxygen and mean pH were 4 degrees C, 6.28 +/- 0.32 mg L-1 and 7.82 +/- 0.17, respectively. MDA and GSH-Px levels according to sex and tissue-species were determined. MDA level in the hepatopancreas and gonad of females were higher than males, except muscle tissue (P<0.001). However, the GSH-Px activities in the hepatopancreas and gonad of males were higher than females, except muscle tissue (P<0.001). As a result, MDA and GSH-Px levels in the hepatopancreas, gonad and muscle tissues of freshwater crayfish, Astacus leptodactylus, in culture condition changed both tissues and sex.

    AtıfWoS4
    Web of Scienceİncele
  • MakaleYalnız künye2011Scopus SJRQ2
    Antibacterial Effects of Some Fermented Commercial and Homemade Dairy Products and 0.9% Lactic Acid against Selected Foodborne Pathogens

    Yesillik, S.; Yildirim, N.; Dikici, A.; Yildiz, A.

    In this study, the antibacterial effects of homemade yoghurt, commercial yoghurt, commercial kefir and probiotic yoghurt on viability of Salmonella typhimurium (ATCC 14028), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923) was investigated by using disc diffusion method. Antibaterial effects of the samples were tested at 24, 48 h and the 7 day intervals. Homemade yoghurt showed the maximum antibacterial effect against the pathogens. It was determined that the most sensitive pathogenic bacteria to dairy products were Salmonella typhimurium whereas the least sensitive pathogen was Pseudomonas aeruginosa. Comparing to the antimicrobial effect of 0.9% lactic acid which was used as positive control, our results concluded that the bacteria found in fermented dairy products have an antibacterial activity and this activity is not caused by only lactic acid.

    • SDG 2 · Açlığa Son
    • SDG 3 · Sağlık ve Kaliteli Yaşam
    AtıfWoS23OpenAlex38
    Web of Scienceİncele
  • MakaleYalnız künye2011Scopus SJRQ3
    Synthesis and Characterization of Novel Ni(II), Cu(II) and Cd(II) Complexes of 4-(2-Chlorphenylazo)-1H-pyrazole-3,5-diamine

    Adiguzel, Ragip; Esener, Hasan; Ergin, Zuhal; Aktan, Ebru; Sekerci, Memet

    In this study, the diazotized o-chloraniline was coupled with malononitrile. The ring closure reaction of the obtained product with hydrazine monohydrate yielded 4-(2-chlorophenylazo)-1H-pyrazole-3,5-diamine (L). The structure of ligand has been elucidated by spectroscopic analyses. Then, novel Ni(II), Cu(II), Cd(II) complexes of ligand have been synthesized and the structures of these complexes determined by elemental analysis, spectrometric and TGA/DTA methods and magnetic susceptibility measurements. All complexes were monomeric and diamagnetic. From the elemental analyses and mass spectra data, the complexes were proposed to the formulae [Ni2L2(Cl)(2)]center dot 2Cl center dot 0.5DMF center dot 0.5H(2)O(2), [Cu3L2(Cl)(4)(OH)(2)(H2O)(2)]center dot 0.5H(2)O and [Cd3L2(Cl)(2)(OH)(2)](Cl)(2)center dot 1.5H(2)O. For the Ni(II) complex square planar and for the Cu(II) complex octahedral geometry were proposed, but the Cd(II) complex includes both four and six coordinated structures. The Cu(II) and the Cd(II) complexes were found to be trinuclear. On the other hand the Ni(II) complex was found to be dinuclear.

    AtıfWoS7
    Web of Scienceİncele
  • MakaleYalnız künye2011Scopus SJRQ3
    Synthesis and Structural Characterization of Bis(2-amino-1,3,4-thiadiazolyl)methane Complexes

    Adiguzel, Ragip; Ergin, Zuhal; Sekerci, Memet; Tascioglu, Senay

    In this study, some transition metal complexes of the bis(2-amino-1,3,4-thiadiazolyl)methane (L) derived from the condensation of malonic acid and thiosemicarbazide, have been synthesized and characterized by IR, H-1-NMR spectra, elemental analyses, magnetic susceptibility and thermogravimetry-differential thermal analysis (TGA-DTA). Coordination numbers of monomeric, Ni(II), Zn(II), and Cd(II) complexes and dimeric Cu(II) complex were found to be four, five, six, and five, respectively. According to the elemental analyses and mass spectra data, the following complex formulas were proposed;[Ni-2(L)(SO4)(2)(H2O)(2)]center dot CH3OH center dot 0.5H(2)O, [Cu(L)(SO4)(H2O)](2)DMF center dot 3H(2)O, [Zn(L)NO3 center dot H2O]center dot NO3 center dot DMF, and [Cd(L)(2)(NO3)H2O]NO3 center dot DMF. Ni(II) and Cu(II) complexes were paramagnetic. Ni(II), Cu(II), and Cd(II) complexes were proposed to be tetrahedral/square planar, square pyramidal, and octahedral structure, respectively. Zn(II) complex is five coordinated. The molar conductance data revealed that Zn((II), and Cd(II) complexes were 1:1 electrolytes(ionic). Ni(II), and Cu(II) complexes were non-electrolytes.

    AtıfWoS6
    Web of Scienceİncele
  • MakaleAçık Erişim2011Scopus SJRQ2
    Sequential extraction of cadmium in different soil phases and plant parts from a former industrialized area

    Kaplan, Olcay; Ince, Muharrem; Yaman, Mehmet

    Cd concentrations in mobile phases of soil are more representative than total Cd concentration for estimating Cd bioavailability, physicochemical reactivity and mobility. In this study, selective sequential extraction procedures were used to determine Cd in different soil phases. Soil samples and plants grown in these soils were collected from a serpentine and copper-mining area in Maden-Elazig-Turkey. The extracted fractions were exchangeable/carbonate, reducible-iron/manganese oxides, oxidizable-organic matter and sulfides, and residual phases except silicates. Concentrations of Cd in soils and plant samples were determined by flame atomic absorption spectrometry and inductively coupled plasma-mass spectrometry. We found that Cd concentrations in the EDTA and NH(2)OH center dot HCl extracts are higher in most soil samples compared to the other extracts. We conclude that Cd levels in mobile phases are unexpectedly high. The observed Cd concentrations are in ranges of 0.03-3.4 mg kg(-1) for soil and 0.02-2.5 mg kg(-1) for plant parts. The percentages of cadmium up to 56% in exchangeable and carbonates fractions were observed to be significantly higher than in those values less than 2% reported in literature. This study has shown that the modified extraction method can be usefully applied to determine Cd concentrations in potentially mobile phase of soil. Furthermore, it was concluded that Brassicasea and Rumex leaves can be used as hyperaccumulator plants because their translocation factor and/or enrichment coefficient values were found to be higher than 1.0.

    AtıfWoS29OpenAlex29
    Web of Scienceİncele
  • MakaleYalnız künye2011Scopus SJRQ2
    BENEFICIAL EFFECTS OF GRAPE SEED EXTRACT AGAINST CISPLATIN-INDUCED TESTICULER DAMAGE IN RABBITS

    Yildirim, Nuran Cikcikoglu; Kandemir, Fatih Mehmet; Benzer, Fulya

    Biological compounds with antioxidant properties may contribute to the protection of cells and tissues against deleterious effects of reactive oxygen species (ROS) and other free radicals induced by cisplatin. This study was performed to investigate the possible protective role of antioxidant treatment with grape seed extract (GSE) on cisplatin-induced testes oxidant injury using biochemical approaches. The degree of protection produced by GSE was evaluated by determining the level of malondialdehyde (MDA) and glutathione (GSH), the activity of catalase (CAT), glutathione peroxidase (GSH-Px), were estimated from testes of rabbits. Male New Zealand white rabbits were divided into 3 groups (n=6): 1-Control group(1 ml saline. i.p) 2-Cisplatin group (a single dose of cisplatin (5 mg/kg, i.p) 3-A single dose of cisplatin (5 mg/kg, i.p) + GSE (250 mg/kg/day, i.p) for 6 days. MDA level were significantly higher when compared to Control (p < 0.05). In Cisplatin+grapes groups MDA levels were found significantly lower than cisplatin group (p < 0.05). GSH levels were decreased with cisplatin compared to control but in case of cisplatin+ GSE it increased (p < 0.05). In treated rabbits, the activity of CAT and GSH-Px was decreased in cisplatin and cisplatin+ GSE groups compared to control (p < 0.001). These results indicate that the antioxidant GSE might have a protective effect against cisplatin-induced testiculer damage and oxidative stress in rabbit.

    AtıfWoS12
    Web of Scienceİncele
  • MakaleYalnız künye2011Scopus SJRQ3
    DETECTION OF YERSINIA RUCKERI BY POLYMERASE CHAIN REACTION (PCR) IN INFECTED RAINBOW TROUT (ONCORHYNCHUS MYKISS WALBAUM 1792)

    Seker, E.; Karahan, M.; Sarieyyüpoglu, M.; Çetinkaya, B.

    This study was conducted to induce experimental infection in rainbow trout with Y. ruckeri, the causative agent of ERM disease and progress of the disease was then monitored over the time period. Application of PCR for early and accurate diagnosis of infection was also investigated. Total 100 fish were allocated to four experimental and a control group each containing 20 fish. There were 20 aquaria and five fish were housed in each aquaria. There were 4 replicates for each group. Fish, at an average weight of 75 g, were injected intramuscularly with 0.1 ml dilutions of Y. ruckeri at various doses (4x10(8), 1x10(8), 4x10(4) and 1x10(4) per ml) in the experimental groups. Blood samples were collected from fish that showed typical signs of the disease. DNA's extracted from blood samples were subjected to PCR amplification based upon a pair of Y. ruckeri specific primers. Following agarose gel electrophoresis of the PCR products, positive bands with the molecular size of approximately 589 bp which were considered indicative for identification as Y. ruckeri were observed. In addition, isolation and identification of Y. ruckeri from various internal organs of infected fish were made by conventional culture method. The results suggested that early diagnosis of ERM disease can be made while fish are alive and diseased fish can be isolated and treated accordingly which will help decrease economic losses.

    AtıfWoS3
    Web of Scienceİncele
  • MakaleYalnız künye2011Scopus SJRQ1
    Petrology and petrogenesis of carbonatitic rocks in syenites from central Anatolia, Turkey

    Cooper, Alan F.; Boztug, Durmus; Palin, J. Michael; Martin, Candace E.; Numata, Mihoko

    The late Cretaceous A-type Kara double dagger ayA +/- r pluton in Central Anatolia, Turkey, intrudes and entrains xenoliths of Palaeozoic limestone. Carbonatitic magmatic rocks within the syenite have been previously interpreted (Schuiling in Nature, 192:1280, 1961) to result from metasomatic alteration and syntectic melting of marble. Carbonatites and associated calcite-syenites exhibit mineralogical characteristics (Ab-rich plagioclase, Ba-rich K-feldspar, low-Mg# biotite) that are petrogenetically more evolved than the host syenitic suite. Geochemically, carbonate-rich magmatic rocks are greatly enriched in Sr, Ba, Th, and REE and have higher LREE/HREE ratios than either syenites or marbles. In terms of O-C-Sr-Pb isotope ratios, the carbonatite/calcite-syenite suite form a consistent and geochemically coherent group that is distinct from the marble country rock and xenolith population, but similar to some of the syenitic, and particularly the nepheline syenite components of the Kara double dagger ayA +/- r pluton. Other silicate magmatic rocks are geochemically, isotopically, and geochronologically different, suggesting the pluton is composite. Overall, the mineralogical and geochemical characteristics of the carbonatites are incompatible with binary mixing of syenite and marble but are consistent with derivation of carbonatite from petrogenetically evolved foid syenite. Carbonate-silicate rock types have modal variations compatible with an origin by fractional crystallisation, rather than by liquid immiscibility.

    AtıfWoS17OpenAlex17
    Web of Scienceİncele