Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Zengin, Gokhan(26)
- Erten, Fusun(20)
- Orhan, Cemal(17)
- Sahin, Kazim(17)
- Gul, Muhammet(15)
- Benzer, Fulya(12)
- Kocabaş, Mehmet(12)
- Aglar, Erdal(11)
- Serdar, Osman(11)
- Akkurt, Nevzat(10)
- Topal, Murat(10)
- Yeşiltepe, Akgün(10)
- Yucesan, Melih(10)
- Danabas, Durali(9)
- Er, Besir(9)
- Kutlu, Banu(9)
- Mahomoodally, Mohamad Fawzi(9)
- Uba, Abdullahi Ibrahim(9)
- Yildirim, Ozal(9)
- Öbek, Erdal(9)
- Acharya, U. Rajendra(8)
- Alp Dal, Nursel(8)
- Cakilcioglu, Ugur(8)
- Kandemir, Fatih Mehmet(8)
- Yildirim, Nuran Cikcikoglu(8)
- Arslan Topal, E. Işıl(7)
- Baloglu, Ulas Baran(7)
- Ozbey, Ulku(7)
- Sahin, Nurhan(7)
- Talo, Muhammed(7)
- Ates, Mehmet(6)
- Attar, Rukset(6)
- Dal, Nursel Alp(6)
- Gümüşsoy, Süreyya(6)
- Gürarslan Baş, Nazan(6)
- Ince, Muharrem(6)
- Kaplan Serin, Emine(6)
- Kutluyer, Filiz(6)
- Ozturk, Burhan(6)
- Aksu, Onder(5)
Yayınlar
- MakaleYalnız künye2021Scopus SJRQ1Protective Effects of Chrysin Against Oxidative Stress and Inflammation Induced by Lead Acetate in Rat Kidneys: a Biochemical and Histopathological Approach
Kucukler, Sefa; Benzer, Fulya; Yildirim, Serkan; Gur, Cihan; Kandemir, Fatih Mehmet; Bengu, Aydin Sukru; Ayna, Adnan; Caglayan, Cuneyt; Dortbudak, Muhammet Bahaeddin
In this study, the protective effects of chrysin (CR) on lead acetate (PbAc)-induced renal toxicity in Sprague-Dawley rats were investigated with biochemical, histopathological, and immunohistochemical methods. In the study, rats were given orally at 30 mg/kg/body weight (BW) PbAc after CR of 25 and 50 mg/kg/BW was administered to them orally (a total of 7 administrations for 7 days). The results showed that CR reduced urea and creatinine levels by alleviating PbAc-induced kidney damage. It was determined that CR decreases PbAc-induced lipid peroxidation due to its antioxidant properties and increases catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) activities, and glutathione (GSH) levels. It was also detected that CR protects DNA from the toxic effects of PbAc and reduces 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels. Biochemical and immunohistochemical findings demonstrated that CR had anti-inflammatory and antiapoptotic effects and reduced nuclear factor kappa-B (NF-κB), interleukin-33 (IL-33), prostaglandin-E2 (PGE-2), tumor necrosis factor-α (TNF-α), p53 levels, and the activities of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), which were increased with PbAc administration. Moreover, CR was found to increase the levels of aquaporin-1 (AQP-1) and nephrine in PbAc-induced kidney tissue. CR decreased the contents of lead (Pb), zinc (Zn), iron (Fe), sodium (Na), and copper (Cu) and increased those of potassium (K) calcium (Ca) in renal tissue. These results indicated that CR considerably alleviates kidney toxicity caused by PbAc.
AtıfScopus136WoS118OpenAlex145
İncele - MakaleAçık Erişim2021Scopus SJRQ1Chemical profiling and biological evaluation of nepeta baytopii extracts and essential oil: An endemic plant from turkey
Zengin, Gokhan; Mahomoodally, Mohamad Fawzi; Aktumsek, Abdurrahman; Jekő, József; Cziáky, Zoltán; Rodrigues, Maria João; Custodio, Luisa; Polat, Rıdvan; Cakilcioglu, Ugur; Ayna, Adnan
Nepeta baytopii is a poorly studied, endemic Nepeta species (Lamiaceae) of Turkey. For the first time, the biological activities (antioxidant, enzyme inhibition, and cytotoxicity properties) of the hexane, ethyl acetate, methanol, water/methanol, and water extracts and essential oil prepared from N. baytopii aerial parts were assessed. Hydro-methanol (41.25 mg gallic acid equivalent (GAE)/g) and water extracts (50.30 mg GAE/g), respectively showed the highest radical scavenging (94.40 and 129.22 mg Trolox equivalent (TE)/g, for 2,2-diphenyl-1-picrylhydrazyl radical and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid radical scavenging assays) and reducing (229.37 and 129.55 mg TE/g, for ferric-reducing antioxidant power and cupric-reducing antioxidant capacity assays) capacities in vitro. An interestingly high inhibition was observed for ethyl acetate extract against butyrylcholinesterase (10.85 mg galantamine equivalent/g). The methanol extract showed high cytotoxicity (31.7%) against HepG2 cells. Caryophyllene oxide was identified in high concentrations in the essential oil (39.3%). Luteolin and apigenin and their derivatives were identified from the methanol and water extracts. The results obtained from this study highlighted that the abundance of highly bioactive compounds from Nepeta baytopii ensures the multiple biological activities of the tested extracts, and this suggests a potential use in the pharmaceutical and nutraceutical fields, and therefore should be investigated further.
AtıfScopus32OpenAlex32İncele - MakaleAçık Erişim2021Scopus SJRQ2Exposure to copper and risk assessment for human health via consumption of alcoholic beverages
Ince, Muharrem; Kaplan Ince, Olcay; Onal, Ali
Present study was performed using a Box–Behnken Design (BBD) combined with a response surface methodology (RSM). The ultimate goal of this research was to apply BBD for modeling of copper (Cu2+) ions from alcoholic beverages and evaluate health risk assessment of Cu2+ via consumption of alcoholic beverages. The importance of independent paramaters and their interactions were examined using analysis of variance (ANOVA). Natural clay was investigated to remove Cu2+ ions from some alcoholic beverage samples including beer and wine. X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDS) analysis were used for adsorbent characterization. Same analyzes were applied for Cu2+ loaded clay. In addition, experimental studies showed that Freundlich isotherm was better than Langmuir isotherm. Finally, optimized method was applied to alcoholic beverages. Besides target hazard quotients (THQ), estimated daily intake (EDI) values were calculated, to assess a health risk resulting from these alcoholic beverages regular consumption. The EDI values of Cu (µg/day/person) through consumption of investigated drinks were less than the recommended dietary allowance values. Also, the THQ values of Cu2+ were less than 1. According to estimated target hazard, it can be said no long life health concerns of Cu2+ is associated with the consumption of studied drinks. Obtained results were compared with the data from literature and levels set by regulatory authorities, it can be clearly stated that the consumption of mentioned alcoholic beverages do not pose a toxicological risk.
AtıfScopus14WoS10OpenAlex11İncele - MakaleYalnız künye2021Scopus SJRQ2Two Medicinal Plants (Alkanna trichophila and Convolvulus galaticus) from Turkey: Chemical Characterization and Biological Perspectives
Mahomoodally, Mohamad Fawzi; Zengin, Gokhan; Sinan, Kouadio Ibrahime; Ak, Gunes; Sadeer, Nabeelah Bibi; Angeloni, Simone; Mustafa, Ahmed M.; Caprioli, Giovanni; Maggi, Filippo; Cakilcioglu, Ugur
The aim of the present study was to quantify selected phenolic compounds, determine antioxidant activity and enzyme inhibitory effects of the aerial parts of Alkanna trichophylla Hub.-Mor. (A. trichophylla) and Convolvulus galaticus Rost.ex Choisy (C. galaticus) extracts prepared by homogenizer-assisted extraction (HAE), maceration (MAC) and infusion techniques. This is the first time such study has been designed to validate the phytochemical composition and bioactivity of these plants. Multivariate analysis was conducted on collected data. Rutin and caffeoylquinic acid derivatives were the most significant compounds in A. trichophylla and C. galaticus, respectively. The highest antioxidant activity of A. trichophylla was mostly exhibited by HAE/methanolic extracts as determined by DPPH, ABTS, FRAP (51.39, 112.70 and 145.73 mg TE/g, respectively) and phosphomolybdenum (2.05 mmol TE/g) assays. However, significant antioxidant activities varied within the extracts of C. galaticus. HAE/methanolic extract of A. trichophylla significantly depressed AChE (2.70 mg GALAE/g), BChE (5.53 mg GALAE/g) and tyrosinase (26.34 mg KAE/g) activities and that of C. galaticus inhibited AChE (2.04 mg GALAE/g), tyrosinase (31.25 mg KAE/g) and α-amylase (0.53 mmol ACAE/g) activities significantly. We concluded that HAE was the most efficient extraction technique as high yield of compounds and promising bioactivities were recorded from extracts prepared. Multivariate analysis showed that types of solvents influenced recovery of compounds and biological activities. This research study can be used as one methodological starting point for further investigation on these plants as all results are clearly promising and open the door to further research challenges such as cytotoxicity evaluation, molecular docking analysis, and more screening of pharmacological actions.
AtıfScopus12OpenAlex10İncele - MakaleYalnız künye2021Scopus SJRQ3Glyphosate disrupts sperm quality and induced DNA damage of rainbow trout (Oncorhynchus mykiss) sperm
Akça, Ayşe; Kocabaş, Mehmet; Kutluyer, Filiz
As a widespread pollutant, glyphosate (GLY) adversely affects the aquatic environment and can impair the reproductive ability and functions of fish. The purpose of the current study was to assess in vitro effect of GLY on rainbow trout (Oncorhynchus mykiss) sperm cells. The sperm cells were exposed to different GLY concentrations (2.5, 5, 10 mg/L). Sperm motility parameters were analyzed with computer assisted sperm analysis. DNA fragmentation (%) was measured by the comet assay using fluorescence microscopy. With increased GLY concentration, sperm motility and duration decreased after exposure. DNA fragmentation (% DNA in tail) in sperm cells was higher in treatments containing GLY than control (p < 0.05). Consequently, sperm cells are sensitive to low doses of GLY, and this can negatively affect natural populations.
AtıfScopus8WoS4OpenAlex5İncele - MakaleAçık Erişim2021Scopus SJRQ2Lycopene ameliorates propionic acid-induced autism spectrum disorders by inhibiting inflammation and oxidative stress in rats
Erten, Fusun
This study was conducted to study lycopene efficacy in brain-behavior, pro-inflammatory and apoptotic markers, and antioxidant levels in a rodent model. Rats were administered with propionic acid (PPA) (500 mg/kg BW) to induce autism-like disorders, then treated with different lycopene (L) concentrations (5, 10, 20 mg kg−1 day−1) for 35 days. The groups were: (i);control, (ii);PPA, (iii);PPA + L5, (iv);PPA + L10, and (v);PPA + L20. In this study, serum and brain malondialdehyde (MDA) levels decreased with lycopene supplements compared to the PPA group, similarly to the brain levels of inflammatory factors (IL-1α, IL-8, NF-κB, TNF-α; p <.05). Besides, brain levels of anti-apoptotic Bcl-2 decreased, whereas pro-apoptotic Bax, antioxidant Nrf2, and HO-1 levels in brain increased compared with PPA (p <.05). This study showed that lycopene might have therapeutic value to improve the dysfunctions in learning and memory in a dose-dependent way, along with the antioxidant, anti-inflammatory, and antiapoptotic molecular responses in a rat model of ASD-like disorders. Practical applications: This study suggested that lycopene can reduce propionic acid (PPA)-induced learning and memory impairment and oxidative damage by participating in multiple biological activities such as antioxidant, and anti-inflammatory effects. Lycopene protects serum and brain tissues against PPA induced oxidative damage in rats. These effects may be realized through up-regulation of the brain Nrf2/HO-1 pathway and down-regulation of the IL-1α, IL-8, TNF-α, and NF-κB levels. Lycopene may also contribute to memory and learning function, apoptotic/antiapoptotic modulation, and antioxidant and possible therapeutic efficacy in PPA-induced- Autism spectrum disorder cases.
AtıfScopus26WoS19OpenAlex26İncele - MakaleAçık Erişim2021Scopus SJRQ2In vitro biological activity of Salvia fruticosa Mill. infusion against amyloid β-peptide-induced toxicity and inhibition of GSK-3β, CK-1δ, and BACE-1 enzymes relevant to Alzheimer's disease
Gürbüz, Perihan; Dokumacı, Alim Hüseyin; Gündüz, Miyase Gözde; Perez, Concepcion; Göger, Fatih; Paksoy, Mehmet Yavuz; Yerer, Mükerrem Betül; Ömür Demirezer, L.
Salvia species have been traditionally used to improve cognition and have been proved to be a potential natural treatment for Alzheimer's disease. Salvia fruticosa Mill. (Turkish sage or Greek sage) demonstrated to have anticholinergic effects in vitro. The aim of this study was to understand the mechanism underlying the neuroprotective effects of S. fruticosa infusion and its representative compound rosmarinic acid, which was detected by LC-DAD-ESI-MS/MS. The protective effects of the S. fruticosa infusion (SFINF) and its major substance rosmarinic acid (RA) on amyloid beta 1–42 -induced cytotoxicity on SH-SY5Y cells together with p-GSK-3β activation were investigated. Their in vitro inhibitory effects against glycogen synthase kinase 3β, β-secretase, and casein kinase 1δ enzymes were also evaluated. The results showed that treatment with the all tested concentrations, SFINF significantly decreased Aβ 1–42-induced cytotoxicity and exhibited promising in vitro glycogen synthase kinase 3β inhibitory activity below 10 µg/mL (IC50 6.52 ± 1.14 µg/mL), in addition to β-secretase inhibition (IC50 86 ± 2.9 µg/mL) and casein kinase 1δ inhibition (IC50 121.57 ± 4.00). The SFINF (100 µg/mL and 250 µg/mL) also activated the expression of p-GSK-3β in amyloid beta 1–42 treated SH-SY5Y cells. The outcomes of this study demonstrated that the S. fruticosa infusion possessed activity to prevent amyloid beta 1–42 -induced neurotoxicity and provided proof that its mechanism may involve regulation of p-GSK-3β protein.
AtıfScopus18OpenAlex17İncele - MakaleAçık Erişim2021Scopus SJRQ1Ameliorative effect of Fennel (Foeniculum vulgare) essential oil on chlorpyrifos toxicity in Cyprinus carpio
Pala, Ayşegül; Serdar, Osman; Mişe Yonar, Serpil; Yonar, Muhammet Enis
Chlorpyrifos (CPF) is an organophosphate pesticide that is frequently and widely used to control both agricultural and domestic pests worldwide. In this study, the protective effect of Fennel (Foeniculum vulgare) essential oil (FEO) was investigated in carp (Cyprinus carpio) exposed to CPF. The fish were divided into six groups that one control group (no treatment) and five experimental groups (FEO (3ml/100g diet) group, CPF1 (0.023 mg/l) group CPF2 (0.046 mg/l) group, CPF1 (0.023 mg/l) plus FEO (3ml/100g diet) group, CPF2 (0.046 mg/l) plus FEO (3ml/100g diet) group). Blood and tissue (liver, kidney, gill, and brain) samples were taken from the fish at the end of 14 days of application. Hemoglobin (Hb) level, nitoblue tetrazolium (NBT) activity, and total immunoglobulin (TI) level were measured in blood samples of fish. Acetylcholinesterase (AChE) activity was determined in brain tissue while malondialdehyde (MDA) level, reduced glutathione (GSH) level, catalase (CAT), and glutathione peroxidase (GPx) activity were determined in liver, kidney, and gill tissues. The results showed that there was a significant decrease in Hb level, NBT activity, and TI levels in CPF-treated fish compared to the control group. In addition, increased in MDA levels and significant decreases in GSH level, AChE, CAT, and GPx activities were observed in CPF-treated groups. However, FEO-treated was showed a significant improvement in all parameters except AChE activity compared to CPF groups. These study findings showed that FEO could improve CPF-induced toxicity in C. carpio, except inhibition of AChE activity.
AtıfScopus6WoS6OpenAlex8İncele - MakaleYalnız künye2021Scopus SJRQ2The Effect of the Transtheoretical Model Based Walking Exercise Training and Follow-Up on Improving Exercise Behavior and Metabolic Control in Patients with Type 2 Diabetes
Kaplan Serin, Emine; Citlik Saritas, Seyhan
This study aims to determine the effects of transtheoretical model-based walking exercise training and follow-up on improving exercise behavior and metabolic control in patients with type 2 diabetes. This randomized controlled trial was conducted as a pre-test and post-test experimental model with 76 intervention and 76 control individuals. The intervention group received the transtheoretical model-based exercise training. Data were collected using patient identification form, transtheoretical model scales, pedometer and metabolic outcomes. Data were analyzed by Chi square, two-way ANOVA, Mauchly’s, Greenhouse-Geisser, Friedman, and McNamer test. The overall score means for the exercise change processes, decision-making balance and self-efficacy scales increased compared to the pre-test (p <.001). The mean HDL increased from 41.39 ± 10.35 to 49.18 ± 11.58, and average number of steps per day increased from 3264.31 ± 1933.03 to 5639.37 ± 2317.01. Consequently, this difference between the groups was significant (p <.05).
AtıfScopus11WoS8OpenAlex9İncele - MakaleAçık Erişim2021Scopus SJRQ2Preservice teachers' nomophobia levels, sense of loneliness and adjustment to college life
Çevik-Durmaz, Yadigar; Yalçinkaya-Önder, Eylem; Timur, Serkan
Purpose: The aim of this study was to investigate whether preservice teachers' nomophobia levels, sense of loneliness and adjustment to university life vary based on their demographic characteristics. Additionally, regression analyses were carried out to determine whether their sense of loneliness, nomophobia levels, and adjustment to university life were predictors of each other. Design and Methods: In this quantitative study, the cross-sectional survey research model was used. A total of 234 preservice teachers, 65 of whom were studying at the elementary mathematics department, 81 of whom were studying at the preschool education department, and 88 of whom were studying at the science education department, participated in this study. Independent-samples t test, one-way analysis of variance (ANOVA) and simple linear regression analyses were used to analyze the quantitative data. Findings: The main conclusions from this study were that (a) the students with the highest sense of loneliness were the students of the elementary mathematics department, the students with the highest level of nomophobia were the science education students, and the students who adjusted the most to university life were the preschool education students. (b) There were no significant differences between the 1st and 2nd year preservice teachers in terms of their sense of loneliness, nomophobia levels, and adjustment to university life. (c) There were statistically significant differences between the mean total nomophobia scores of the male and female students in favor of the females especially in the “losing connectedness” and “not being able to access information” subscales of nomophobia. (d) A statistically significant difference was found between the preservice teachers whose mothers were high school and university graduates in terms of their academic adjustment in favor of the preservice teachers with mothers who had university degrees. (e) The preservice teachers, who stated that their relationship with their family was very good, had higher levels of sense of loneliness, mobile phone addiction, and adjustment to university life than the others. The results were statistically significant in all total scores of the loneliness scale and dimensions except for the dimensions of nomophobia. (f) The students who were hopeful for the future had a high sense of loneliness and adjustment to university life. It was also determined that the students who were desperate for their future had high mobile phone addiction levels. Apart from nomophobia and its dimensions and the social adjustment dimension of university life, statistically significant differences were found between the hopeful and hopeless students in terms of their sense of loneliness and adjustment to university life. (g) Statistically significant mean differences were found between the students who were generally satisfied with and those that were not satisfied with their life in terms of their sense of loneliness and adjustment to university life with its all dimensions in favor of the satisfied ones. (h) The preservice teachers who lived alone at home had a higher sense of loneliness and better adjustment to university life. It was also noteworthy that the students living in dormitories had higher nomophobia levels than the others. (i) A linear relationship was found between the students' sense of loneliness and adjustment to university life, and sense of loneliness was a significant predictor of adjustment to university life. Practical Implications: Determining students' nomophobia levels, sense of loneliness, and adjustment to university life would be beneficial in bringing students who have difficulty adapting to new living environments into social life.
AtıfScopus10WoS7OpenAlex16İncele - MakaleAçık Erişim2021Scopus SJRQ2Chemical compositions, radical scavenging capacities and antimicrobial activities in seeds of satureja hortensis l. And mentha spicata l. subsp. spicata from turkey; [Composições químicas, capacidades radicais eliminadoras e atividades antimicrobianas em sementes de satureja hortensis l. E mentha spicata l. subsp. spicata da turquia]
Emre, İ.; Kurşat, M.; Yilmaz, Ö.; Erecevi̇, P.
The present study determined some biological compounds, radical scavenging activity and antimicrobial capacity in seeds of Satureja hortensis L. and Mentha spicata L. subsp. spicata. Alpha-linolenic acid (C18:3 n3) has been found to be the major polyunsaturated fatty acid of Satureja hortensis L. (66.24 ± 1.24%) and Mentha spicata L. subsp. spicata (48.17 ± 1.01%). Linoleic acid (C18:2 n6) is identified as the second major polyunsaturated fatty acid in the present study and oleic acid (C18:1 n9) is determined as the major monounsaturated fatty acid. Current study showed that Satureja hortensis L. and Mentha spicata L. subsp. spicata have low levels of saturated fatty acids. It has been demonstrated that ergosterol (263.1 ± 2.14 µg/g), stigmasterol (39.07 ± 0.91 µg/g) and beta-sitosterol (14.64 ± 0.49 µg/g) have been found in Mentha spicata L. subsp. spicata, while ergosterol (69.41 ± 1.75 µg/g) and beta-sitosterol (19.81 ± 1.14 µg/g) have been determined in Satureja hortensis L. Also, this study determined that Satureja hortensis L. and Mentha spicata L. subsp. spicata have low lipide-soluble vitamin content. Furthermore, it has been found that Satureja hortensis L. contains naringenin (612.57 ± 2.57 µg/g), morin (86.97 ± 1.12 µg/g), quercetin (22.87 ± 0.75 µg/g), and kaempferol (20.11 ± 0.94 µg/g) while naringenin (135.91 ± 1.91 µg/g), naringin (61.23 ± 2.15 µg/g) and quercetin (47.51 ± 1.17 µg/g) have been detected as major flavonoids in the seeds of Mentha spicata L. subsp. spicata. The results of the present study suggest that methanol extracts of Satureja hortensis L. and Mentha spicata L. subsp. spicata have significant free radical scavenging activity. The present results revealed that Satureja hortensis L. and Mentha spicata L. subsp. spicata showed major activity against gram-positive and gram-negative microorganisms, fungi and yeast.
AtıfScopus14OpenAlex18İncele - MakaleYalnız künye2021Scopus SJRQ2Effects of lavender oil intervention before endoscopic retrograde cholangiopancreatography on patients' vital signs, pain and anxiety: A randomized controlled study
Citlik Saritas, Seyhan; Buyukbayram, Zeliha; Kaplan Serin, Emine; Bilgic, Yilmaz
Background: This study was carried out to determine the effects of lavender oil intervention before endoscopic retrograde cholangiopancreatography (ERCP) on patients' vital signs, pain and anxiety. Materials and methods: This study was designed as a randomized controlled study. The population of the study consisted of adult patients who were going to receive ERCP at the gastroenterology clinic of a university hospital. Ninety patients participated in the study. The data were collected using a Patient Information Form, a Pre-Post Test Record Form, VAS and the State Anxiety Scale. Results: After lavender oil intervention, the pulse rate, systolic-diastolic blood pressure, pain and anxiety levels of the patients in the experiment group were reduced, their oxygen saturation levels were increased, and the difference between the groups was statistically significant (p < 0.05). Conclusion: Lavender oil applied on the patients before the ERCP procedure reduced their pulse rate, systolic-diastolic blood pressure, pain and anxiety levels, while it increased their oxygen saturation levels.
AtıfScopus14WoS9İncele - MakaleYalnız künye2021Scopus SJRQ1Development of comprehensive liquid chromatography with diode array and mass spectrometric detection for the characterization of (poly-)phenolic and flavonoid compounds and application to asparagus
Karaaslan Ayhan, Nagihan; Rosenberg, Erwin
The main objective of the present work was to develop a method for the simultaneous and comprehensive analysis of (poly-)phenolic and flavonoid compounds with liquid chromatography with diode array and mass spectrometric detection and its application to green asparagus samples. To this end, a representative set of polyphenols was used to develop the method. A through method validation was carried out with these. The method was applied to asparagus samples known as a healthy vegetable being rich in bioactive compounds. Polyphenol contents of asparagus samples were determined by carrying out quantitative and qualitative analyses by LC-DAD-ESI/MS. In this context asparagus sample extracts were obtained using solvents of different polarity. The results were evaluated statistically and showed that rutin is the major phenolic compound in asparagus. This demonstrates the versatility of this rapid and sensitive method for the simultaneous analysis of (poly-)phenolic and flavonoid compounds which was successfully applied to asparagus samples.
AtıfScopus15WoS15OpenAlex17İncele - MakaleAçık Erişim2021Scopus SJRQ1Magnesium picolinate improves bone formation by regulation of rank/rankl/opg and bmp-2/runx2 signaling pathways in high-fat fed rats
Sahin, Emre; Orhan, Cemal; Balci, Tansel Ansal; Erten, Fusun; Sahin, Kazim
Magnesium (Mg) deficiency may affect bone metabolism by increasing osteoclasts, de-creasing osteoblasts, promoting inflammation/oxidative stress, and result in subsequent bone loss. The objective of the present study was to identify the molecular mechanism underlying the bone protective effect of different forms of Mg (inorganic magnesium oxide (MgO) versus organic magnesium picolinate (MgPic) compound) in rats fed with a high-fat diet (HFD). Forty-two Wistar albino male rats were divided into six group (n = 7): (i) control, (ii) MgO, (iii) MgPic, (iv) HFD, (v) HFD + MgO, and (vi) HFD + MgPic. Bone mineral density (BMD) increased in the Mg supplemented groups, especially MgPic, as compared with the HFD group (p < 0.001). As compared with the HFD + MgO group, the HFD + MgPic group had higher bone P (p < 0.05) and Mg levels (p < 0.001). In addition, as compared to MgO, MgPic improved bone formation by increasing the levels of osteo-genetic proteins (COL1A1 (p < 0.001), BMP2 (p < 0.001), Runx2 (p < 0.001), OPG (p < 0.05), and OCN (p < 0.001), IGF-1 (p < 0.001)), while prevented bone resorption by reducing the levels of RANK and RANKL (p < 0.001). In conclusion, the present data showed that the MgPic could increase osteogenic protein levels in bone more effectively than MgO, prevent bone loss, and contribute to bone formation in HFD rats.
AtıfScopus21WoS20OpenAlex21İncele - MakaleYalnız künye2021Scopus SJRQ1Toxicological effects of di-(2-ethylhexyl) phthalate in Gammarus pulex: a biochemical and histopathological assessment
Yildirim, Nuran Cikcikoglu; Ak, Tuba Parlak; Samasas, Omer
This study aimed to reveal the toxic characteristics of di-(2-ethylhexyl) phthalate (DEHP) by examining the biochemical and histopathological changes in Gammarus pulex, exposed to different doses of DEHP. For this purpose, the lethal concentration 50 (LC50) value of the DEHP was determined by using a static test and found to be 0.079 ± 0.01 ppm. Three subletal doses of DEHP were applied to the G. pulex for 24 and 96 h. Superoxide dismutase (SOD), catalase (CAT), cytochrome P450 1A1 (CYP1A1), and glutathione S-transferase (GST) activities were measured using commercial ELISA kits. The caspase method, which is an immunohistochemical analysis method, was used to determine the apoptosis that occurred in the G. pulex. The results showed that the CYP1A1 activities decreased in the groups exposed to different doses of DEHP compared to the control group (p > 0.05). CAT activity was found to increase in the application groups at the 24 and 96 h compared to the control group. In addition, it was found that SOD and GST activities increased at the 96 h compared to the control group. In light of the microscope examination of the model organism, hemolymphatic lacunae filled with hemolymph and reduction or absence of hemolymphatic ducts were observed especially in the G. pulex gills. Collapse of the gills and hyperplasia were observed after 96 h. As a result, it is suggested that changes in SOD, CAT, and GST activities can potentially be used as sensitive biomarkers for risk assessment in the environment and increased immunoreactivity in G. pulex caused by DEHP depending on increased application doses and application times.
AtıfScopus6WoS3OpenAlex5İncele - MakaleYalnız künye2021Scopus SJRQ1Electrocoagulation induced treatment of indigo carmine textile dye in an aqueous medium: the effect of process variables on efficiency evaluated using biochemical response of Gammarus pulex
Tanyol, Mehtap; Yildirim, Nuran Cikcikoglu; Alparslan, Dilek
Indigo carmine removal from aqueous solutions was investigated by the electrocoagulation process and biochemical responses of Gammarus pulex to solutions before and after treatment were studied. Electrocoagulation experiments in the first stage were carried out with central composite design (CCD) using response surface methodology (RSM). Optimization of process variables that maximize indigo carmine removal by electrocoagulation process was carried out by using a second-order model within the studied test range of various process variables. According to the optimization results, optimum conditions for obtaining 82.55% maximum indigo carmine removal were pH 5.86, a current density of 13.31 mA/cm2, an indigo carmine concentration of 20.01 mg/L, and a time period of 115.80 min. Under optimum conditions for indigo carmine removal, the amount of sludge formed was 0.928 kg/m3, electrode consumption was 0.0305 kg/m3, and energy consumption was 7.461 kWh/m3. The operating cost was calculated as 0.79 US$/m3 under treatment conditions where optimum indigo carmine removal was achieved. In the second stage, G. pulex was exposed to treated and untreated synthetic indigo carmine solutions obtained from experiments according to optimum conditions in order to investigate the biochemical response for 24 and 96 h. Treatment efficiency was evaluated with superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and cytochrome P450 1A1 (CYP1A1) biomarkers. Results clearly showed that the electrocoagulation process was a simple and effective treatment method for the decolorization of wastewaters containing indigo carmine dye. The bioassays used in the present study provided good credibility for checking the detoxification of treated and untreated indigo carmine solutions.
AtıfScopus14WoS12OpenAlex16İncele - MakaleAçık Erişim2021Scopus SJRQ1Comprehensive biological and chemical evaluation of two seseli species (S. gummiferum and s. transcaucasicum)
Zengin, Gokhan; Stojković, Dejan; Mahomoodally, Mohamad Fawzi; Jugreet, Bibi Sharmeen; Paksoy, Mehmet Yavuz; Ivanov, Marija; Gašić, Uroš; Gallo, Monica; Montesano, Domenico
Seseli L. is one of the largest genera of the Apiaceae family widely known for their traditional uses as herbal remedies. In the present study, the methanolic and water extracts of two Seseli species, S. gummiferum and S. transcaucasicum were evaluated for their bioactive contents and biological activities. The total phenolic and flavonoid contents in the extracts ranged from 19.09 to 24.33 mg GAE/g and from 0.45 to10.09 mg RE/g, respectively. Moreover, while narcissin was detected as the most abundant component in the methanolic extract of S. transcaucasicum (261.40 µg/g), chlorogenic acid was identified as the major component in all the other extracts, although a high amount was also present in the methanolic S. transcaucasicum extract (107.48–243.12 µg/g). The total antioxidant capacity was also determined by the phosphomolybdenum assay (0.66–1.18 mM TE/g). Other antioxidant assays such as the radical scavenging assays (DPPH: 5.51–11.45 mg TE/g; ABTS: 43.46–51.91 mg TE/g), reducing assays (CUPRAC: 41.67–53.20 mg TE/g; FRAP: 31.26–34.14 mg TE/g), as well as the metal chelating activity assay (14.38–38.57 mg EDTAE/g) were conducted. All the extracts showed inhibitory potential against the enzyme’s amylase (0.12–0.78 mM ACAE/g), acetyl-and butyryl-cholinesterase (0.15–9.71 mg GALAE/g), while only the methanolic extracts acted as inhibitors of tyrosinase (107.15 and 109.37 mg KAE/g) and only the water extract of S. gummiferum displayed anti-glucosidase activity (0.13 mM ACAE/g). Interestingly, the methanolic extracts of both Seseli species showed lower cytotoxicity towards HaCaT cells (IC50: >500 µg/mL), compared to the water extracts (IC50: 267.8 and 321.41 µg/mL). Besides, only the methanolic extracts showed a slight wound healing effect (28.21 and 31.23%). All extracts showed antibacterial action against Staphylococcus lugdunensis (minimum inhibitory and bactericidal concentrations: 0.025–2 mg/mL). S. gummiferum methanolic extract, which exhibited the highest antibacterial potency was found to inhibit adhesion and invasion of S. lugdunensis to HaCaT cells as well. Taken together, this study demonstrated the two Seseli species to harbour interesting bioactive components, in particular polyphenolics and to exhibit several biological properties that could be further investigated for their potential exploitation as healing agents as supported by various traditional medicinal uses.
AtıfScopus16OpenAlex15İncele - MakaleAçık Erişim2021Scopus SJRQ1Occurrence of micropollutants in the Yesilirmak River Basin, Turkey
Kucuk, Elif; Pilevneli, Tolga; Onder Erguven, Gokhan; Aslan, Sibel; Olgun, Elmas Ö.; Canlı, Oltan; Unlu, Kahraman; Dilek, Filiz B.; Ipek, Ubeyde; Avaz, Gulsen
The European Water Framework Directive (WFD) (2000/60/EC) is the most visionary piece of European environmental legislation that aims to achieve good water status of both surface water and groundwater bodies. The Directive provides a fundamental basis for surface water monitoring activities in the European Member States. The objective of this study is to investigate the occurrence of micropollutants in the Yesilirmak River and to develop a cost-effective monitoring strategy based on spatiotemporal data. A 2-year seasonal monitoring program was conducted between 2016 and 2018, and the water samples were analyzed for 45 priority substances as defined by the WFD and 250 national river basin–specific pollutants. In the basin, 166 pollutants were quantified in at least one of the samples with individual concentrations ranging from 6 × 10−6μg/L to 100 mg/L. Fifty-four pollutants with a frequency of occurrence greater than 5% were selected for further evaluation. Based on statistical evaluation of the data, 20 pollutants were identified as the pollutants of primary concern. These 20 pollutants were grouped under three categories (metals, biocides, and industrial organic compounds) and their spatiotemporal distributions in the basin were assessed to establish a monitoring strategy specific to each pollutant category. The results of the study revealed that the common season for the monitoring of all pollutant categories was the spring. This study provides a generic methodology for the development of a cost-effective water quality monitoring strategy, which can be applicable for use in different basins and pollutant datasets.
AtıfScopus13OpenAlex14İncele - MakaleAçık Erişim2021Scopus SJRQ1Next‐generation ultrasol curcumin boosts muscle endurance and reduces muscle damage in treadmill‐exhausted rats
Sahin, Emre; Orhan, Cemal; Erten, Fusun; Er, Besir; Acharya, Manutosh; Morde, Abhijeet A.; Padigaru, Muralidhara; Sahin, Kazim
Curcumin positively affects performance during exercise and subsequent recovery. However, curcumin has limited bioavailability unless consumed in larger doses. In the current study, we examined the impact of a new formulation of curcumin, Next‐Generation Ultrasol Curcumin (NGUC), which is relatively more bioavailable than natural curcumin on exhaustion time, grip strength, muscle damage parameters, and serum and muscle proteins. A total of 28 rats were randomly grouped as control (C, non‐supplemented), exercise (E, non‐supplemented), E+NGUC100 (supplemented with 100 mg/kg BW NGUC), and E+NGUC200 (supplemented with 200 mg/kg NGUC). Grip strength and exhaustion time were increased with NGUC supplementation (p < 0.0001). Creatine kinase (CK), lactate dehydrogenase (LDH), lactic acid (LA), myoglobin, malondialdehyde (MDA) concentrations were reduced in serum, and muscle tissue in NGUC supplemented groups (p < 0.05). In contrast, NGUC supplementation elevated the antioxidant enzyme levels compared to the non‐supplemented exercise group (p < 0.01). Additionally, inflammatory cytokines were inhibited with NGUC administration (p < 0.05). NGUC decreased PGC‐1α, p‐4E‐BP1, p‐mTOR, MAFbx, and MuRF1 proteins in muscle tissue (p < 0.05). These results indicate that NGUC boosts exercise performance while reducing muscle damage by targeting antioxidant, anti‐inflammatory, and muscle mass regulatory pathways.
AtıfScopus9WoS9OpenAlex9İncele - MakaleAçık Erişim2021Scopus SJRQ1Investigation of perceived fear of COVID-19 and vaccine hesitancy in nursing students
Yeşiltepe, Akgün; Aslan, Sinan; Bulbuloglu, Semra
This study aims to investigate the perceived fear of COVID-19 and vaccine hesitancy in nursing students. This study was conducted as a cross-sectional study with the participation of 1167 nursing students. Personal Information Form, Vaccine Hesitancy Scale and Fear of COVID-19 Scale were used for data collection. The reasons for the vaccine hesitancy of the students were listed as follows: 57.6% of them stated that it had side effects, 17.7% stated there was no conclusive evidence that the vaccine was effective, and 12.6% stated it was not reliable. A positive correlation was found between fear of COVID-19 and vaccine hesitancy.
AtıfScopus19WoS19OpenAlex30İncele