Sürdürülebilir Kalkınma Amaçları

Yazarlar

  • MakaleAçık Erişim2019
    Contributions to The Bryophyte Flora of Hayrat District (Trabzon - Turkey)

    Alataş, Mevlüt; Özen, Öznur; Batan, Nevzat; Erata, Hüseyin

    As a result of bryological excursion carried out in the Hayrat district of Trabzon province, a total of 99bryophyte taxa, including 18 liverworts and 81 mosses, were determined from the samples collected from6 different localities. All of these taxa were reported for the first time from Hayrat district. Brachytheciumcapillaceum (F.Weber & D.Mohr) Giacom. was recorded as a new record for Trabzon province and thesecond record for A4 grid-square.

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  • MakaleAçık Erişim2019
    Epiphytic bryophyte vegetation of Beldibi and Babadağ forests (Zonguldak, Turkey)

    Alataş, Mevlüt; Ezer, Tülay; Batan, Nevzat

    In this study, epiphytic bryophyte vegetation of Beldibi and Babadağ forests has been studied in northwestTurkey (Zonguldak). A total of 55 sample plots were studied. Phytosociological data, obtained from sampe plots,were classified according to the Barun Balnquet method. And also sample plots were ordinated with DetrendedCorrespondance Analysis (DCA) and classified with two-way indicator species analysis (TWINSPAN). Theresults indicated that three bryosociological unit were determined from the forests. These; Neckeretum crispae,Anomodonto viticulosi-Leucodontetum sciuroidis and, Orthotricho straminei-Pterigynandretum filiformis.Amanog them Neckeretum crispae was reported for the first time from Turkey.

    • SDG 15 · Karasal Yaşam
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  • MakaleAçık Erişim2019
    Improvement of Sperm Motility of Rainbow Trout (Oncorhynchus mykiss W., 1792) by Supplementation of L-Arginine

    Danabaş, Durali; Kocabaş, Mehmet; Kutluyer Kocabaş, Filiz

    In this study, the effect of supplementing amino acid-precursor of nitric oxide (NO) L-arginine wereexamined on sperm motility of rainbow trout Oncorhynchus mykiss. Different concentrations [0 mM(Control), 1 mM, 2 mM, 4 mM and 8 mM] of L-arginine were used in the study. To assess the effectson percentage of motile sperm and longevity, L-arginine was added to activation medium containingNaCl (52 mM). The higher L-arginine concentration (4mM) has promoting-effect on sperm motility.The treatments containing L-arginine caused significant effect on percentage of motile sperm andlongevity. Overall, the findings of the present study indicated that supplementation of L-argininemay have improving effects on sperm motility of rainbow trout.

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  • MakaleAçık Erişim2019
    Gölova Baraj Gölü (Sivas)’nde Yaşayan Pullu Sazan (Cyprinus carpio Linnaeus, 1758)’ın Bazı Büyüme Özellikleri ve Et Veriminin İncelenmesi

    Yüngül, Mücahit; Gokhan, Haci Bayram; Dörücü, Mustafa

    Bu çalışmada, Temmuz 2017 - Mart 2018 tarihleri arasında Gölova Baraj Gölü’nden yakalanan 82 adet pullu sazan (Cyprinus carpio Linnaeus, 1758)’ın cinsiyet ve yaş gruplarına göre bazı büyüme özellikleri ile et verimliliği araştırılmıştır. Örneklerin % 32.93’ünü dişi, % 67.07’sini erkek bireyler oluşturmuştur. Dişi ve erkek bireyler II-V arası yaşlarda tespit edilmiştir. Ortalama total boy ve ağırlığın dişilerde 40.99±1.07 cm ve 1001.27±82.97 g; erkeklerde 37.27±0.41 cm ve 734.34±30.69 g; tüm bireylerde 38.49±0.49 cm ve 822.23±36.65 g olduğu saptanmıştır. Tüm örnekler için boy-ağırlık ilişkisi W=0.0151TL2.9743 (R2=0.8796) ve ortalama kondisyon faktörü 1.385±0.018 olarak belirlenmiştir. C. carpio’da et verimliliği ile çeşitli vücut kısımları arasındaki ilişkiler incelendiğinde; fileto ve karkas ağırlıklarının ortalama et verimleri sırasıyla dişilerde % 47.59±0.71 ve % 59.19±0.82; erkeklerde % 49.80±0.32 ve % 60.67±0.34; tüm bireylerde % 49.07±0.34 ve % 60.18±0.36 olarak saptanmıştır. Tüketilmeyen vücut kısımlarından iç organ, baş, yüzgeç, deri ve kemik ağırlıklarının vücut ağırlığına göre ortalama oranları da tüm bireyler için sırasıyla % 12.98±0.39, % 22.32±0.23, % 4.51±0.79, % 8.49±0.25 ve % 2.63±0.04 olarak belirlenmiştir.

    • SDG 2 · Açlığa Son
    • SDG 14 · Sudaki Yaşam
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  • MakaleAçık Erişim2019
    Isparta’da Yetişen Bazı Elma Çeşitlerinin Meyve Eti, Kabuk ve Çekirdek Yuvasındaki Antioksidan Kapasitesinin Belirlenmesi

    Çimen, Murat; Benzer, Fulya; Yıldırım, Mahsum; Barış, Dilan; Yıldırım, Hüseyin; Karaca Sanyürek, Nesrin; Karakavuk, Emrah

    Bu çalışmada Isparta ili Eğirdir ilçesinden temin edilen beş farklı elma çeşidinde meyve eti, kabuk ve çekirdek yuvasındaki antioksidan kapasiteleri ABTS, DPPH ve TFM yöntemleri kullanılarak belirlenmiştir. Analiz sonuçları incelendiğinde çeşitler, alınan kısımlar ve yöntemler bakımından antioksidan seviyelerinin farklılık gösterdiği görülmüştür. En yüksek antioksidan aktivite ‘Granny Smith’ çeşidinde bulunmuştur. En düşük antioksidan aktivite ise ‘Pink Lady’ çeşidinde tespit edilmiştir. Meyve eti, kabuk ve çekirdek yuvasındaki antioksidan miktarları sırasıyla %29.93, %21.95, %27.22 olarak tespit edilirken, TFM metodu ile yapılan analizlerde antioksidan miktarı ABTS ve DPPH metotlarına göre daha yüksek bulunmuştur.

    • SDG 2 · Açlığa Son
    • SDG 3 · Sağlık ve Kaliteli Yaşam
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  • MakaleAçık Erişim2019
    Investigation of the Bioremediation of Herbicide Metribuzin with Bacillus subtilis Bacteria at Artifical Agricultural Field

    Erguven, Gokhan Onder

    The bioremoval of metribuzin (C8H14N4OS) which is one of the selective herbicide in tomato farming in Turkey was investigated with Bacillus subtilis bacteria under agitated culture conditions and artifical agricultural field. The bacterium was isolated in collected soil samples from one of the agricultural area in Elazig province of Turkey. The area is unexposed to metribuzin before. Five apparatuses were set up and 100 mL of metribuzin in 1750 ppm concentration (advised concentration of tomato farmers) was added to each Erlenmayer flasks. 5, 10, 20, 50 and 100 mL of enriched Bacillus subtilis solutions (in sabouraud dextrose broth) added to these flasks. Each mL of the enriched solutions contains ×107 Colony forming unit (CFU). These flasks were shaken at 160 rpm at 28 0C in sterile conditions for 17 days. In 24 hours intervals, each sample was collected to flasks and chemical oxygen demand (COD) was determined with DR/890 colorimeter by the line of closed reflux method while Biochemical oxygen demand (BOD5)experiments were performed via standard methods 5210D with oxitops. As a result of the study, best removal performance observed in 100 mL Bacillus subtilis as 98% at 17 days in COD. In a soil study, 100 mL mixture of Bacillus subtilis consortia used for bioremediation studies. According to the results of artifical agricultural field study, the bioremediation seen at COD and BOD5 parameters are 99.4% and 96% respectively at the end of 2. week. This experiments have focused that, Bacillus subtilis should be a selective type for bioremediation of metribuzin on receiving environments.

    • SDG 2 · Açlığa Son
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  • MakaleAçık Erişim2019
    Effect of Macerated Tomato (Lycopersicon esculentum) and Carrot (Daucus carota) Oils on Hematological Parameters of Rainbow Trout (Oncorhynchus mykiss) at High Stocking Density

    Aksu, Önder; Altınterim, Başar

    In this study, 2% of macerated tomato (Lycopersicon esculentum) and carrot (Daucus carota) oils were added to the feeds of rainbow trout with an average weight of 35.88 ± 1.97 g. The experiment was continued for 21 days and the samples were also measured for changes in the hematological parameters. Statistically, significant differences were found in LYM, RBC, HGB, HCT, MCH and NBT values (p<0.05). There was no statistically significant difference other blood parameters levels (p>0.05). It was determined that both macerated carrot and macerated tomato oils stimulated to the non-specific immune system and erythropoiesis at high stocking density. Especially, it was occurred that macerated carrot oil (MCO) more effective than macerated tomato oil (MTO).

    • SDG 2 · Açlığa Son
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  • MakaleAçık Erişim2019
    Entaurea kurdica Reichardt and Centaurea urvillei DC. subsp. hayekiana Wagenitz’nın Element İçeriği ve Antioksidan Enzimlerinin Belirlenmesi

    Çakılcıoğlu, Uğur; Uruç Parlak, Kadriye

    Kronik hastalıklar dünya çapında hızla artmaktadır. Tüm yaşam boyunca sağlığın iyileştirilmesi ve sürdürülmesinde beslenme ve diyet önemli bir yere sahiptir. Organizmadaki biyokimyasal ve fizyolojik değişim, biyomoleküllerde oksidatif hasara yol açan serbest radikallerin aşırı artmasına neden olabilir. Son zamanlarda tıbbi bitkilerin kullanımı antioksidanlar, antikanser ve hipoglisemik aktiviteler gibi yararlı özellikler nedeniyle artmıştır. Bu çalışmada kullanılan, Centaurea kurdica Reichardt ve Centaurea urvillei DC. subsp. hayekiana Wagenitz (Asteraceae) Elazığ'ın çeşitli bölgelerinde doğal olarak yetişen endemik türlerdir. Centaurea'nın birçok taksonu geleneksel olarak hem ülkemizde hem de dünyada tıbbi amaçlarla ve besinler için kullanılmaktadır. Centaurea kurdica Reichardt ve Centaurea urvillei DC. subsp. hayekiana Wagenitz (Asteraceae) taksonları Elazığ'daki doğal yaşam alanlarından elde edilmiştir. Bitki dokularında ağır metal akümülasyonu, antioksidan enzimler süperoksit dismutaz (SOD), glutatyon S transferaz (GST), askorbat peroksidaz (AP) ve katalaz (CAT) aktiviteleri gibi fizyolojik değişiklikler tespit edilmiştir. Çalışılan enzimlerden SOD en fazla aktiviteye sahip olan enzimdir. Yapılan çalışma sonucunda en fazla akümülasyonun genellikle yapraklarda olduğu tespit edilmiştir. Antioksidan enzim aktiviteleri: süperoksit dismutaz (SOD), glutatyon S transferaz (GST), askorbat peroksidaz (APX) ve katalaz (CAT) değerlendirildi Centaurea kurdica'da sırasıyla 1.315, 0.011, 0.031 ve 0.007 unit mg-1 olarak tespit edildi. C. kurdica'nın Centaurea urvillei DC. subsp. hayekiana’dan askorbat peroksidaz dışında değerlendirilen tüm enzim aktivitelerinde daha iyi olduğu belirlenmiştir.

    • SDG 3 · Sağlık ve Kaliteli Yaşam
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  • MakaleYalnız künye2019
    Changes in Antioxidant and Detoxification Systems of the Freshwater Amphipod Gammarus pulex Exposed to Congo Red

    Serdar, Osman; Tatar, Sule; Cıkcıkoğlu Yıldırım, Nuran

    In this study, it is aimed to investigate the changes in glutathione S-transferase and cytochrome 1A1 enzymes with malondialdehyde levels in Gammarus pulex exposed to congo red. G. pulex was exposed to synthetic solutions containing 20, 10, 5 mg/L of congo red during 96 hours. Glutathione S-transferase and cytochrome 1A1 enzyme activities were determined by using ELISA kit, malondialdehyde levels were determined spectrophotometrically. Glutathione S-transferase activities groups increased in all application groups during 24 and 96 hours compared to control (P<0.05). After exposure to congo red, cytochrome 1A1 activity was decreased during 24 and 96 hours in all application groups compared to control groups (P<0.05). Malondialdehyde levels increased in all groups after congo red exposure compared to control during 24 hours (P<0.05), after 96 hours increased in group A, decreased in groups B and C (P<0.05). Our results show that congo red may cause oxidative stress by producing reactive oxygen species. In conclusion, changes in antioxidant enzymes and malondialdehyde levels suggest that congo red can be used as potential sensitive biomarkers in the assessment of environmental toxicity.

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  • MakaleYalnız künye2019
    Antioxidant Enzyme Responses to Lead Exposure of Gammarus pulex

    Serdar, Osman; Yildirim, Numan; Tatar, Sule; Cıkcıkoğlu Yıldırım, Nuran

    It is aimed to investigate the activity response of the antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase of G. pulex exposed to lead. The G. pulex was exposed synthetic solutions containing 10, 30, 50 µg/L lead for 96 h. Superoxide dismutase, catalase, glutathione peroxidase activities were determined by using ELISA kit. The differences in superoxide dismutase activities were statistically insignificant (P>0.05). Catalase decreased in all treatment groups after lead exposure (P<0.05). The glutathione peroxidase activities were decreased depending on lead exposure (P>0.05). Our findings suggest that lead can induce oxidative stress by generating reactive oxygen species and inhibiting sulfhydryl dependent antioxidant enzymes activities. In conclusion, alterations in antioxidant enzymes may potentially be used as sensitive biomarkers for risk assessment of lead in the environment and may contribute to the establishment of discharge regulations.

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  • MakaleYalnız künye2019
    The Evaluation in Different Temperature of Acute Toxic Effect of Cadmium on Gammarus pulex (Freshwater Amphipoda)

    Serdar, Osman; Çalta, Metin; Aydin, Rahmi

    In this study, the LC50 values of the cadmium (Cd) toxicity on the Gammarus pulex were investigated. In this study, it wasaimed to determine the change of LC50 values of Cd at G. pulex at 10, 14 and 18°C.The lethal concentration (LC50) values for G. pulex whichwere exposed to various Cd concentrations for 96 hours were determined at different temperatures as 10, 14 and 18ºC. LC50 values obtained byprobit analysis were found to be; 51.79 ± 1.2 µg L-1for 10ºC, 47.67 ± 0.6 µg L-1for 14ºC and 33.93 ± 0.6 µg L-1for 18ºC. LC50 values werefound to decrease due to the increase in temperature.

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  • MakaleAçık Erişim2019
    Assessment of vitamin compositions and cholesterol levels of carp (Cyprinus carpio carpio) and rainbow trout (Oncorhynchus mykiss) caviars

    Emir Çoban, Özlem; Ökkeş; Yılmaz, Okkes; Yilmaz; Emine; İnanlı, Ayşe Gürel; Kuzgun, Nermin Karaton

    In this study, roes obtained from carp (Cyprinus carpio carpio) that were caught from Keban Dam Lake (Elazığ) and the culturedrainbow trout (Oncorhynchus mykiss) were processed as caviar and compositions of proximate and vitamins, cholesterol levels were determinedin the products. In the research, roes obtained from mature fish were processed under aseptic conditions by suitable procedures for differentfish species. The obtained caviars were weighed as 75 grams and filled in 200 ml capped glass jar and stored in +4 ºC for 28 days.As a resultof the analysis in the study, the amount of dry matter was determined to be 35.94% in roes obtained from rainbow trout and 31.28% in roesobtained from carp. The average protein content in raw roe obtained from rainbow trout was determined to be 24.32%, it was determined tobe 18.25 % in carp. In this study, the fat content in roes obtained from rainbow trout was determined to be 9.14%, but it did not show muchchange during processing and it was determined to be 8.84% in the end product. During the production and storage of the caviar producedfrom rainbow trout and carp, the fat-soluble vitamin composition and cholesterol levels were determined. During the study, it was found thatthe amounts of vitamin A between rainbow trout and carp caviars were significantly different (p <0.001). Vitamins D and K were not detectedin the caviar samples. As a result of analyzes, cholesterol levels were determined 73.76 mg/100 g in rainbow trout roe and 10.82 mg/100 g incarp roe and it was observed that these values were not changed significantly during the processing and storage periods (on 0th, 7th, 14th, 21stand 28th days ).

    • SDG 2 · Açlığa Son
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  • MakaleAçık Erişim2019
    Congo Red Effects on Detoxification and Metabolic Enzymes in Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792)

    Danabaş, Durali; Yildirim, Nuran Cikcikoglu; Yildirim, Numan; Aksu, Önder

    The aim of this study was to evaluate the activities of the biotransformation enzymes (Glutatyon STransferase (GST) and cytochrome P450 (CYP1A1)) and metabolic enzyme (Lactatedehydrogenase-LDH) in the liver of rainbow trout (Oncorhynchus mykiss) after 24 and 48 hourexposure to 0.5, 1.0 and, 2.0 mg l-1 of Congo red (CR). Enzyme activities were determined by usingcommercial kits with ELISA method. Congo red altered the activities of GST, CYP1A, and LDHenzymes in liver tissue of O. mykiss in a dose-dependent manner. The statistical differences in GSTactivities among the groups for 24 and 48 h were significant, but, LDH activities were significantfor only 24 h. Exposure duration of CR didn’t affect the biochemical response of rainbow trout.Thus, CR exposure changed the biotransformation and metabolic enzymes, and the changes of theseenzymes activities may be used as a potential bioindicator of the CR exposure.

  • MakaleAçık Erişim2019
    Phylogenetic Analysis of Chrysochamela (Fenzl) Boiss. (Brassicaceae) Taxa Growing in Turkey

    Paksoy, Mehmet Yavuz; Sevindik, Emre; Topseçer, Feyzanur; Aydoğan, Melike

    In this study, we performed a genetic diversity analysis using RAPD (Random AmplifiedPolymorphic DNA) and ISSR (Inter simple sequence repeat) markers for some Chrysochamelaspecies growing in Turkey. PCR was performed using RAPD and ISSR primers and genomic DNAsamples from each specimen, and the resulting bands were scored. Phylogenetic relationships andgenetic distances between the studied taxa were calculated using the PAUP analysis program.According to the RAPD analysis, the closest genetic distance was found to be 0.20000 between C.noeana populations, while the most distant values were found as 0.60000 between C. noeana and C.elliptica. The ISSR analysis also revealed the closest genetic distance as 0.34043 between C. noeanapopulations, while the most distant values were found to be 0.55319 between C. noeana and C.elliptica. UPGMA (unweighted pair group method with arithmetic mean) dendrogram generated forboth RAPD and ISSR analyses consisted of two groups. As a result, it was found that RAPD datawere more appropriate for comparison with the previous anatomical and morphological data of the studied taxa.

    • SDG 2 · Açlığa Son
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  • MakaleAçık Erişim2019
    Masere sarımsak (Allium sativum Limne) ve Tunceli sarımsağı (Allium tuncelianum Kollman) yağlarının yoğun stoklanmış gökkuşağı alabalıklarının (Oncorhynchus mykiss W.) bazı kan parametrelerine ve NBT (Nitroblue Tetrazolium) seviyelerine etkileri

    Aksu, Önder; Altınterim, Başar

    Bu çalışmada, % 2 oranında masere sarımsak (Allium sativum L.) ve Tunceli sarımsağı(Allium tuncelianum Koll.) yağları, ortalama ağırlıkları: 39.0 ± 1.64 gr. olan gökkuşağıalabalıklarının yemlerine ilave edilmiştir. Çalışmanın sonunda, gökkuşağıalabalıklarının kan parametreleri ve NBT (Nitroblue Tetrazolium) seviyeleri tespitedilmiştir. Sarımsak uygulaması yapılan gruplarda, MCH, P-LCR, PCT, PLT ve MPVparametrelerinde kontrol ve stres gruplarına göre istatistiksel olarak farklılıkbulunmuştur (P<0.05). WBC, LYM, MID, RBC, HGB, HCT, MCV, MCHC, RDW-SD,RDW-CV ve PDW, değerleri arasında bir farklılık görülmemiştir (P> 0.05). Sarımsakuygulamaları sonucu NBT seviyeleri önemli derecede azalma göstermiştir (P<0.05). Budurum sarımsağın hücrelerdeki oksidatif stresi azalttığını göstermektedir.

    • SDG 2 · Açlığa Son
    • SDG 3 · Sağlık ve Kaliteli Yaşam
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  • MakaleYalnız künye2018Scopus SJRQ3
    Anesthetic Efficiency of 2-Phenoxyethanol on Broodstock of Salmo munzuricus, a New Trout Species Originating from Munzur Stream

    Karataş, Bilal; Basusta, Asiye; Can, Erkan

    Salmo munzuricus Munzur Vadisi Milli Parkı boyunca akan akarsularda yayılım gösteren yeni isimlendirilmiş bir alabalık türüdür. Bu çalışmada, anestetik madde olarak kullanılan 2-fenoksietanolün etkinliği Munzur akarsuyundan yakalanan yerli kahverengi alabalık üzerinde değerlendirilmiştir. Balıklar, 100-200 g (W1), 200-400 g (W2) ve 400-600 g (W3) olmak üzere üç ağırlık sınıfına ayrılmıştır ve çalışma 0.2, 0.3, 0.4 ve 0.5 mL L-1 olmak üzere 4 farklı anestetik konsantrasyonda yürütülmüştür. En kısa indüksiyon zamanı W3 grubunda, en uzun indüksiyon zamanı W1 grubunda bulunmuştur. En kısa anesteziden uyanma süresi W3 grubunda 73.2±3.83 sn sonra ortaya çıkmış ve en uzun uyanma süresi W1 grubunda 136±31.46 sn olarak tespit edilmiştir. W1 grubundaki 2-fenoksietanol konsantrasyonunun artması ile iyileşme süreleri artış göstermiştir. Bununla birlikte, W2 ve W3 gruplarındaki uyanma sürelerinin, W1 grubunda gözlemlenenden genellikle anlamlı derecede daha uzun olduğu belirlenmiştir. Ayrıca balık büyüklüğünün indüksiyon sürelerini ve uyanma sürelerini etkilediği de tespit edilmiştir. Bu çalışmanın sonucu olarak, 2-fenoksietanol için Salmo munzuricus anaçlarında en uygun konsantrasyonlar 100-400 g ağırlık aralığında 0.4 mL L-1, 400-600 g aralığında ise 0.5 mL L-1 olarak belirlenmiştir

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  • MakaleAçık Erişim2018Scopus SJRQ4
    The Effect of Naringenin on Oxidative Stress in Some Tissues (Heart, Lung, Brain, Spleen, Muscle) of Lead-treated Rats

    Benzer, Fulya; Dağ, Üzeyir; Erişir, Mine; Özkaya, Ahmet

    Naringenin, a flavonoid increases resistance against oxidative stress. The present study aims to investigate the effect of lead on lipid peroxidasyonu and some antioxidants in heart, lung, brain, spleen, muscle and the effect of naringenin on these parameters. Naringenin was administered by orogastric gavage (50 mg/kg, dissolved in corn oil) and lead acetate was given as daily 500 parts per million in drinking water for 4 weeks. In the rats exposed to lead, MDA levels in the brain, lungs, spleen and muscle tissues significantly increased (P<0.001), in the heart tended to increase but not significantly (P>0.05). Lead caused a statistically significant decrease in GSH-Px activity of the brain, spleen and muscle tissues, in CAT activity of the heart, lung and muscle tissues, in GSH concentration of the lungs and muscle tissues (P<0.05). The administration of alone naringenin caused a statistically significant decrease in MDA levels in the heart and muscle, but a significant increase in the lung (P<0.05). Although increased MDA levels in the heart, lungs, brain, muscle and spleen tissues were significantly decreased by use of the naringenin together with lead (P<0.05), increased MDA levels in the lungs and spleen tissues could not be recovered to the normal level. Generally the decreased GSH-Px and CAT activities in these tissues due to lead were significanly increased by naringenin supplementation (P<0.05). In addition, the reduced GSH levels in lung and muscle, the inceased GSH levels in heart with effect of lead were returned to the normal by naringenin. Lead particularly causes to an increase in lipid peroxidation by consuming antioxidants of cells. Naringenin was able to prevent as tissue-specific (brain, muscle) the oxidative stress caused by lead.

    • SDG 3 · Sağlık ve Kaliteli Yaşam
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  • MakaleAçık Erişim2018Scopus SJRQ4
    Effects of Different Plant Oils Having Different Oxygen Radical Absorbance Capacity (ORAC) on Hematological Parameters of Rainbow Trout (Oncorhynchus mykiss) at High Stocking Density

    Aksu, Önder; Altınterim, Başar; Kutluyer Kocabaş, Filiz

    In this study, macerated oils of different plant [sunflower (Helianthus annuus), onion (Allium cepa), garlic (Allium sativum), basil (Ocimum basilicum), opium poppy (Papaver somniferum), ginger (Zingiber officinale), turmeric (Curcuma longa)] were obtained. The study was conducted at a total of 7 groups, including the control group (sunflower oil), in 30 liter aerated tanks without water circulation during a week. Twenty rainbow trout per tank (14.6±1.0 g) were stocked at high density. Macerated oils were applied to the skin of the fish along the lateral line on the first day. For determination of effects of macerated oils on water and fish, oxidationreduction potential (ORP) of water and hematological parameters [granulocyte (GRAN), white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), mean corpuscular hemoglobin (MCH), red cell distribution width (RDW-SD), mean platelet volume (MPV), platelet distribution width (PDW), platelet large cell ratio (P-LCR)] were examined in fish. The results indicated that decreases were determined in ORP level of water. It was determined that the highest survival rate was in the application of basil oil. At the end of 7 days, the highest WBC, LYM, HGB, MCV, MCH, MPV values were determined in the application of ginger oil (ORAC: 28.811). It was determined that the highest RBC value was in the application of turmeric oil (ORAC: 159.277), the highest GRAN value was in the application of poppy oil (ORAK: 481), the highest HCT, PLT values was in the application of basil oil (ORAC: 67.553). At the end of the study, it was determined that the macerated oil application to lateral line induced blood parameters.

    • SDG 2 · Açlığa Son
    • SDG 3 · Sağlık ve Kaliteli Yaşam
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  • MakaleYalnız künye2018Scopus SJRQ3
    The protective effects of propolis and flunixin meglumine on feed intake, antioxidant status and histological parameters in liver and kidney tissues against excess copper in rats

    Parlak Ak, Tuba; Baykalır, Burcu Gül; Türkmen, Neşe Başak; Seven, Pinar Tatli; Seven, Ismail; Yaman, Mine

    This experiment was conducted to determine the protective effects of flunixin meglumine and propolis on feed intake, antioxidant status and histological parameters in kidney and liver tissues in rats exposed to excessive copper. In this study individually housed thirty-six male Sprague-Dawley rats were used. Animals were randomly divided into six groups; control, copper sulphate (500 mg/kg BW/day, gavage), flunixin meglumine (2.2 mg/kg BW/day, ip), propolis (100 mg/kg BW/day, gavage), copper sulphate+flunixin meglumine (500 mg/kg BW/day of copper sulphate by gavage and 2.2 mg/kg BW/day of flunixin meglumine, ip) and copper sulphate+propolis (500 mg/kg BW/day of copper sulphate and 100 mg/kg BW/day of propolis by gavage). The study demonstrated that body weight change in the copper sulphate+propolis group significantly ameliorated in comparison with copper group (P<0.01). Propolis and flunixin meglumine administration significantly decreased MDA levels in the kidney and liver tissues, and serum TNF-α levels (P<0.001). Propolis supplementation to rats who were also treated with copper significantly increased the superoxide dismutase, catalase and reduced glutathione activities (P<0.001). Flunixin meglumine and propolis treatments decreased the copper-induced degenerative and necrotic changes with the apoptotic cells in the liver and kidney tissues. In conclusion, propolis appeared to ameliorate the adverse effects on feed intake, liver and kidney tissues seeing in the copper treatment and, apparently caused by the copper toxicity, by scavenging the free radicals and increasing activity of antioxidants.

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  • MakaleAçık Erişim2018
    Gökkuşağı alabalığı (Oncorhynchus mykiss) ve Çoruh alabalığı (Salmo coruhensis) sperm motilitesinin başlamasında pH’ın rolü

    Kutluyer Kocabaş, Filiz

    Çoruh alabalığı (Salmo coruhensis) ve gökkuşağı alabalığının (Oncorhynchus mykiss) sperm motilitesi üzerinde farklı aktivasyon solusyonlarının pH (6.0–9.0)’ının etkilerini belirlemek için denemeler dizayn edilmiştir. Dört aktivasyon solusyonu hazırlanmıştır: a) NaCl (52 mM)-AS1, b) NaCl (103 mM), CaCl2 (63 mM), Glikoz (4 mM), Tris (10 mM)-AS2, c) NaHCO3 (119 mM)-AS3, d) NaCl (45 mM), KCl (5 mM), Tris (30 mM)-AS4. Sperm farklı aktivasyon solusyonlarıyla 1:100 sulandırma oranıyla aktive edilmiştir. S. coruhensis türünde en yüksek motilite oranı ve süresi pH 8’den elde edilirken O. mykiss türünde en yüksek motilite oranı ve süresi AS2 solusyonu pH 9’da elde edilmiştir. Veriler, iki tür için AS2’de ph 6-8 aralığında non-progresif motilite olduğunu göstermiştir. Sonuç olarak, sperm motilitesi ve süresi düşük pH değerlerinden olumsuz etkilemesine rağmen, Çoruh alabalığı S. coruhensis ve gökkuşağı alabalığının O. mykiss spermi geniş pH aralıklarında motil olabilir.

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